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Limma
•
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0
votes
0
replies
18
views
Testing Differential Expression with Interaction Effect Between Categorical and Continuous Variable with Repeated (Paired) Measures using variancePar…
limma
variancePartition
21 hours ago
GJ
• 0
0
votes
2
replies
325
views
Paired analysis in proteomics data for DE while interested in only a particular subset of the data
limma
modelmatrix
pairedanalysis
Proteomics
written 3 days ago by
svlachavas
▴ 840
2
votes
4
replies
210
views
Differential variability modelling with vst() expression
limma
DESeq2
updated 4 days ago by
Michael Love
43k • written 4 days ago by
jtsy6383
• 0
6
votes
5
replies
509
views
How do I fix an odd voom plot in a combined dataset?
limma
Normalization
voom
updated 6 days ago by
James W. MacDonald
67k • written 12 days ago by
1sunmic2
• 0
0
votes
2
replies
430
views
Cross-validation with multiple control subgroups in limma
limma
multtest
24 days ago • updated 23 days ago
Ali Barry
▴ 40
1
vote
9
replies
763
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 27 days ago by
anna.cot.anna.cot
▴ 30
0
votes
2
replies
295
views
Advice needed: Using limma for differential pathway analysis with continuous disease progression scores
limma
DifferentialExpression
Designmatrix
5 weeks ago • updated 4 weeks ago
Tadeoye
▴ 20
0
votes
2
replies
479
views
sva: get a sv for RIN scores
limma
sva
variancePartition
voom
RNASeq
updated 5 weeks ago by
Gordon Smyth
52k • written 5 weeks ago by
Lina
▴ 10
2
votes
3
replies
1.2k
views
How to annotate a limma Elist raw class object with an annotation file
limma
AnnotationDbi
updated 5 weeks ago by
James W. MacDonald
67k • written 5 weeks ago by
giulia.gentile
▴ 10
1
vote
2
replies
337
views
contrast matrix design for a continuous variable
limma
6 weeks ago
dtzhu337
• 0
3
votes
3
replies
620
views
Crossover design with limma
limma
crossover
updated 28 days ago by
Gordon Smyth
52k • written 6 weeks ago by
Marek Gierlinski
▴ 30
0
votes
9
replies
896
views
Differential Expression Genes as Independent Variables
limma
DifferentialExpression
variancePartition
dream
updated 5 weeks ago by
James W. MacDonald
67k • written 6 weeks ago by
abadgerw
• 0
6
votes
10
replies
2.5k
views
What type of counts data to import for performing Isoform analysis in edgeR
limma
isoform
edgeR
salmon
R
updated 5 weeks ago by
ATpoint
★ 4.6k • written 6 weeks ago by
mohammedtoufiq91
▴ 10
1
vote
3
replies
438
views
Struggle with the replicates of epic array data
limma
epicarray
IlluminaHumanMethylationEPICanno.ilm10b2.hg19
written 7 weeks ago by
Melisa Tecik
• 0
0
votes
2
replies
203
views
List of DEGs belonging to an Agilent-single color microarray experiment using Limma package
limma
8 weeks ago • updated 7 weeks ago
giulia.gentile
▴ 10
4
votes
2
replies
560
views
Using voom-transformed counts for unsupervised analyses (PCA, Random Forest, Elastic Net): Best practices?
