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Showing :
EdgeR
•
reset
0
votes
4
replies
198
views
FilterByExpr low counts with small sample size
edgeR
2 days ago • updated 22 hours ago
Jonathan
▴ 10
0
votes
2
replies
59
views
How to make DGEList and design matrix by edgeR package
DGEList
edgeR
1 day ago
prity6459
• 0
0
votes
3
replies
81
views
Finding DEGs by using edgeR
subsettingdata
edgeR
designmatrix
updated 1 day ago by
Gordon Smyth
50k • written 1 day ago by
prity6459
• 0
0
votes
1
reply
72
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 1 day ago by
Gordon Smyth
50k • written 2 days ago by
Shaimaa Gamal
• 0
3
votes
3
replies
267
views
permutation test in edgeR
rna-seq
edgeR
updated 5 days ago by
Gordon Smyth
50k • written 12 days ago by
Netanel
• 0
1
vote
3
replies
311
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 5 days ago by
Gordon Smyth
50k • written 10 days ago by
Simran
• 0
5
votes
6
replies
384
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 7 days ago by
ATpoint
★ 4.0k • written 10 days ago by
nhaus
▴ 30
1
vote
3
replies
223
views
Lib sizes in the DGE object are different from original lib sizes
edgeR
DifferentialExpression
updated 14 days ago by
James W. MacDonald
65k • written 14 days ago by
Shaimaa Gamal
• 0
0
votes
2
replies
142
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
17 days ago
Marianna
▴ 20
0
votes
4
replies
387
views
Adjust RNA-seq counts (more than one batch variable) for plotting differentially expressed genes
ComBat
sva
edgeR
19 days ago • updated 17 days ago
Lucy
▴ 60
3
votes
5
replies
327
views
Differing results from plotMDS and PCAtools
edgeR
PCAtools
updated 20 days ago by
Gordon Smyth
50k • written 22 days ago by
Fabian
• 0
0
votes
6
replies
305
views
Doing comparison of RNA-seq data using EdgeR
metaMSdata
edgeR
updated 21 days ago by
Gordon Smyth
50k • written 22 days ago by
Faisal
• 0
0
votes
1
reply
208
views
Using splicing junction counts with edgeR/limma-voom
splicing
limma
edgeR
updated 29 days ago by
Gordon Smyth
50k • written 29 days ago by
scoops_streams_06
• 0
5
votes
3
replies
670
views
What benchmark should I use for setting the EdgeR filterByExpr min.count parameter?
edgeR
DifferentialExpression
updated 23 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Jack S.
▴ 50
2
votes
3
replies
273
views
edgeR, 3 x 2 design with a batch effect and contrasts
design
edgeR
contrasts
updated 4 weeks ago by
Gordon Smyth
50k • written 4 weeks ago by
JKim
• 0
0
votes
2
replies
251
views
RNAseq analysis
rnaseqGene
edgeR
DEGraph
DifferentialExpression
updated 5 weeks ago by
Gordon Smyth
50k • written 5 weeks ago by
z_bahari
• 0
2
votes
2
replies
238
views
between lane normalization upper quantile
EDASeq
edgeR
5 weeks ago • updated 4 weeks ago
elesofi
• 0
2
votes
2
replies
239
views
Question about extracting co-efficients for pairwise comparisons in edgeR
limma
fit
design
RNASeq
edgeR
5 weeks ago
Sabiha
▴ 20
0
votes
3
replies
354
views
Cross-species analysis - how to compute the mid parent value?
DESeq2
limma
edgeR
DifferentialExpression
written 11 weeks ago by
pl23
• 0
3
votes
4
replies
378
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 6 weeks ago by
Gordon Smyth
50k • written 6 weeks ago by
Jack S.
▴ 50
0
votes
1
reply
207
views
Different results from DGE using different subgroups in model.matrix
edgeR
updated 6 weeks ago by
Gordon Smyth
50k • written 6 weeks ago by
Fabian
• 0
0
votes
11
replies
677
views
Strange logFC values in differential methylation site anslysis
edgeR
MethylationArray
updated 6 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
mico
• 0
0
votes
1
reply
231
views
ExactTest vs QLFTest
edgeR
updated 7 weeks ago by
Gordon Smyth
50k • written 7 weeks ago by
fr8712ca-s
• 0
1
vote
6
replies
443
views
Library size normalization in methylation count analysis
edgeR
MethylationArray
7 weeks ago
mico
• 0
2
votes
6
replies
383
views
edgeR: DEGs in a CRD factorial (2x2x2)
edgeR
9 weeks ago • updated 8 weeks ago
tsl0026
• 0
5
votes
4
replies
366
views
Confusion about LFC using contrasts weights in EdgeR
edgeR
9 weeks ago • updated 8 weeks ago
pl23
• 0
0
votes
8
replies
594
views
Divergent results using exact, glmLRT, and glmQLF tests in edgeR
edgeR
9 weeks ago • updated 8 weeks ago
Marianna
▴ 20
3
votes
2
replies
369
views
How does one test for interactions in EdgeR?
