Question

DiffBind 'mc.cores' > 1 is not supported on Windows

0

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GM ▴ 10

@gm

Last seen 2.6 years ago

Sint-Martens-Latem

Hello,

I have a problem with Diffbind. After the command:

DB_greylist <- dba.blacklist(DB, greylist=T )

I get the following error:

Error in mclapply(seq_len(reps), fitDist, obj@counts, size, mc.cores = cores) : 
  'mc.cores' > 1 is not supported on Windows

I tried mc.cores=1 but didn't work.

Thank you for your time.

R version 4.0.3 (2020-10-10)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 18363)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252    LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                           LC_TIME=English_United States.1252    

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] DESeq2_1.30.0               GenomeInfoDbData_1.2.4      dplyr_1.0.2                 DiffBind_3.0.4             
 [5] SummarizedExperiment_1.20.0 Biobase_2.50.0              MatrixGenerics_1.2.0        matrixStats_0.57.0         
 [9] GenomicRanges_1.42.0        GenomeInfoDb_1.26.0         IRanges_2.24.0              S4Vectors_0.28.0           
[13] BiocGenerics_0.36.0        

loaded via a namespace (and not attached):
  [1] backports_1.2.1          GOstats_2.56.0           BiocFileCache_1.14.0     plyr_1.8.6               GSEABase_1.52.0         
  [6] splines_4.0.3            BiocParallel_1.24.1      ggplot2_3.3.3            amap_0.8-18              digest_0.6.27           
 [11] invgamma_1.1             htmltools_0.5.0          GO.db_3.12.1             SQUAREM_2020.5           magrittr_2.0.1          
 [16] checkmate_2.0.0          memoise_1.1.0            BSgenome_1.58.0          base64url_1.4            limma_3.46.0            
 [21] Biostrings_2.58.0        readr_1.4.0              annotate_1.68.0          systemPipeR_1.24.2       askpass_1.1             
 [26] bdsmatrix_1.3-4          prettyunits_1.1.1        jpeg_0.1-8.1             colorspace_2.0-0         blob_1.2.1              
 [31] rappdirs_0.3.1           apeglm_1.12.0            ggrepel_0.9.0            xfun_0.20                crayon_1.3.4            
 [36] RCurl_1.98-1.2           jsonlite_1.7.2           graph_1.68.0             genefilter_1.72.0        brew_1.0-6              
 [41] survival_3.2-7           VariantAnnotation_1.36.0 glue_1.4.2               gtable_0.3.0             zlibbioc_1.36.0         
 [46] XVector_0.30.0           DelayedArray_0.16.0      V8_3.4.0                 Rgraphviz_2.34.0         scales_1.1.1            
 [51] pheatmap_1.0.12          mvtnorm_1.1-1            DBI_1.1.0                edgeR_3.32.0             Rcpp_1.0.5              
 [56] xtable_1.8-4             progress_1.2.2           emdbook_1.3.12           bit_4.0.4                rsvg_2.1                
 [61] AnnotationForge_1.32.0   truncnorm_1.0-8          httr_1.4.2               gplots_3.1.1             RColorBrewer_1.1-2      
 [66] ellipsis_0.3.1           pkgconfig_2.0.3          XML_3.99-0.5             dbplyr_2.0.0             locfit_1.5-9.4          
 [71] tidyselect_1.1.0         rlang_0.4.10             AnnotationDbi_1.52.0     munsell_0.5.0            tools_4.0.3             
 [76] generics_0.1.0           RSQLite_2.2.1            evaluate_0.14            stringr_1.4.0            yaml_2.2.1              
 [81] knitr_1.30               bit64_4.0.5              caTools_1.18.0           purrr_0.3.4              RBGL_1.66.0             
 [86] xml2_1.3.2               biomaRt_2.46.0           compiler_4.0.3           rstudioapi_0.13          curl_4.3                
 [91] png_0.1-7                geneplotter_1.68.0       tibble_3.0.4             stringi_1.5.3            GenomicFeatures_1.42.0  
 [96] lattice_0.20-41          Matrix_1.2-18            vctrs_0.3.6              pillar_1.4.7             lifecycle_0.2.0         
[101] irlba_2.3.3              data.table_1.13.6        bitops_1.0-6             rtracklayer_1.50.0       R6_2.5.0                
[106] latticeExtra_0.6-29      hwriter_1.3.2            ShortRead_1.48.0         KernSmooth_2.23-17       MASS_7.3-53             
[111] gtools_3.8.2             assertthat_0.2.1         openssl_1.4.3            Category_2.56.0          rjson_0.2.20            
[116] withr_2.3.0              GenomicAlignments_1.26.0 batchtools_0.9.14        Rsamtools_2.6.0          hms_0.5.3               
[121] grid_4.0.3               DOT_0.1                  coda_0.19-4              rmarkdown_2.6            GreyListChIP_1.22.0     
[126] ashr_2.2-47              mixsqp_0.3-43            bbmle_1.0.23.1           numDeriv_2016.8-1.1      tinytex_0.28

DiffBind • 2.3k views

ADD COMMENT • link 3.3 years ago GM ▴ 10

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I will permit that the developer of DiffBind responds, as there may be a fix about which I am unaware. Please show the command that you ran prior to the error appearing. Also add the output of sessionInfo() to your original question.

Windows cannot implement compute parallelisation in the same way as on MAC / Linux. So, on Windows, you cannot use mc.cores > 1. On Windows, pseudo-compute parallelisation can be achieved via SNOW by registering a cluster object and using parLapply(), as to which I elaborate here on Biostars: Tutorial: Parallel processing in R

ADD REPLY • link 3.3 years ago Kevin Blighe ★ 3.9k

1

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Thanks, I add the details in my post.

ADD REPLY • link 3.3 years ago GM ▴ 10

score 0 · Answer 1 · 2021-01-08

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Rory Stark ★ 5.1k

@rory-stark-5741

Last seen 7 days ago

Cambridge, UK

DiffBind does not support parallel operation on Windows. There is an issue when building greylists (greylist=TRUE by default when calling dba.blacklist) within the GreyListChIP package on Windows.

You can set cores=1 when calling dba.blacklist(), or set

DB$config$cores <- 1

to avoid this problem. I will put out an update soon to catch the case where it is trying to use more cores on Windows so as to avoid the error.

Note that it can take a long time to generate greylists in this way!

Also, you should update to the latest version of DiffBind (currently 3.0.9) as there have been important bug fixes.

ADD COMMENT • link 3.3 years ago Rory Stark ★ 5.1k

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Thanks for the reply. I was about to comment on my post that, when I set the cores to 1 in the DB I get the error:

Error in sample.int(length(x), size, replace, prob) : 
  cannot take a sample larger than the population when 'replace = FALSE'

ADD REPLY • link 3.3 years ago GM ▴ 10

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I'm pretty sure that this is a separate issue, unrelated to the parallel one. Do you have more of the output? It should have printed out which control file was being processed when the error occurred.

If you could give me access to the control bam file that is being used to generate the greylist, I can track it down with Gord Brown, who wrote the GreyListChIP package.

In the mean time it would be good if you could update to 3.0.9 so we know this isn't impacted by more recent fixes.

ADD REPLY • link 3.3 years ago Rory Stark ★ 5.1k

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I update to 3.0.9. The error is on the first control sample. How can I send you the file?

ADD REPLY • link 3.3 years ago GM ▴ 10

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I assume it is pretty big (more than a few MB), the best way to to put it on a Dropbox or other server and send me a link.