I have an expression matrix containing RSEM values for genes. I want to run DESeq2 on these file using DESeq2 package. Since the RSEM values are values like "54.87632", I multiplied all the values with 10000 to make all of them integers. Then, I uploaded both the expression data and the metadata. I checked the colnames/rownames. Then, I run the code and having the error:
dds <- DESeqDataSetFromMatrix(countData = expression, colData = metadata, design = ~ Group) #then the error: converting counts to integer mode NAs introduced by coercion to integer rangesome variables in design formula are characters, converting to factorsError in validObject(.Object) : invalid class “DESeqDataSet” object: NA values are not allowed in the count matrix
Since I dont have any missing value:
anyMissing(expression)  FALSE
I don`t know how to correct my data/what is wrong about my data. Is there anyone who have the same issue/solution for this problem?
Thank you for your reccomendations!