GTF annotation file for featurecount
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Ashish • 0
@Ashish-24782
Last seen 9 weeks ago

Hi, I am new to this, for the diffrential gene expression analysis i have aligned the trimmed raw reads to the master RNAseq data by using Bowtie2. this generated a SAM file which i converted to BAM file format. Now i want to count the reads by using Rsubread function featurecounts, This requires gtf annotation file. how can i generate the gtf file for this.

Thank you

SeqGate TarSeqQC ExperimentHubSoftware projectR • 70 views
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What is "master RNAseq data"? What is your organism?

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