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DESeq2
•
reset
4
votes
5
replies
911
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 1 day ago by
Esperan
• 0 • written 15 days ago by
Elijah
• 0
1
vote
5
replies
3.9k
views
DESeq2 swap ensembl gene id rownames with gene symbol column
deseq2
dataframe
ensembl gene id
gene symbol
entrezid
updated 6 days ago by
Erika
• 0 • written 5.3 years ago by
jason.williams
▴ 10
3
votes
7
replies
2.0k
views
Vertical line at A=0, nothing else, on MA plot
deseq2
updated 21 days ago by
melissa
▴ 10 • written 6.0 years ago by
david.wheeler7
▴ 20
1
vote
1
reply
283
views
DEseq2 design for additive treatment
DESeq2
updated 24 days ago by
ATpoint
★ 4.7k • written 25 days ago by
picasa1983
• 0
1
vote
1
reply
451
views
Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 24 days ago by
swbarnes2
★ 1.4k • written 24 days ago by
Len
▴ 10
2
votes
2
replies
505
views
Plot title for PCA in DESeq2
DESeq2
27 days ago • updated 23 days ago
AL
• 0
0
votes
3
replies
447
views
displaying the differential expression with deseq2 over 4X2 samples with the individual changes in gene expression per sample
DESeq2
updated 23 days ago by
Leonard
• 0 • written 6 weeks ago by
Martijn
• 0
0
votes
1
reply
323
views
How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData
DESeq2
pseudobulk
sing
updated 23 days ago by
James W. MacDonald
68k • written 23 days ago by
Sara
• 0
2
votes
5
replies
980
views
Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
22 days ago • updated 21 days ago
picasa1983
• 0
2
votes
6
replies
1.5k
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 19 days ago by
Jane
• 0 • written 10 months ago by
Reza
• 0
0
votes
3
replies
595
views
how many significant figures are there in DESeq2 output
DESeq2
updated 18 days ago by
ATpoint
★ 4.7k • written 20 days ago by
Matthew McCormack
▴ 180
0
votes
1
reply
174
views
DESeq use in lipidomic analysis
DESeq2
package
Lipidomics
updated 14 days ago by
Michael Love
43k • written 15 days ago by
泓宇
• 0
5
votes
3
replies
27k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
updated 12 days ago by
California Business Journal
• 0 • written 6.2 years ago by
aishu.jp
▴ 30
1
vote
3
replies
4.5k
views
Log2 fold change value in DESeq2
deseq2
rnaseq
updated 12 days ago by
California Business Journal
• 0 • written 7.4 years ago by
AP
• 0
1
vote
6
replies
1.8k
views
how to analysis htseq-count
deseq2
updated 12 days ago by
California Business Journal
• 0 • written 7.2 years ago by
Bioinformatics
▴ 30
1
vote
2
replies
1.2k
views
[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw
ChIPseq
DESeq2
DiffBind
9 days ago • updated 8 days ago
Luca
• 0
1
vote
3
replies
1.5k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 7 days ago by
Robert
• 0 • written 2.8 years ago by
Victor
▴ 10
0
votes
1
reply
391
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
updated 6 days ago by
James W. MacDonald
68k • written 6 days ago by
Erika
• 0
1
vote
3
replies
185
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
updated 12 hours ago by
James W. MacDonald
68k • written 18 hours ago by
Lennie
• 0
0
votes
3
replies
545
views
Differential variance analysis - can i use deseq2
DESeq2
DifferentialExpression
RNASeq
RNASeqData
scRNAseq
updated 27 days ago by
Michael Love
43k • written 4 weeks ago by
Marwan
• 0
0
votes
6
replies
2.0k
views
DESeq2 It is correct to use interaction term for patient variability?
deseq2
design
interaction term
R
written 7.1 years ago by
briverog
• 0
0
votes
4
replies
362
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 3 days ago by
questionanswer
• 0
3
votes
4
replies
2.2k
views
How to formulate 2 factor nested design in DESeq2
nested_design
RNASeqData
DESeq2
updated 29 days ago by
Mariane
• 0 • written 2.8 years ago by
JK Kim
▴ 20
5
votes
28
replies
4.7k
views
DESeq2 model - How to test case/control time course model when taking account within sample variability?
deseq2
multiple time points
time course
casecontrol
model design
updated 2 days ago by
wuwenhui2
• 0 • written 7.7 years ago by
heikki.sarin
▴ 10
0
votes
2
replies
370
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 3 days ago by
Michael Love
43k • written 11 days ago by
rohitsatyam102
▴ 20
38
votes
22
replies
17k
views
DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
deseq2
updated 8 days ago by
aitor
• 0 • written 10.5 years ago by
Michael Love
43k
26 results • Page
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Replies
Comment: DESEQ2 looking at main effects while controlling for a continuous variable
by
James W. MacDonald
68k
The covariate is accounted for when you fit the model, so you don't need to do anything with it at the contrast step. You don't need a con…
Comment: DESEQ2 looking at main effects while controlling for a continuous variable
by
Lennie
• 0
Thank you so much for taking the time and for the detailed explanation! I was wondering, in your numeric contrast of c(1, -1, 0,0,0), doe…
Answer: DESEQ2 looking at main effects while controlling for a continuous variable
by
James W. MacDonald
68k
If you use a factor effects model (with an intercept), then you will have a baseline group (the intercept), and all other coefficients exce…
Comment: Issue with DECIPHER::IdTaxa
by
Daniel
• 0
The issue is still there and it is a real pain. ape and ade4 are used by packages like Phyloseq. There should be a way to avoid these inc…
Comment: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
by
Gordon Smyth
52k
I generally recommend `block=individual` for unbalanced designs, to recover information from incomplete blocks. I would only be disuaded fr…
Votes
Answer: DESEQ2 looking at main effects while controlling for a continuous variable
Use of clusterProfiler : Error in testForValidKeytype(x, keytype)
Comment: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
Answer: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
Answer: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
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