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DESeq2
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38
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23
replies
17k
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DESeq2 testing ratio of ratios (RIP-Seq, CLIP-Seq, ribosomal profiling)
deseq2
10.5 years ago • updated 1 day ago
Michael Love
43k
5
votes
29
replies
5.0k
views
DESeq2 model - How to test case/control time course model when taking account within sample variability?
deseq2
multiple time points
time course
casecontrol
model design
updated 1 day ago by
Michael Love
43k • written 7.7 years ago by
heikki.sarin
▴ 10
2
votes
4
replies
1.6k
views
deseq2 lfc shrinkage with few DEGs
RNASeq
DESeq2
updated 1 day ago by
Michael Love
43k • written 2.8 years ago by
Victor
▴ 10
4
votes
5
replies
918
views
Surprising behavior for DEXseq dispersion estimate plot?
DESeq2
DEXSeq
updated 3 days ago by
Esperan
• 0 • written 16 days ago by
Elijah
• 0
0
votes
4
replies
385
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2
TechnicalReplicates
RNASeq
written 4 days ago by
questionanswer
• 0
0
votes
2
replies
380
views
Removing UTR from GTF for gene quantification
edgeR
DESeq2
updated 4 days ago by
Michael Love
43k • written 13 days ago by
rohitsatyam102
▴ 20
1
vote
3
replies
267
views
DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
updated 1 day ago by
James W. MacDonald
68k • written 2 days ago by
Lennie
• 0
7 results • Page
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Comment: DiffBind custom normalization factor
by
Abbie
• 0
Hello! Thanks so much for adding dba.normalize and all the documentation in the manual! I'm hoping I can ask a follow-up question here. I…
Answer: CpGs with genomic positions greater than the chromosome size in EPICv2?
by
James W. MacDonald
68k
All of the annotation packages provided by Bioconductor are simply repackaging of existing data from one or more sources. In this case, the…
Comment: Using TPM from tximport of Salmon output for singscore
by
Kent
• 0
Thank you for the suggestions Michael. Would you say that after filtering out the low count genes, `rlog(counts, blind = T)` is doing the s…
Comment: Using TPM from tximport of Salmon output for singscore
by
Michael Love
43k
For clustering generally we recommend `vst()` see the workflow for example. For working with individual samples, I can see how a scaling/t…
Comment: DESeq2 model - How to test case/control time course model when taking account wi
by
Michael Love
43k
I'd recommend working with a local statistician or someone familiar with linear models in R. I have to reserve my time on the support site …
Votes
clusterProfiler-GO enrichment Error
A: Machine learning in R book
Data preparation and Machine learning on time course RNAseq data
Data preparation and Machine learning on time course RNAseq data
Answer: Use of org.Rn.eg.db with GRCr8 reference genome
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