Showing : DESeq2 • reset
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Discrepancies in normalised count data vs unnormalised
DESeq2
3 hours ago AMgroup • 0
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2
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249
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Design formula for circadian time-course data for vst() function
DESeq2
1 day ago • updated 23 hours ago Vishnu • 0
2
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3
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734
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Filtering after DESeq
DESeq2 IHW
5 days ago • updated 1 day ago caroline.zanchi • 0
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2
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422
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Analyzing TnSeq data with DESeq2
DESeq2
updated 23 hours ago by • 0 • written 6 days ago by • 0
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646
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How do I set up my DESeq analysis when I know a few vehicle control samples provide background effects we don't want to see in any of the subsequent …
DESeq2 RUVSeq
updated 8 days ago by 43k • written 5 weeks ago by • 0
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2
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461
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Differing results with DESeq2
DESeq2
updated 6 days ago by • 0 • written 26 days ago by • 0
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2
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320
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RNA Seq using Lrt
DESeq2
8 days ago • updated 7 days ago Rob • 0
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3
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726
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Log2foldchange strange behavior
DESeq2
updated 16 days ago by ★ 4.8k • written 16 days ago by • 0
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1
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548
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WGCNA
WGCNA DESeq2
updated 18 days ago by ★ 4.8k • written 22 days ago by • 0
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1
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835
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Comparison of results obtained from a subset of the data versus the complete dataset for a few selected genes.
DESeq2
updated 18 days ago by ★ 1.4k • written 19 days ago by ▴ 20
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1.2k
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PhD scholar
RNASeqAnalysis DESeq2
updated 19 days ago by 4.1k • written 19 days ago by • 0
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292
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DESeq2 - am I extracting results incorrectly?
DESeq2
updated 22 days ago by 68k • written 22 days ago by ▴ 30
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792
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DESeq - help with pair wise comparisons
DESeq2
23 days ago bd2000 ▴ 30
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2.5k
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DESeq: Gene is not flagged as differentially expressed despite count data showing differences
DifferentialExpression DESeq2
26 days ago • updated 23 days ago ktz • 0
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569
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DESeq2
org.Mmu.eg.db DESeq2 TimeCourse DMSOvsdrug RNASeqData
updated 4 weeks ago by ★ 4.8k • written 4 weeks ago by • 0
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6
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1.2k
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Surprising behavior for DEXseq dispersion estimate plot?
DESeq2 DEXSeq
updated 4 weeks ago by ★ 4.8k • written 7 weeks ago by • 0
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464
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DESEQ2 looking at main effects while controlling for a continuous variable
DESeq2
5 weeks ago Lennie • 0
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4
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646
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Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
DESeq2 TechnicalReplicates RNASeq
written 6 weeks ago by • 0
1
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1
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808
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Use of org.Rn.eg.db with GRCr8 reference genome
DESeq2 org.Rn.eg.db
updated 6 weeks ago by 68k • written 6 weeks ago by • 0
1
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8
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2.3k
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[ChIP-seq] DESeq2 on counts matrix from union set of called peaks
csaw ChIPseq DESeq2 DiffBind
7 weeks ago • updated 20 days ago Luca • 0
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2
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474
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Removing UTR from GTF for gene quantification
edgeR DESeq2
updated 6 weeks ago by 43k • written 7 weeks ago by ▴ 20
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1
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236
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DESeq use in lipidomic analysis
DESeq2 package Lipidomics
updated 7 weeks ago by 43k • written 7 weeks ago by • 0
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3
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683
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how many significant figures are there in DESeq2 output
DESeq2
updated 8 weeks ago by ★ 4.8k • written 8 weeks ago by ▴ 180
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5
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1.1k
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Group-specific condition effects with individuals nested: matrix is not full rank
DESeq2
9 weeks ago • updated 8 weeks ago picasa1983 • 0
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7
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2.1k
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Vertical line at A=0, nothing else, on MA plot
deseq2
updated 8 weeks ago by ▴ 10 • written 6.1 years ago by ▴ 20
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1
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488
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How to adjust for Cell Type Differences between groups in Pseudo-Bulk Differential Gene Expression analysis in scRNA-seq?
SingleCellData DESeq2 pseudobulk sing
updated 9 weeks ago by 68k • written 9 weeks ago by • 0
1
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1
reply
506
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Interpreting DESEQ2 interaction term and inclusion of covariate
DESeq2
updated 9 weeks ago by ★ 1.4k • written 9 weeks ago by ▴ 10
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1
reply
322
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DEseq2 design for additive treatment
DESeq2
updated 9 weeks ago by ★ 4.8k • written 9 weeks ago by • 0
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2
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619
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Plot title for PCA in DESeq2
DESeq2
9 weeks ago AL • 0
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3
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628
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Differential variance analysis - can i use deseq2
DESeq2 DifferentialExpression RNASeq RNASeqData scRNAseq
updated 9 weeks ago by 43k • written 10 weeks ago by • 0
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2
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1.1k
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Weird p-values using DESeq2?
