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10
views
Question on RNA-Seq single sample data analysis in EdgeR
R
edgeR
limma
RNASeq
Normalization
48 minutes ago
mohammedtoufiq91
▴ 10
0
votes
1
reply
16
views
regression analysis for epic array methylation data using limma
limma
Epigenetics
STATISTICS
R
updated 1 hour ago by
Basti
▴ 280 • written 1 hour ago by
nabiyogesh
▴ 10
2
votes
6
replies
117
views
Warnings when using "ashr" in lfcshrinkage for DESeq2
RNASeq
DESeq2
1 day ago • updated 2 hours ago
andrebolerbarros
▴ 10
0
votes
1
reply
23
views
scRNASeq reference at a transcript level
EWCE
scRNAseq
updated 2 hours ago by
Basti
▴ 280 • written 3 hours ago by
marc.tormo
• 0
0
votes
1
reply
29
views
How to run GSVA with partially NA genes
GSVA
updated 6 hours ago by
Robert Castelo
★ 2.8k • written 9 hours ago by
blakebowen
• 0
0
votes
0
replies
21
views
error with: .make_splicings <- function(exons, cds, stop_codons=NULL)
GenomicFeatures
14 hours ago
rsnorman
• 0
0
votes
2
replies
176
views
Looking for a package that compares my RNA seq data to find published dataset with a similar expression pattern
RNASeq
written 18 days ago by
curiousmind007
• 0
0
votes
2
replies
74
views
Using limma to read Illumina HT-12 v4 data
limma
updated 1 day ago by
Gordon Smyth
45k • written 1 day ago by
orbitofrontal
• 0
0
votes
4
replies
108
views
performing the differential binding in ChIP-seq
ChIP-seq
updated 1 day ago by
ATpoint
★ 1.2k • written 3 days ago by
Bogdan
▴ 640
0
votes
0
replies
28
views
CPM after RUVseq
RUVSeq
CPM
Normalization
1 day ago
Jess
• 0
0
votes
2
replies
53
views
R: problem with running time-series analysis software: Mfuzz
time-series
mfuzz
replace=FALSE
2 days ago
soda
▴ 80
0
votes
1
reply
61
views
ashr vs apeglm for interaction data
lfcShrink
DESeq2
updated 2 days ago by
Michael Love
37k • written 4 days ago by
Ali Barry
▴ 30
2
votes
4
replies
86
views
Robust Multi-array Average could get negative value or not?
pd.hg.u219
oligo
AffymetrixChip
2 days ago
Yang Shi
• 0
1
vote
5
replies
115
views
UniprotKB AC/ID to Entrez ID Conversion
ID
Uniprot
EntrezID
3 days ago • updated 5 hours ago
tom.kloter
• 0
2
votes
2
replies
67
views
how to make a group to apply the data to CAMERA
CAMERA
3 days ago • updated 1 day ago
Akira
• 0
4
votes
10
replies
190
views
Methods for removing unexpressed probes of Affymetrix Human Genome U219 Array
pd.hg.u219
Microarray
oligo
AffymetrixChip
4 days ago • updated 3 days ago
Yang Shi
• 0
4
votes
6
replies
155
views
How to best visualize multi-level transcriptomics experiment data?
ggplot2
Transcriptomics
Visualization
R
limma
5 days ago • updated 6 hours ago
mohammedtoufiq91
▴ 10
1
vote
4
replies
189
views
different results in DESeq2 when comparing groups in two ways
DESeq2
4 days ago • updated 3 days ago
zohreh.fazelan
• 0
0
votes
1
reply
87
views
Function Request for Rsubread or tximport: parsing command-line featureCounts output
tximport
Rsubread
updated 3 days ago by
ATpoint
★ 1.2k • written 3 days ago by
johnmcma
▴ 10
1
vote
1
reply
63
views
Is it necessary to standardize Combat in R?
RNA
Combat
sva
R
4 days ago • updated 3 days ago
takemoto
• 0
3
votes
5
replies
113
views
Print EnhancedVolcano Plot as jpeg or similar in R Notebook
EnhancedVolcano
updated 3 days ago by
Kevin Blighe
3.5k • written 4 days ago by
Bine
▴ 20
0
votes
0
replies
31
views
Regarding installing RAMA, BRIDGE, and iterative BMA packages in MultiExperiment Viewer (MeV).
MeV+R
MeV
4 days ago
Souvik
• 0
5
votes
5
replies
2.3k
views
how to understand cpm=1 and how filterByExpr works?
edger
updated 4 days ago by
gowthamee
• 0 • written 20 months ago by
linouhao
▴ 20
0
votes
1
reply
54
views
after calcNormFactors with EdgeR, how to get TMM normalized matrix?
Normalization
edgeR
updated 4 days ago by
Gordon Smyth
45k • written 4 days ago by
kevin
• 0
3
votes
4
replies
117
views
Quantile Normalization after rma or not?
