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DEseq
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Running DESeq on normalized data
deseq
DESeq2
updated 4 months ago by
ATpoint
★ 4.0k • written 4 months ago by
Hugo
• 0
2
votes
5
replies
5.5k
views
ERROR configuration failed for package RCurl during installation of DESeq
ERROR: configuration failed for package ‘RCurl’
DESeq
written 4.0 years ago by
krunal.24.6
▴ 10
0
votes
3
replies
826
views
Difference between DESeqDataSetFromMatrix() function and DESeq() function
DESeq
DESeq2
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
Abir.khazaal
▴ 10
1
vote
2
replies
597
views
summarizeToGene in tximeta causing vastly different results
DESeq2
deseq
txime
tximeta
updated 12 months ago by
Michael Love
41k • written 12 months ago by
akrunic
• 0
0
votes
1
reply
622
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 7 months ago by
James W. MacDonald
65k • written 7 months ago by
khaserdene99
• 0
2
votes
2
replies
497
views
Predicting missing values splines DESeq2
deseq
DESeq2
5 months ago • updated 4 months ago
georgii.vdovin
• 0
1
vote
2
replies
287
views
evaluate the variable importance on genes
DESeq2
deseq
7 weeks ago
Meme
• 0
0
votes
4
replies
614
views
lfc direction of effect flipped for ashr, apeGLM, but accurate to biological data for non-shrunken
DESeq2
apeglm
DESeq
ApeGLM
Ashr
updated 9 months ago by
Michael Love
41k • written 9 months ago by
Ndimensional
▴ 20
6
votes
11
replies
26k
views
With DESeq2 "Not full rank" Error with design ~ line + time + condition
deseq
deseq2
updated 8 months ago by
Erika
• 0 • written 9.2 years ago by
c.lieftink@nki.nl
▴ 40
0
votes
1
reply
362
views
Controling for Covariates With a Continuous Predictor Variable
Deseq
DESeq2
updated 5 months ago by
Michael Love
41k • written 5 months ago by
georgii.vdovin
• 0
10 results • Page
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Comment: FilterByExpr low counts with small sample size
by
Gordon Smyth
50k
As always, it always helps to see code. Are you running `voom` or `voomLmFit`? Did you run `normLibSizes()` in edgeR? Are you starting with…
Comment: Batch correction and repeated measures
by
Gordon Smyth
50k
In your code, it was clear that `expr.mat` was a matrix rather than a DGEList, so it has to be a count matrix. If you have a DGEList object…
Comment: Methylation Analysis experiment design using Limma
by
Gordon Smyth
50k
I don't analyse methylation arrays so cannot advise on specific limma settings for that type of data. Please follow the advice of the methy…
Comment: Light difference when using coef vs omit a group when compare 3 groups.
by
Chris
• 0
Hi Gordon, thank you so much for further guidance! I discussed this issue with doctor know about Alzheimer and they don't expect significan…
Comment: Single-Cell Data Simulation from Bulk RNA-seq
by
wichaidi
• 0
Hi - I am wondering what you decided to do. I am currently in a similar condition where I need to create a toy scRNAseq data from bulk.
Votes
Answer: How many of my genes from my gene list are in each KEGG pathway?
Comment: Light difference when using coef vs omit a group when compare 3 groups.
DESeq2 variance transformation error and plot dispersion Estimate
A: DESeq2 variance transformation error and plot dispersion Estimate
ensembl to hgnc symbols creates duplicates
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