DESeq2 RNASeq
updated 10 weeks ago by ★ 4.8k • written 10 weeks ago by • 0
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1
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198
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Comparing Mock vs Infected in WT and Knockout Cells
DESeq2 DifferentialExpression DifferentialRegulation RNASeq GeneSetEnrichment
updated 11 weeks ago by 43k • written 11 weeks ago by • 0
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3
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582
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DESeq2 adujsted p-value cutoff seemingly not working
DESeq2
updated 11 weeks ago by • 0 • written 11 weeks ago by • 0
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2
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432
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accounting for vs. removing batch effects with DESeq2 in RNAseq analysis
DESeq2
updated 11 weeks ago by • 0 • written 12 weeks ago by • 0
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3
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493
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displaying the differential expression with deseq2 over 4X2 samples with the individual changes in gene expression per sample
DESeq2
updated 9 weeks ago by • 0 • written 12 weeks ago by • 0
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3
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515
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How to compare expression levels of genes between scRNAseq and bulkRNAseq?
Seurat RNASeq DESeq2 scRNAseq
updated 12 weeks ago by • 0 • written 3 months ago by ▴ 10
0
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1
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287
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DEG analysis across multiple timepoints, treatments and tissues
DifferentialExpression RNASeq DESeq2
updated 3 months ago by 43k • written 3 months ago by • 0
2
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2
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515
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Selection of correct coefficients or contrasts in DESeq2
DESeq2
updated 3 months ago by ★ 1.4k • written 3 months ago by • 0
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4
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1.0k
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Why select top highly expressed genes based on normalized counts, but then plot VST-transformed values in DESeq2 pheatmap?
RNASeq DESeq2
3 months ago Quang NN ▴ 10
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2
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871
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How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot DESeq2
3 months ago Blandine • 0
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5
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432
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Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2 DifferentialExpression RNASeqData BatchEffect
written 3 months ago by • 0
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4
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678
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How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2 GSEA clusterProfiler
3 months ago Quang NN ▴ 10
2
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5
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527
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Differential variability modelling with vst() expression
limma DESeq2
written 3 months ago by • 0
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3
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663
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DESeq2 analysis with transcript counts
Transcriptomics RNASeq DESeq2
written 3 months ago by • 0
2
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9
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1.2k
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Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2 BatchEffect
3 months ago Quang NN ▴ 10
1
vote
1
reply
568
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Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond DESeq2 rnaseqDTU
updated 3 months ago by 43k • written 3 months ago by • 0
0
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1
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251
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Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 3 months ago by ★ 1.4k • written 3 months ago by • 0
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1
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451
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Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR DESeq2
updated 3 months ago by 43k • written 4 months ago by • 0
2
votes
1
reply
458
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Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2 RNASeqData
updated 3 months ago by 43k • written 4 months ago by • 0
0
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2
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502
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DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
4 months ago Kucsera • 0
50 results • Page 1 of 1
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Replies
Comment: Cannot extract size factor from DESeq2 analysis by s.malik • 0
Dear Mike, using tximeta and following code, is this fine to extract normalized counts after `sizeFactors(dds)` gives NULL? `dds <- DESeq…
Comment: Design formula for circadian time-course data for vst() function by Vishnu • 0
Thank you so much for your reply.
Comment: Analyzing TnSeq data with DESeq2 by Tom • 0
[Doncaster Locksmith][1] delivers trusted, efficient security services tailored to your needs. Analyzing TnSeq data with DESeq2 may uncover…
Answer: Design formula for circadian time-course data for vst() function by ATpoint ★ 4.8k
This was asked before: https://support.bioconductor.org/p/9148734/#9148812 Take-home message is that influence is minor, as you see your…
Comment: Filtering after DESeq by caroline.zanchi • 0
... I did it. It does filter out quite a bit of genes : ``` keep <- filterByExpr(dds, group="host_treatment_generation",min.count = 10, mi…
Votes
A: DESeq2::sizeFactors() function does not output the sizeFactor table.
Comment: Filtering after DESeq
A: Normalized counts from DESeq2 results in similar but not equal total read count?
A: DESeq2: Different Dispersions for different sample groups
A: Contrasts & coefficients in limma
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