limma
limma-voom
updated 8 weeks ago by
Gordon Smyth
52k • written 8 weeks ago by
Ahdee
▴ 50
0
votes
2
replies
197
views
Limma Interaction Effect with Covariates
limma
DMRcate
8 weeks ago
kitzhu
• 0
2
votes
3
replies
907
views
[How to construct a model.matrix ]
limma
modelmatrix
updated 8 weeks ago by
Gordon Smyth
52k • written 8 weeks ago by
kyj2226
• 0
3
votes
4
replies
662
views
Limma- Print-tip loess normalisation-printer layout information does not match M row dimension
Normalization
limma
within-arraynormalisation
Printerlayout
8 weeks ago
dseauer
• 0
1
vote
3
replies
459
views
makeContrastsDream for Interaction
limma
DifferentialExpression
variancePartition
dream
9 weeks ago • updated 6 weeks ago
abadgerw
• 0
0
votes
5
replies
671
views
Merge Replicates vs Mixed Model
limma
DifferentialExpression
variancePartition
dream
9 weeks ago • updated 6 weeks ago
abadgerw
• 0
1
vote
8
replies
1.1k
views
Combining RNAseq and microarray data using edgeR/limma-voom
limma
voom
RNASeq
edgeR
Microarray
written 10 weeks ago by
Adam Marstrand
• 0
1
vote
2
replies
958
views
Consistent Decimals In Data After Antibody Array Background Correction Using Limma:nec Function
limma
MicroarrayData
Normalization
10 weeks ago • updated 9 weeks ago
Ai
• 0
3
votes
3
replies
937
views
Robust way of dealing with low number of samples for Differential Gene Expression
limma
edgeR
DESeq2
MAST
written 11 weeks ago by
Satoshi
• 0
4
votes
3
replies
819
views
Analysis of 2*4 factorial design
limma
updated 10 weeks ago by
Gordon Smyth
52k • written 11 weeks ago by
Sudipta
• 0
2
votes
12
replies
1.7k
views
limma technical repeat correction
limma
lmer
duplicateCorrelation
technicalrepeats
11 weeks ago • updated 7 weeks ago
john
• 0
4
votes
6
replies
1.5k
views
LIMMA Differing P-Values for Treatment-Contrast Parameterization vs Group-Means Parameterization
limma
updated 11 weeks ago by
Gordon Smyth
52k • written 11 weeks ago by
abadgerw
• 0
4
votes
6
replies
1.2k
views
Zero intra-block correlation in RRBS analysis using voomLmFit with block
limma
duplicateCorrelation
edgeR
modelMatrixMeth
voomLmFit
12 weeks ago • updated 11 weeks ago
altintas.ali
• 0
2
votes
2
replies
365
views
Automatically generate contrasts from a model matrix / formula
limma
updated 3 months ago by
ATpoint
★ 4.6k • written 3 months ago by
Jonathan
▴ 10
3
votes
3
replies
340
views
How to subset voomLmFit (MArrayLM) object?
limma
edgeR
3 months ago
Guido Hooiveld
★ 4.1k
0
votes
3
replies
390
views
Need help interpreting heatmaps from limma
limma
written 3 months ago by
lhwh1208
• 0
2
votes
1
reply
1.5k
views
Removing Effect of Covariate in Only a Subset of Samples in a Model Containing All Samples Loses Full Rank if Factor but not Numerical
limma
updated 3 months ago by
James W. MacDonald
67k • written 3 months ago by
danphillips28
▴ 10
5
votes
4
replies
518
views
How to control batch effect if Group and batch are same?
limma
DESeq2
BatchEffect
updated 3 months ago by
ATpoint
★ 4.6k • written 3 months ago by
Bioinformagician
• 0
1
vote
2
replies
502
views
Poor dispersion fit in DESeq2
limma
DESeq2
RNASeq
edgeR
updated 3 months ago by
Michael Love
43k • written 3 months ago by
Ben
▴ 10
0
votes
0
replies
249
views
Assistance with Bulk RNA-seq Data Analysis Including Random Effect (`duplicateCorrelation` from `limma`)
limma
RNAseq123
3 months ago
tim.meese
• 0
0
votes
2
replies
411
views
EdgeR - Apparent Wrong Direction of logFC with Low Count Data
limma
edgeR
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
blmurphy
• 0
0
votes
2
replies
489
views
Question on performing ANOVA-like analysis using limma
limma
updated 3 months ago by
Gordon Smyth
52k • written 3 months ago by
grubble
• 0
17
votes
9
replies
7.2k
views
How to use removeBatchEffect for removing effect of multiple confounding variables
limma
removebatcheffect
deseq2
updated 3 months ago by
James W. MacDonald
67k • written 8.6 years ago by
ompandey
▴ 10
2
votes
1
reply
381
views
Question: How to Set Up Contrasts for Case-Control Analysis in Limma While Adjusting for Covariates?
