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DEseq2
•
reset
0
votes
0
replies
7
views
Selection of correct coefficients or contrasts in DESeq2
DESeq2
1 hour ago
Nathan
• 0
0
votes
1
reply
150
views
How to generate circular cnetplot and color nodes and edges in the same plot ?
cnetplot
DESeq2
updated 1 day ago by
Guido Hooiveld
★ 4.1k • written 1 day ago by
Blandine
• 0
0
votes
4
replies
170
views
Is there a way to do DEG analysis across two batches sharing one sample only?
DESeq2
DifferentialExpression
RNASeqData
BatchEffect
2 days ago
georgeyu703
• 0
0
votes
4
replies
349
views
How to sort negative log2FC or FC in RNAseq for GSEA?
DESeq2
GSEA
clusterProfiler
4 days ago • updated 2 days ago
Quang NN
▴ 10
2
votes
5
replies
293
views
Differential variability modelling with vst() expression
limma
DESeq2
written 14 days ago by
jtsy6383
• 0
0
votes
2
replies
357
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
15 days ago • updated 13 days ago
sindhuri
• 0
2
votes
9
replies
729
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
16 days ago • updated 4 days ago
Quang NN
▴ 10
1
vote
1
reply
285
views
Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond
DESeq2
rnaseqDTU
updated 16 days ago by
Michael Love
43k • written 16 days ago by
muhammadsohailraza
• 0
0
votes
1
reply
159
views
Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 17 days ago by
swbarnes2
★ 1.4k • written 17 days ago by
natalievillafranca
• 0
0
votes
1
reply
348
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 20 days ago by
Michael Love
43k • written 4 weeks ago by
Jimmy Mak
• 0
2
votes
1
reply
343
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 20 days ago by
Michael Love
43k • written 28 days ago by
KR
• 0
0
votes
2
replies
263
views
DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
26 days ago • updated 24 days ago
Kucsera
• 0
1
vote
1
reply
367
views
Can DESeq2's design compensate for sequencing experimental design shortcomings?
DESeq2
updated 5 weeks ago by
Michael Love
43k • written 6 weeks ago by
txema.heredia
• 0
1
vote
9
replies
813
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 5 weeks ago by
anna.cot.anna.cot
▴ 30
0
votes
2
replies
315
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 5 weeks ago by
ATpoint
★ 4.6k • written 6 weeks ago by
bbao
• 0
1
vote
5
replies
560
views
Deseq2 Design
RNASeq
DESeq2
updated 5 weeks ago by
James W. MacDonald
67k • written 6 weeks ago by
pm_25
• 0
0
votes
5
replies
508
views
p value is 0, padj is 0
DESeq2
6 weeks ago
Wirayatida
• 0
1
vote
2
replies
490
views
Pre-filtering TPM kallisto for ssGSEA / DESeq2
tximport
DESeq2
GSVA
6 weeks ago
georgii.vdovin
• 0
0
votes
7
replies
802
views
DESeq2: Gene is DEG in one experiment but not in the other depite similar baseMean, L2FC, ...
DESeq2
updated 6 weeks ago by
txema.heredia
• 0 • written 6 weeks ago by
fjrsa
• 0
0
votes
1
reply
282
views
Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square
DESeq2
updated 6 weeks ago by
Michael Love
43k • written 6 weeks ago by
bhandary.8590
▴ 20
0
votes
3
replies
417
views
DESeq2 - Comparing Comparisons
DESeq2
updated 6 weeks ago by
James W. MacDonald
67k • written 7 weeks ago by
sk
• 0
0
votes
4
replies
543
views
lfcShrink and alpha value
lfcShrink
DESeq2
8 weeks ago • updated 7 weeks ago
cropero
• 0
2
votes
1
reply
297
views
DESeq2- merging technical vs biological replicates
DESeq2
RNASeqData
updated 7 weeks ago by
Michael Love
43k • written 8 weeks ago by
Nikita
• 0
0
votes
2
replies
219
views
DESeq2 dispersion estimates fitting drastically different based on explanatory variable scaling
dispersionestimates
DESeq2
updated 7 weeks ago by
Michael Love
43k • written 7 weeks ago by
Sam
• 0
0
votes
2
replies
267
views
DESeq2 sizefactors counfounded with biological treatment
DESeq2
8 weeks ago
Anna
• 0
1
vote
2
replies
559
views
Deseq2 res Function
DESeq2
updated 8 weeks ago by
James W. MacDonald
67k • written 8 weeks ago by
sap275
▴ 10
0
votes
4
replies
446
views
Help with RSEM counts: do I transpose matrix?
