Differential expression on RNAseq without replicates
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Themis • 0
@Themis-24410
Last seen 25 days ago

I have managed to sequence the transcriptome of single replicates of very rare samples with low yield so technical or biological replicates are almost impossible to obtain. I have used salmon to quantify the expression and inferred 10 bootstrap replicates of each Which approach is the best one to use for downstream analysis? I know that significance without variance is really unreliable, but I have to get an estimation of the results. Thank you

DESeq2 NOISeq edgeR • 97 views
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Hey, I just want to add here that there are many related questions over at biostars, see for pointers: https://www.biostars.org/p/480680/ Please go through existing threads, this has been asked many times before, and please feel free to comment on existing threads if something is unclear, but be sure to read the available material before, since as said, this has been discussed many times before.

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@mikelove
Last seen 2 days ago
United States

DESeq2 is not possible without replicates.

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