limma
Microarray
oligo
4 days ago • updated 3 days ago
Yang Shi
• 0
1
vote
1
reply
64
views
Using featureCounts and downloading Rsubread
featureCounts
RNASeq
Rsubread
R
updated 2 days ago by
Gordon Smyth
45k • written 4 days ago by
margo
• 0
2
votes
1
reply
58
views
Get MANE transcripts from TxDb.Hsapiens.UCSC.hg38.knownGene
AnnotationDbi
updated 4 days ago by
James W. MacDonald
59k • written 5 days ago by
nhaus
• 0
0
votes
3
replies
159
views
methylated/unmethylated media threshold (EPIC assay)
IlluminaHumanMethylationEPICanno.ilm10b3.hg19
ChAMP
minfi
7 weeks ago • updated 5 days ago
ramonfallon
• 0
0
votes
1
reply
74
views
ChIP-seq peak annotation to gene in ChIPseeker
ChIPseeker
5 days ago • updated 2 days ago
Gyan Prakash Mishra
• 0
0
votes
1
reply
53
views
champ.SVD() error, assumes standard class() function will return single string
ChAMP
updated 5 days ago by
Basti
▴ 280 • written 5 days ago by
ramonfallon
• 0
3
votes
3
replies
108
views
edgeR: the F-statistics changed after removing lowly expressed gene
glmQLFTest
edgeR
updated 5 days ago by
Gordon Smyth
45k • written 5 days ago by
Pei
• 0
0
votes
13
replies
298
views
EnrichGO not working with db created from annotationhub
clusterProfiler
AnnotationHubData
GO
updated 5 days ago by
Guido Hooiveld
★ 3.2k • written 8 days ago by
Lucía
• 0
2
votes
1
reply
63
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 5 days ago by
swbarnes2
★ 1.0k • written 5 days ago by
Hicham
• 0
0
votes
0
replies
28
views
Rcpp std::chdir
Rcpp
5 days ago
ullrich
▴ 10
0
votes
1
reply
59
views
DESeq2 paired design: Differential list comparing fold changes
DESeq2
updated 5 days ago by
swbarnes2
★ 1.0k • written 5 days ago by
athira
• 0
0
votes
3
replies
103
views
DiffBind: 0 consensus peaks for dba.peakset: New bug / undocumented change introduced between version 2.14 and 3.4.11
DiffBind
updated 5 days ago by
Rory Stark
★ 4.4k • written 6 days ago by
chrarnold
• 0
0
votes
0
replies
36
views
RPX PXDataset error
PRIDE
rpx
PXDataset
5 days ago
gianluigi.caltabiano
• 0
3
votes
4
replies
151
views
DESeq2: Extracting Averaged Counts Information from dds and rld
DESeq2
11 days ago • updated 5 days ago
vanbelj
▴ 10
0
votes
6
replies
195
views
DESeq2, Calculate the size factor by using median-of-ratio
median
DESeq2
10 days ago • updated 5 days ago
KEREN
• 0
2
votes
2
replies
79
views
Methods for removing unexpressed probes of Human Transcriptome Array HTA 2.0
hta20transcriptcluster.db
Microarray
oligo
6 days ago
Yang Shi
• 0
2
votes
2
replies
80
views
rtracklayer S.h
install
7 days ago • updated 5 days ago
gibran
• 0
5
votes
4
replies
326
views
HTSeq Counts no longer available
TCGAbiolinks
updated 6 days ago by
swbarnes2
★ 1.0k • written 7 weeks ago by
vm
▴ 30
0
votes
1
reply
88
views
The results(dds) function in DESeq2 gives Na values for padj
DESeq2
updated 7 days ago by
ATpoint
★ 1.2k • written 7 days ago by
keshav.prasad.gubbi
• 0
0
votes
0
replies
49
views
LEA get Q-matrix from best snmf() run
LEA
7 days ago
janis
• 0
0
votes
4
replies
128
views
featureCounts: 0% successfully assigned fragments
featureCounts
7 days ago • updated 4 days ago
KMS
• 0
0
votes
1
reply
55
views
AssignChromosomeRegion not working in Rstudio 4.2
ChIPpeakAnno
ChIPSeq
updated 6 days ago by
Ou, Jianhong
★ 1.3k • written 7 days ago by
Abhilansh
• 0
0
votes
3
replies
330
views
ChIPQC: Depths of coverage plot - Figure 1 of the ChIPQC report
ChIPQC
written 10 months ago by
etiennedanis
• 0
2
votes
2
replies
98
views
Handling NA values in limma's voom design matrix
limma
voom
design
NAs
updated 8 days ago by
Gordon Smyth
45k • written 8 days ago by
dr
▴ 10
0
votes
1
reply
78
views
miRNA analysis with DESeq2
mirna
DESeq2
updated 9 days ago by
Michael Love
37k • written 9 days ago by
Faith.omonijo
• 0
0
votes
4
replies
155
views
different sample size from each individual
DESeq2
updated 9 days ago by
Michael Love
37k • written 9 days ago by
LW
• 0
1,000 results • Page
1 of 20
Recent ...
Replies
Comment: regression analysis for epic array methylation data using limma
by
Basti
▴ 280
Which package do you intend to use ? There are many tutorials in packages vignette that show you how to perform differential methylation an…
Comment: scRNASeq reference at a transcript level
by
Basti
▴ 280
I believe this question is not related to any Bioconductor package, I think the only solution to this question is to search yourself in Pub…
Comment: Warnings when using "ashr" in lfcshrinkage for DESeq2
by
andrebolerbarros
▴ 10
Upgrading the package solved the issue, thanks!
Answer: Warnings when using "ashr" in lfcshrinkage for DESeq2
by
ATpoint
★ 1.2k
The error comes from the `mixsqp` package, see https://github.com/stephens999/ashr/issues/131. Upgrading the package to the most recent 0.3…
Comment: Warnings when using "ashr" in lfcshrinkage for DESeq2
by
andrebolerbarros
▴ 10
Thanks for the suggestion. I ran the following filtering criteria: ``` keep <- rowSums(counts(dds) >= 10) >= 5 dds <- dds[keep,] ``` It ga…
Votes
Answer: Warnings when using "ashr" in lfcshrinkage for DESeq2
Answer: Warnings when using "ashr" in lfcshrinkage for DESeq2
Answer: UniprotKB AC/ID to Entrez ID Conversion
edgeR: what to do with no replicates
Differential gene expression anlaysis?
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