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Bowtie
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2
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7.5k
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Multiple alignments (multi-mapped reads) and DESeq/edgeR pipeline
deseq
deseq2
edger
tophat
bowtie
8.9 years ago
ysdel
▴ 40
2
votes
4
replies
1.6k
views
Bowtie % mapped reads for DGE
Bowtie
quasr
rnaseq
updated 8.3 years ago by
Dario Strbenac
★ 1.5k • written 8.3 years ago by
tarek.mohamed
▴ 10
1
vote
4
replies
1.5k
views
Importing Bowtie result into R
r
bowtie
updated 8.9 years ago by
Sean Davis
21k • written 8.9 years ago by
a.afshinfard
▴ 10
0
votes
1
reply
806
views
Is possible to use NOISeq with counts from RSubread?
noiseq
rsubread
tophat
bowtie
bowtie2
4.9 years ago
Marcelo Laia
▴ 450
4 results • Page
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Comment: Reproducibility issue with scran together with lapply and BiocParallel
by
ATpoint
★ 4.0k
Thanks Aaron for the response. RNGseed (for me) has no effect. I actually stumbled over this in one of my analysis where I have a constant …
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
winwater0928
• 0
Thank you for your comment! 1)"Your contrast is comparing LGR5 to Homeostasis." means (Lgr5)/(Homeostasis). Is it right? Then, I think if…
Answer: Reproducibility issue with scran together with lapply and BiocParallel
by
Aaron Lun
★ 28k
IIRC **BiocParallel** switches to a different RNG when inside its own functions like `bplapply` - specifically L'Ecuyer-CMRG, as opposed to…
Comment: DESeq2 output used for PCA plot on R studio
by
swbarnes2
★ 1.3k
This looks terrible for RNASeq data, because it's not RNASeq data. It's a matrix of pretty random numbers, which you know because you poste…
Comment: DESeq2 output used for PCA plot on R studio
by
Aaliya
• 0
I had taken this code from internet, but after reading about it and I did on my own, I would be making a post to ask for the suggestions if…
Votes
C: null model and DEXSeqDataSet object in DEXSEQ
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
Answer: Interpret plot from DiffBind
Answer: How to retrieve gene ontology GO class from gene list?
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