MethylationArray
EpigeneticsWorkflow
limma
Regression
updated 4 months ago by
James W. MacDonald
67k • written 4 months ago by
rae.alshammari
▴ 10
0
votes
1
reply
725
views
Unable to install/use limma
limma
Bioconductor
updated 4 months ago by
James W. MacDonald
67k • written 4 months ago by
Jennifer
• 0
3
votes
3
replies
564
views
voom does not seem to properly correct for library size
limma
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
ATpoint
★ 4.6k
1
vote
5
replies
858
views
RNASeq normlization for samples with global shift in gene expression
qsmooth
DESeq2
limma
Normalization
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Xuan Yi
• 0
0
votes
3
replies
588
views
Differential expression analysis in time course experiments with DESeq2
DESeq2
limma
DifferentialExpression
updated 4 months ago by
Gordon Smyth
52k • written 4 months ago by
Jay
• 0
3
votes
4
replies
662
views
using limma trend workflow in CAMERA
CAMERA
limma
5 months ago • updated 4 months ago
Jay
• 0
3
votes
3
replies
602
views
One-sided tests with CAMERA-PR
CAMERA
limma
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Tyler Sagendorf
▴ 10
1
vote
6
replies
737
views
limma and Syntactically Invalid Factor Values
limma
updated 5 months ago by
Gordon Smyth
52k • written 5 months ago by
Dario Strbenac
★ 1.5k
1
vote
1
reply
624
views
DEG Analysis using Limma on DSP GeoMx Data: Handling Multiple ROIs per Core
limma
DSP
GeomxTools
5 months ago
Bioinformagician
• 0
4
votes
3
replies
614
views
Limma comparing gene expression between base line and one treatment
limma
updated 5 months ago by
James W. MacDonald
67k • written 6 months ago by
AL
• 0
0
votes
1
reply
581
views
How to rank DEG for GSEA using DEseq2 result ?
DESeq2
limma
GeneSetEnrichment
updated 6 months ago by
ATpoint
★ 4.6k • written 6 months ago by
picasa1983
• 0
2
votes
5
replies
666
views
Limma - 0 downreg and upreg values
GeoMx
limma
DifferentialExpression
updated 5 months ago by
James W. MacDonald
67k • written 6 months ago by
sop
• 0
50 results • Page
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Answer: promoters_txdb <- promoters(txdb, upstream = 2000) is not 2000
by
James W. MacDonald
67k
If you use `promoters` and do not provide an argument for something that has a default, you will get the default argument. Your last call t…
Comment: org.Hs.eg.db issue
by
k.panov
• 0
installed R on another PC, works perfectly. ergo, problem outside of R probably..... :-(
Comment: MBDseq analysis using qsea package
by
Lukas
▴ 20
Hi, I checked in with Matthias and here is his response: You need to indicate "samples": "The indices of the samples for which individu…
Comment: DESeq2 analysis with transcript counts
by
sindhuri
• 0
Thank you for the response. Currently I am using the R studio for analysis and Im running DESeq2 for analysis of the **transcript counts.ts…
Comment: iam not able to do retrive data using GEOquery R package
by
James W. MacDonald
67k
Another alternative is to use the `GEOmetadb` package ([vignette here)][1], which will allow you to make queries to identify GEO datasets t…
Votes
A: Linear regression in DESeq2
Answer: Is there any chance to solve "the model matrix is not full rank" in DESeq2?
Answer: Issue carrying out DiffBind Analysis at a predefined peak set
Answer: Differential variability modelling with vst() expression
Answer: Differential variability modelling with vst() expression
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