Bioconductor

No posts found.

0 results • Page 1 of 1

Recent ...

Replies

Comment: Batch correction and repeated measures by Gordon Smyth 48k

I mean add the counts, for example by `sumTechReps`. Technical RNA-seq replicates are always combined by summing rather than by "averaging"…

Comment: Batch correction and repeated measures by Jonathan • 0

Thanks, I'll try that. BTW, what do you mean by "sum them"? Do you mean averaging them?

Comment: Batch correction and repeated measures by Gordon Smyth 48k

I see no evidence of a batch effect. I would be tempted to sum the technical replicates and analyse the data without batch correction.

Comment: Batch correction and repeated measures by Jonathan • 0

The technical replicates do look rather similar on the MDS plot, but the axes percentages are rather low... is that enough to draw conclusi…

Comment: Batch correction and repeated measures by Gordon Smyth 48k

Have you checked from the MDS plot that there is a visible batch effect for the repeated samples? Batch correction has lots of negative con…

Votes

Answer: Batch correction and repeated measures

Awards • All

Popular Question to Michael Love 40k

Locations • All

United Kingdom, 7 minutes ago

Seattle, WA, United States, 14 minutes ago

WEHI, Melbourne, Australia, 20 minutes ago

Germany, 23 minutes ago

Sweden, 33 minutes ago

Traffic: 448 users visited in the last hour

Content Search
Users
Tags
Badges

Help About
FAQ

Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the

version 2.3.6