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DESeq2
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4
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5
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911
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Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 1 day ago by
Esperan
• 0 • written 15 days ago by
Elijah
• 0
1
vote
5
replies
3.9k
views
DESeq2 swap ensembl gene id rownames with gene symbol column
deseq2
dataframe
ensembl gene id
gene symbol
entrezid
updated 6 days ago by
Erika
• 0 • written 5.3 years ago by
jason.williams
▴ 10
5
votes
28
replies
4.7k
views
DESeq2 model - How to test case/control time course model when taking account within sample variability?
deseq2
multiple time points
time course
casecontrol
model design
updated 2 days ago by
wuwenhui2
• 0 • written 7.7 years ago by
heikki.sarin
▴ 10
0
votes
1
reply
391
views
Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2
org.Rn.eg.db
updated 6 days ago by
James W. MacDonald
68k • written 6 days ago by
Erika
• 0
1
vote
3
replies
183
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
updated 12 hours ago by
James W. MacDonald
68k • written 18 hours ago by
Lennie
• 0
0
votes
4
replies
362
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 3 days ago by
questionanswer
• 0
0
votes
2
replies
370
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 3 days ago by
Michael Love
43k • written 11 days ago by
rohitsatyam102
▴ 20
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Comment: DESEQ2 looking at main effects while controlling for a continuous variable
by
James W. MacDonald
68k
The covariate is accounted for when you fit the model, so you don't need to do anything with it at the contrast step. You don't need a con…
Comment: DESEQ2 looking at main effects while controlling for a continuous variable
by
Lennie
• 0
Thank you so much for taking the time and for the detailed explanation! I was wondering, in your numeric contrast of c(1, -1, 0,0,0), doe…
Answer: DESEQ2 looking at main effects while controlling for a continuous variable
by
James W. MacDonald
68k
If you use a factor effects model (with an intercept), then you will have a baseline group (the intercept), and all other coefficients exce…
Comment: Issue with DECIPHER::IdTaxa
by
Daniel
• 0
The issue is still there and it is a real pain. ape and ade4 are used by packages like Phyloseq. There should be a way to avoid these inc…
Comment: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
by
Gordon Smyth
52k
I generally recommend `block=individual` for unbalanced designs, to recover information from incomplete blocks. I would only be disuaded fr…
Votes
Answer: DESEQ2 looking at main effects while controlling for a continuous variable
Use of clusterProfiler : Error in testForValidKeytype(x, keytype)
Comment: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
Answer: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
Answer: Using edgeR::voomLmFit for the analysis of paired clinical RNA-seq samples with
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