To the proActiv Team,
I am currently using proActiv on my RNA-seq data set. I was wondering if you could explain:
1) Why you chose to do the junction read count method compared to other methods for proActiv?
2) Why did you not normalise for intron length when calculating Absolute Promoter activty?
3) Why you chose an absolute activity of 0.25 as the cut off value of inactive promoters vs minor promoters?
All the Best,