edgeR
10 weeks ago • updated 9 weeks ago
pl23
• 0
0
votes
4
replies
318
views
Nested paired design
edgeR
10 weeks ago
manduchi
▴ 10
1
vote
2
replies
298
views
How to get normalized values for methylation counts in edgeR
edgeR
MethylationArray
10 weeks ago
mico
• 0
2
votes
3
replies
337
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 10 weeks ago by
James W. MacDonald
65k • written 10 weeks ago by
Shaimaa Gamal
• 0
4
votes
4
replies
414
views
edgeR - Design matrix for comparisons of all and subsets of samples
edgeR
updated 11 weeks ago by
James W. MacDonald
65k • written 3 months ago by
Geo
• 0
6
votes
7
replies
535
views
readDGE in edgeR giving me path names as sample names
edgeR
updated 11 weeks ago by
Gordon Smyth
50k • written 12 weeks ago by
aa.machado001
• 0
1
vote
8
replies
513
views
edgeR error "Design matrix not of full rank"
edgeR
methylation
updated 11 weeks ago by
Gordon Smyth
50k • written 12 weeks ago by
sat
• 0
0
votes
0
replies
223
views
Which normalization should I do use for ATAC Seq using DiffBind
DiffBind
edgeR
ATACSeq
DESeq2
12 weeks ago
Jawahar
• 0
2
votes
4
replies
526
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
3 months ago • updated 12 weeks ago
Sabiha
▴ 20
0
votes
3
replies
316
views
EdgeR for methylation analysis when there is no replicates
RRBSdata
edgeR
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
annasoudi
• 0
3
votes
3
replies
635
views
EdgeR: Should I include all samples/conditions in my dispersion estimation while only testing for certain contrasts
edgeR
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
Jurgen
• 0
4
votes
9
replies
1.1k
views
RNAseq paired-sample differantial expression analysis with many samples
DESeq2
edgeR
3 months ago
Luca
• 0
0
votes
1
reply
289
views
download problem
edgeR
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
theokirkland3
• 0
0
votes
5
replies
590
views
Help with model for differential expression analysis
DifferentialExpression
edgeR
3 months ago • updated 11 weeks ago
Lucy
▴ 60
1
vote
2
replies
422
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
4 months ago
Sabiha
▴ 20
5
votes
2
replies
513
views
Filter genes by expression for voomLmFit
DEG
edgeR
limma
voom
filterByExpr
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
annikagable
• 0
1
vote
2
replies
375
views
EdgeR: Exact test and glmQLFTest produce very different results
DifferentialExpression
edgeR
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
Jurgen
• 0
7
votes
2
replies
738
views
limma::voom vs edgeR::voomLmFit - when to use?
edgeR
limma
voom
RNASeq
muscat
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
annikagable
• 0
0
votes
3
replies
433
views
'Subtracting' counts on basis of Negative control media and water samples
limma
edgeR
RNASeq
DESeq2
5 months ago • updated 4 months ago
Karthik
• 0
4
votes
2
replies
572
views
DecideTest vs topTAGs in edgeR
RNAseq
StatisticalMethod
edgeR
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
alvaroperona1
▴ 10
6
votes
7
replies
909
views
[Bug] Segfault in edgeR::cpm
edgeR
segfault
updated 6 months ago by
ATpoint
★ 4.0k • written 6 months ago by
jonas.freimuth
▴ 20
0
votes
0
replies
259
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
6 months ago
Florian
• 0
1
vote
3
replies
431
views
edgeR: Time course RNA-seq experiments
edgeR
updated 6 months ago by
ATpoint
★ 4.0k • written 6 months ago by
SuperDad
• 0
50 results • Page
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Replies
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
by
Chris
• 0
Thanks Robert!
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
by
Robert Castelo
★ 3.2k
Hi, your `c2` object storing all the gene sets is probably a `list` object, where element names are the names of the pathways given by MSig…
Comment: Differential peaks that were not identified in diffbind were identified in diseq
by
lonn
• 0
I tried the default normalization and got similar results as above. Perhaps I need to try identifying the difference peak without using dif…
Answer: Cannabis and KEGGgraph
by
deeenes
▴ 30
Hello, I'm a developer of the `OmnipathR` package, and I wrote [a function][1] to access certain KEGG data: library(OmnipathR) cs…
Answer: RNAseq:Technical replicates from different batches
by
Gordon Smyth
50k
I am not entirely clear what you mean by "technical" replicates in this case. Are they resequencing of the same RNA samples or are they fre…
Votes
Comment: The difference when using Limma compare between 3 conditions vs 2 conditions
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
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