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DEseq2
•
reset
0
votes
0
replies
11
views
rlog transformation
DESeq2
1 hour ago
ishij9717
• 0
0
votes
0
replies
8
views
ONT long-read RNA sequencing - data analysis options
DESeq2
LongRead
DEXSeq
IsoformSwitchAnalyzeR
RNASeq
1 hour ago
09191919
• 0
0
votes
0
replies
22
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
21 hours ago
lonn
• 0
0
votes
0
replies
28
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
21 hours ago • updated 19 hours ago
ussarizona
• 0
0
votes
0
replies
34
views
DESEQ2 output gives multiple groups when only 2 groups are present
DESeq2
22 hours ago
quanah.hudson
• 0
0
votes
0
replies
43
views
covariate with negative and positive values
DESeq2
1 day ago
Brynn
• 0
0
votes
3
replies
128
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 1 day ago by
Michael Love
41k • written 2 days ago by
andrebolerbarros
▴ 20
1
vote
1
reply
154
views
How to retrieve detailed regression results from deseq2
DESeq2
updated 2 days ago by
Michael Love
41k • written 7 days ago by
Weiqian
▴ 10
0
votes
3
replies
159
views
DEG with low counts
DESeq2
3 days ago • updated 1 day ago
Rajat
• 0
0
votes
1
reply
86
views
Deseq2 Rlog transformation
DESeq2
updated 3 days ago by
ATpoint
★ 4.0k • written 4 days ago by
Sahar
• 0
1
vote
2
replies
146
views
Design Matrix within group comparison more than two groups
DESeq2
5 days ago • updated 4 days ago
hemantcnaik
• 0
0
votes
1
reply
100
views
Bioinformatics student
Des
DESeq2
updated 5 days ago by
hemantcnaik
• 0 • written 5 days ago by
Sahar
• 0
0
votes
1
reply
140
views
DESeq2 gives an error and R crashes
DESeq2
7 days ago • updated 6 days ago
Shefali
• 0
0
votes
2
replies
177
views
Interaction term vs grouping variable design considerations
DESeq2
DifferentialExpression
updated 8 days ago by
Michael Love
41k • written 12 days ago by
nhaus
▴ 30
0
votes
1
reply
122
views
IgG control in RIP-seq with Deseq2
DESeq2
RIP-seq
updated 8 days ago by
Michael Love
41k • written 8 days ago by
Adelaide
• 0
0
votes
2
replies
210
views
Regressing out batch effects
DESeq2
updated 8 days ago by
Michael Love
41k • written 13 days ago by
kys91240
• 0
0
votes
2
replies
128
views
What to the log 2 fold changes mean using LRT on time course in DESeq2
DESeq2
timecourse
DifferentialExpression
updated 12 days ago by
James W. MacDonald
65k • written 12 days ago by
joshua.mannheimer
• 0
1
vote
1
reply
143
views
DESeq2 - Missing values in count matrix
DESeq2
updated 13 days ago by
James W. MacDonald
65k • written 13 days ago by
Hong Thu
• 0
1
vote
6
replies
388
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2
Normalization
updated 14 days ago by
Michael Love
41k • written 14 days ago by
RL
• 0
1
vote
1
reply
126
views
DESeq2 , filter and theta arguments in resutlts()
DESeq2
15 days ago
Layal
• 0
0
votes
2
replies
139
views
pca with VST of DESeq2 give 100% variance in PC1
DESeq2
sva
15 days ago
michelafrancesconi8
• 0
0
votes
4
replies
210
views
Abnormal Low Dispersion Gene Population in DESeq2
DESeq2
updated 15 days ago by
Michael Love
41k • written 16 days ago by
Aidan
• 0
0
votes
1
reply
130
views
New genes that did not exist in dds object suddenly showed up after degPatterns
DESeq2
updated 20 days ago by
Michael Love
41k • written 20 days ago by
Mikushi
• 0
2
votes
3
replies
309
views
PCA of different bacterial species
DESeq2
updated 21 days ago by
Michael Love
41k • written 22 days ago by
Laura
▴ 10
0
votes
1
reply
146
views
collapseReplicates dimension error
DESeq2
Dese
updated 23 days ago by
Michael Love
41k • written 26 days ago by
Léa
• 0
3
votes
2
replies
186
views
How to extract counts from a DESeq dataset ordered by a specific sample?
