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Filtering (removing lowly expressed genes) is separate to normalization.
It isn't clear why not subsetting would cause any difficulty. It …
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Thank you for your suggestion. Yes, I have made DGEList and design matrix in my script. But I was not entirely confident while making thos…
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Hello Gordon,
Thanks for your response. By normalization, I meant that I have already removed lowly expressed genes from my count matrix. …
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It doesn't seem to me that there is necessarily any problem with the analysis.
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Your question is curious because you showed code that made a DGEList and design matrix in your previous post just 15 hours ago.
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