RSEM
DESeq2
8 weeks ago
NB
• 0
0
votes
5
replies
455
views
Identical samples after deseq2 batch effect removal
DESeq2
updated 8 weeks ago by
Michael Love
43k • written 9 weeks ago by
maripane
• 0
0
votes
1
reply
257
views
DE analysis on Single cell RNA Seq time course data (using pseudotime) with 5 different genotypes
DESeq2
updated 9 weeks ago by
James W. MacDonald
67k • written 9 weeks ago by
rj1435
• 0
1
vote
2
replies
316
views
Differene between the Wald and LRT test for time-series gene expression data
DESeq2
9 weeks ago
Denise
• 0
0
votes
1
reply
171
views
eRNAs count
RNASeq
DESeq2
updated 9 weeks ago by
ATpoint
★ 4.6k • written 9 weeks ago by
Irene
• 0
0
votes
1
reply
409
views
DESeq2 rerun returns different results
DESeq2
updated 10 weeks ago by
ATpoint
★ 4.6k • written 10 weeks ago by
wl1111
• 0
0
votes
1
reply
263
views
Is my time course analysis with DESeq2 valid?
GeneExpression
DESeq2
TimeCourse
updated 10 weeks ago by
Michael Love
43k • written 10 weeks ago by
Jason
• 0
0
votes
1
reply
247
views
DESeq2 "Not full rank" and "less than full rank" error
DESeq2
deseq
updated 10 weeks ago by
Guido Hooiveld
★ 4.1k • written 10 weeks ago by
Najla Abassi
• 0
2
votes
2
replies
646
views
Handling biological replicates in DESeq2
DESeq2
updated 10 weeks ago by
ATpoint
★ 4.6k • written 10 weeks ago by
sk
• 0
5
votes
2
replies
26k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
written 6.0 years ago by
aishu.jp
▴ 30
1
vote
6
replies
1.0k
views
Volcano plot has many values around zero
RNA-seq
DESeq2
volcanoplot
updated 10 weeks ago by
Michael Love
43k • written 10 weeks ago by
nramakr
▴ 10
0
votes
2
replies
396
views
DESeq2 interaction between cancer cells and microenvironment cells
DESeq2
written 11 weeks ago by
ballackselma
• 0
4
votes
5
replies
690
views
DESeq Input from Salmon + tximport/tximeta
tximportData
summarizeToGene
tximport
DESeq2
salmon
11 weeks ago
LuciaNhu
• 0
0
votes
1
reply
421
views
Average expression of genes across replicates
DESeq2
updated 11 weeks ago by
ATpoint
★ 4.6k • written 11 weeks ago by
James
• 0
0
votes
0
replies
158
views
News:
RNAseq data analysis with R/Bioconductor (4-15 November)
DESeq2
GenomicRanges
12 weeks ago
info
▴ 10
0
votes
10
replies
1.4k
views
Question about using LRT test for time course experiments with DESeq2
DESeq2
12 weeks ago • updated 11 weeks ago
Denise
• 0
0
votes
1
reply
370
views
Deseq to output continous sequencing data
DESeq2
updated 12 weeks ago by
Michael Love
43k • written 3 months ago by
Neha
• 0
1
vote
4
replies
876
views
Adjusted p-values become NA when sub-setting samples
DESeq2
3 months ago • updated 11 weeks ago
txema.heredia
• 0
3
votes
3
replies
948
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 12 weeks ago by
Satoshi
• 0
0
votes
2
replies
381
views
ESTMATE, MCPcounter, EPIC, xCell input
DESeq2
ESTIMATE
RNASeqData
MCPcounter
written 3 months ago by
Aleksandra
• 0
0
votes
1
reply
333
views
Should zero counts in some replicates lead to inflated p-values?
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
nicolettacommins
• 0
2
votes
3
replies
1.1k
views
Analysis of large RNA-Seq Datasets
DESeq2
updated 3 months ago by
Michael Love
43k • written 3 months ago by
KokiBio
• 0
1
vote
4
replies
811
views
DESeq2 - interactions when using factors concatenated into group
DESeq2
3 months ago
boczniak767
▴ 740
0
votes
1
reply
235
views
2 factor analysis using DESeq2 but the result is not what I expected from the design
DESeq2
3 months ago
JY
• 0
50 results • Page
1 of 1
Recent ...
Replies
Answer: WGCNA sample-data preprocessing order
by
ATpoint
★ 4.6k
Hi, WGCNA is not a Bioconductor package, please read the documentation to get guidance. See https://support.bioconductor.org/p/9155561/. …
Answer: WGCNA: Can we use high power (26) for signed network?
by
ATpoint
★ 4.6k
Hi, WGCNA is not a Bioconductor package, please read the documentation to get guidance. See https://support.bioconductor.org/p/9155561/. C…
Answer: Ph.D. scholar
by
ATpoint
★ 4.6k
Hi, WGCNA is not a Bioconductor package, please read the documentation to get guidance. See https://support.bioconductor.org/p/9155561/. C…
Comment: Recount3 data correspond to TCGA data
by
Fep29
• 0
Hi, This the solution to mapping TCGA ids in Recount3 to the ids used in the TCGA barcodes: library(recount3) human_projects <- …
Answer: Unable to find sample sheet file for ChAMP methylation data analysis
by
James W. MacDonald
67k
There is an example file in the extdata directory of the `minfiData` package that you could emulate.
Votes
Answer: DESeq2- merging technical vs biological replicates
Answer: SingleCellExperiment convenience function for metadata+expression?
How to correct for tumor purity using DESeq2 to analyze TCGA tumor sample expression.
Answer: EdgeR Biological CVs
Answer: Error in download.file for TCGAutils::symbolsToRanges
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