DESeq2
Transcriptomics
23 days ago
Anita
▴ 10
1
vote
2
replies
195
views
DESeq2 "Contrast" option
DESeq2
contrast
DifferentialExpression
27 days ago • updated 21 days ago
Chanderkant Chaudhary
• 0
0
votes
2
replies
195
views
Filtering and Reference Level for DESeq2
DESeq2
GeneFiltering
RNAseq
28 days ago • updated 27 days ago
kcarey
• 0
2
votes
3
replies
267
views
DESeq2 - Plot Nr. Genes vs Variance explained in PCA
DESeq2
updated 28 days ago by
Michael Love
41k • written 4 weeks ago by
andrebolerbarros
▴ 20
0
votes
3
replies
223
views
Help with Complex DESeq2 Design
DESeq2
updated 29 days ago by
ATpoint
★ 4.0k • written 4 weeks ago by
kelsey.beavers
• 0
1
vote
1
reply
154
views
Need help with creating and plotting temporal clustering analysis of DEGs
DESeq2
ctsGE
updated 4 weeks ago by
Michael Love
41k • written 4 weeks ago by
Emma
• 0
0
votes
1
reply
126
views
Help with DESeqDataSetFromHTSeqCount
DESeq2
RSeqAn
updated 4 weeks ago by
Michael Love
41k • written 4 weeks ago by
rahaf.nazzal9
• 0
0
votes
1
reply
157
views
lfcShrink error: the line search routine failed
apeglm
DESeq2
updated 4 weeks ago by
Michael Love
41k • written 4 weeks ago by
Yuqin
• 0
2
votes
2
replies
196
views
With DESeq2 "Not full rank" Error with design
DESeq2
DEs
4 weeks ago
feather-W
• 0
0
votes
2
replies
289
views
Missing Band on Volcano Plot from DESeq2
DESeq2
Visualization
DifferentialExpression
updated 4 weeks ago by
Michael Love
41k • written 5 weeks ago by
gglatzer
• 0
0
votes
4
replies
396
views
DESeq2: subtract effect of an isotope control before comparing to a baseline sample?
DESeq2
updated 4 weeks ago by
Michael Love
41k • written 5 weeks ago by
Jad
• 0
0
votes
7
replies
394
views
DESeq2 filtering vs edgeR filtering
DESeq2
5 weeks ago • updated 22 days ago
fr8712ca-s
• 0
0
votes
2
replies
223
views
RNA sequencing raw counts gene filtering
DESeq2
limma
RNAseq
5 weeks ago • updated 4 weeks ago
kcarey
• 0
1
vote
1
reply
198
views
i'm not getting any differentially expressed genes.
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.0k • written 5 weeks ago by
Marilyn
• 0
2
votes
1
reply
220
views
Creating data matrix in Deseq2 from tximport data invalid class SummarizedExperiment object
DESeq2
tximport
updated 5 weeks ago by
ATpoint
★ 4.0k • written 5 weeks ago by
hemantcnaik
• 0
1
vote
1
reply
190
views
Perform PCA for sample clustering using DESeq2
DESeq2
updated 5 weeks ago by
ATpoint
★ 4.0k • written 5 weeks ago by
synat.keam
• 0
0
votes
3
replies
350
views
Cross-species analysis - how to compute the mid parent value?
DESeq2
limma
edgeR
DifferentialExpression
written 10 weeks ago by
pl23
• 0
0
votes
2
replies
311
views
DESeq2: Comparing A Knockout to a Strain
DESeq2
5 weeks ago
Viktor Thiel
• 0
0
votes
1
reply
194
views
DESeq2 for pseudobulk analysis of scCITE-seq
DESeq2
scCITE-seq
updated 5 weeks ago by
ATpoint
★ 4.0k • written 5 weeks ago by
daiane.hemerichbrennan
• 0
0
votes
8
replies
362
views
DESeq and SVA for batch effect
DESeq2
BatchEffect
sva
6 weeks ago • updated 5 weeks ago
michelafrancesconi8
• 0
0
votes
1
reply
196
views
Unusually large fold-changes in Deseq2 results
DESeq2
updated 6 weeks ago by
ATpoint
★ 4.0k • written 6 weeks ago by
Sneha
• 0
1
vote
4
replies
279
views
DESeq2 Replicates
DESeq2
RNASeq
6 weeks ago
kcarey
• 0
2
votes
6
replies
307
views
Factor vs Character in design
DESeq2
6 weeks ago
James Aiden
• 0
1
vote
3
replies
298
views
Dispersion estimation step is very slow with latest DESeq2 and glmGamPoi
DESeq2
glmGamPoi
6 weeks ago
I-Hsuan Lin
▴ 10
4
votes
7
replies
824
views
weird volcano plot
DESeq2
updated 6 weeks ago by
Shuning
• 0 • written 5 months ago by
shouzheng
▴ 10
50 results • Page
1 of 1
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Comment: DESeq2 setting significant p-value
by
Dev
• 0
after running DESeq2 does it sort the value on the basis of pvalue, or anything, or does it give the data back in the input format I have …
Comment: DESeq filtering specific to contrasts
by
Carlin95
• 0
But wouldn't this result in a different gene set for each analysis stratification? How can you then compare if maybe gene x comes up in Tim…
Comment: Reading huge bismark coverage files using bbseq::read.bismark
by
jacques.imbert
• 0
Hello, Using nThread = 1L fixed the issue. Thank you for your time and help.
Comment: Error in champ.load(): The following specified files do not exist
by
Basti
▴ 760
The error stems from the Basename column, please see solution here : https://support.bioconductor.org/p/p132482/
Comment: FilterByExpr low counts with small sample size
by
Gordon Smyth
50k
As always, it helps to see code. Are you running `voom` or `voomLmFit`? Did you run `normLibSizes()` in edgeR prior to `voom`? Are you star…
Votes
Comment: Reading huge bismark coverage files using bbseq::read.bismark
Answer: Reading huge bismark coverage files using bbseq::read.bismark
Answer: Reading huge bismark coverage files using bbseq::read.bismark
Answer: How many of my genes from my gene list are in each KEGG pathway?
Comment: Light difference when using coef vs omit a group when compare 3 groups.
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