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Votes
34
votes
21
replies
14k
views
DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
deseq2
9.6 years ago • updated 19 months ago
Michael Love
41k
32
votes
0
replies
38k
views
limma moderated t-statistics and B-statistics
Microarray
limma
19.6 years ago • updated 3.5 years ago
Gordon Smyth
50k
18
votes
4
replies
1.6k
views
`ggbio` package can't be loaded
ggbio
3.3 years ago
Batool
▴ 370
16
votes
0
replies
3.7k
views
simultaneous use of robust and weighting methods in limma
limma
robust
arrayWeights
10.4 years ago • updated 7.9 years ago
Gordon Smyth
50k
14
votes
10
replies
3.7k
views
Error when trying to preprocessReads() : "Error: logical subscript contains NAs"
systempiper
5.4 years ago
Deleted
▴ 70
14
votes
1
reply
1.9k
views
it paused in line **byte-compile and prepare package for lazy loading** when install package EnsDb.Hsapiens.v75
EnsDb.Hsapiens.v75
updated 3.3 years ago by
Batool
▴ 370 • written 3.3 years ago by
YunYun
▴ 70
12
votes
2
replies
1.4k
views
`TxDb.Hsapiens.UCSC.hg19.knownGene` and `EnsDb.Hsapiens.v75` can't be installed anymore
EnsDb.Hsapiens.v75
TxDb.Hsapiens.UCSC.hg19.knownGene
3.3 years ago
Batool
▴ 370
10
votes
8
replies
2.9k
views
Best alternative annotation package to BiomaRt
R
biomaRt
Ensembl
rnaseq
4.8 years ago
m93
▴ 10
8
votes
4
replies
6.9k
views
Can't upgrade to Bioconductor 3.3?
bioconductor
biocupgrade
upgrade
update
bioclite
8.0 years ago
enricoferrero
▴ 660
8
votes
2
replies
3.0k
views
exprs function - clash with dplyr
exprs
dplyr
Biobase
5.9 years ago
Wolfgang Huber
★ 13k
8
votes
7
replies
1.1k
views
Why is DMRcate not available for Bioconductor version 3.18?
DMRcate
written 4 months ago by
Daniel E. Weeks
▴ 30
7
votes
2
replies
1.9k
views
False discovery rates in DEP
p-value
FDR
DEP
updated 2.8 years ago by
António Miguel de Jesus Domingues
▴ 490 • written 3.4 years ago by
Alexei Leliavski
▴ 60
7
votes
9
replies
1.7k
views
codelink package is not installing
codelink
r
9.0 years ago
Agaz Hussain Wani
▴ 260
7
votes
6
replies
2.0k
views
Help to find Volunteer who can review my bioconductor package before submission ?
r packages
submission
8.2 years ago
Jurat Shahidin
▴ 80
7
votes
8
replies
3.7k
views
R failing to load genome files from EnsDb.Hsapiens.v86
tximport
ensembldb
EnsDB.hsapiens.v86
updated 5.3 years ago by
Bioconductor Community
0 • written 5.4 years ago by
michaelschoeber
▴ 20
6
votes
3
replies
1.3k
views
MEDIPS for chIP-Seq
ChIPSeq
written 20 months ago by
Darek
▴ 30
6
votes
6
replies
6.8k
views
Avoid upgrading BiocManager to 1.30.13; fix proposed
BiocManager
updated 2.7 years ago by
Mengfei
• 0 • written 3.0 years ago by
Vincent J. Carey, Jr.
6.7k
6
votes
12
replies
1.7k
views
Error when loading DMRcate
DMRcate
14 months ago
Bazingastats
• 0
6
votes
0
replies
2.8k
views
BH vs BY in p.adjust + FDR interpretation
Coverage
limma
17.8 years ago • updated 8.3 years ago
Gordon Smyth
50k
6
votes
3
replies
2.9k
views
plotDEXSeq error in plot.window(xlim = c(0, 1), ylim = c(0, max(matr))) : need finite 'ylim' values
dexseq
differential exon usage
7.2 years ago
fizer
▴ 30
5
votes
1
reply
2.5k
views
fdrtool - correction of p-values - course found on huber.embl.de
fdrtool
p-value distribution
7.1 years ago
j.bergenstrahle
▴ 80
5
votes
3
replies
2.0k
views
ChIPQC error: "if (nrow(pv$binding) > 0) { : argument is of length zero"
chipqc
7.8 years ago
Danielle Denisko
▴ 30
5
votes
11
replies
2.8k
views
Error while installing Phyloseq using biocLite
bioclite
phyloseq
install error
5.5 years ago
khp
▴ 30
5
votes
5
replies
1.7k
views
Running NMF for single cell
NMF
scater
3.5 years ago
lirongrossmann
▴ 40
5
votes
5
replies
2.1k
views
SGE backend on Bioc AMI with Starcluster not recognising CPUs
ami
starcluster
biocparallel
sge
AWS
8.6 years ago
n.huckle
▴ 30
5
votes
13
replies
4.1k
views
Getting error
enrichplot
clusterProfiler
org.Hs.eg.db
updated 2.1 years ago by
793345316
▴ 40 • written 2.1 years ago by
beslinail
• 0
5
votes
5
replies
1.0k
views
Warning in In chromPeakSpectra: Lazy evaluation queue is not empty, results in NULL object
xcms
Spectra
updated 2.1 years ago by
Johannes Rainer
★ 2.0k • written 2.1 years ago by
e.terlova
▴ 20
5
votes
1
reply
1.4k
views
Support for new Illumina human methylation EPIC array v2
human
methylationArrayAnalysis
updated 3 months ago by
s.malik
• 0 • written 13 months ago by
jenniewoo
▴ 70
5
votes
2
replies
1.1k
views
info(header(vcf)) remembers fields not imported: bugfix?
