Hello, my name is Jonathan I'm new to R and to RNA-seq. I have a question regarding a project I'm working on. I want to compare differential expression of RNA within 4 different groups of cells (different tumors). can I use DESEQ2 for that even though the cell are not paired or is there any other tool in order to do so? if someone can link me a manual for that kind of analysis I would be grateful. thank you for your support, Jonathan.