VariantAnnotation
7.7 years ago
kevin.rue
▴ 350
5
votes
4
replies
1.4k
views
Eliminating repetitive calls to ExperimentHub
experimenthub
annotationhub
large dataset
5.9 years ago
slinker
▴ 20
5
votes
3
replies
1.4k
views
Setting gene expression thresholds for trajectory analysis using Monocle
monocle
differential gene expression
detectgenes function
Tutorial
5.2 years ago
S.Webb6
▴ 40
5
votes
6
replies
2.4k
views
Download errors during Bioconductor 3.12 update
reactome.db
Bioconductor
BiocManager
updated 3.5 years ago by
Martin Morgan
25k • written 3.5 years ago by
Michael Steinbaugh
▴ 90
5
votes
2
replies
657
views
outdated GEOmetadb.sqlite
GEOmetadb
14 months ago • updated 3 months ago
Robert Castelo
★ 3.3k
4
votes
15
replies
6.2k
views
mzR installation error: "unable to load shared object"
mzR
installation
8.8 years ago
Eugene
• 0
4
votes
9
replies
7.8k
views
Upper triangular matrix heatmap using pheatmap package
pheatmap
8.3 years ago
Deepak Tanwar
▴ 10
4
votes
4
replies
4.9k
views
FeatureCounts
counts
subread
RNASeq
featurecounts
2.5 years ago
k___
▴ 30
4
votes
4
replies
6.8k
views
what the test method for enrichGO in clusterProfiler?
clusterProfiler
2.4 years ago
xiaofeiwang18266
▴ 50
4
votes
5
replies
2.5k
views
GDCprepare - error
TCGABiolinks
GDCprepare
4.8 years ago
vm
▴ 30
4
votes
5
replies
786
views
Strand information in a GAlignmentsList object derived from strand-specific paired-end RNA-seq data
GenomicAlignments
14 months ago
Robert Castelo
★ 3.3k
4
votes
0
replies
1.7k
views
how to test for genes of interest?
Normalization
Preprocessing
limma
Normalization
Preprocessing
limma
15.8 years ago
Gordon Smyth
50k
4
votes
6
replies
1.5k
views
HTSeq command option for single-end reads
HTSeqGenie
20 months ago
Maryam.sed1400
• 0
4
votes
3
replies
3.8k
views
subsetting GRanges object based on gene IDs
genomicranges
genomicfeatures
granges
subsetting
6.6 years ago
Assa Yeroslaviz
★ 1.5k
4
votes
4
replies
847
views
visualization problem with BioMM package
BioMM
3.8 years ago
parra.sev
▴ 20
4
votes
2
replies
980
views
BayesSpace parameters for DLPFC Visium data
BayesSpace
3.0 years ago • updated 2.9 years ago
aspangl1
▴ 30
4
votes
2
replies
645
views
A way to find which genome builds a package supports
build
genome
13 months ago
Dmitry
• 0
4
votes
7
replies
2.0k
views
How to normalize long-read RNA-seq data for comparison with short-reads
NanoporeRNASeq
LongRead
Normalization
ShortRead
IlluminaRNASeq
15 months ago • updated 14 months ago
Bernardo
▴ 10
4
votes
5
replies
1.7k
views
ChIPpeakAnno getAnnotation request fail
chippeakanno
biomart
ensembl
8.3 years ago
igor
▴ 40
4
votes
2
replies
3.4k
views
Difference between "be" and "leek" methods when deciding number of surrogate variables to estimate with SVA?
sva
num.sv
6.8 years ago
brhead
▴ 40
4
votes
6
replies
4.7k
views
How to design formula in DESeq2 when setting blind=FALSE in rlog transformation?
deseq2
normalization
9.4 years ago
Xianjun Dong
▴ 10
4
votes
0
replies
2.3k
views
sva: No significant surrogate variables
Microarray
sva
Microarray
sva
11.1 years ago
Shraddha Pai
▴ 80
1,000 results • Page
1 of 20
Recent ...
Replies
Comment: Reproducibility issue with scran together with lapply and BiocParallel
by
ATpoint
★ 4.0k
Thanks Aaron for the response. RNGseed (for me) has no effect. I actually stumbled over this in one of my analysis where I have a constant …
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
winwater0928
• 0
Thank you for your comment! 1)"Your contrast is comparing LGR5 to Homeostasis." means (Lgr5)/(Homeostasis). Is it right? Then, I think if…
Answer: Reproducibility issue with scran together with lapply and BiocParallel
by
Aaron Lun
★ 28k
IIRC **BiocParallel** switches to a different RNG when inside its own functions like `bplapply` - specifically L'Ecuyer-CMRG, as opposed to…
Comment: DESeq2 output used for PCA plot on R studio
by
swbarnes2
★ 1.3k
This looks terrible for RNASeq data, because it's not RNASeq data. It's a matrix of pretty random numbers, which you know because you poste…
Comment: DESeq2 output used for PCA plot on R studio
by
Aaliya
• 0
I had taken this code from internet, but after reading about it and I did on my own, I would be making a post to ask for the suggestions if…
Votes
C: null model and DEXSeqDataSet object in DEXSEQ
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
Answer: Interpret plot from DiffBind
Answer: How to retrieve gene ontology GO class from gene list?
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