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Showing :
RNA-SEQ
•
reset
41
votes
44
replies
11k
views
Are published RNA seq data analyses often wrong in calculating p-values and FDR?
FDR
p-value
DESeq
RNA-Seq
updated 6.8 years ago by
Wade Davis
▴ 60 • written 7.4 years ago by
yjiangnan
▴ 10
10
votes
14
replies
9.1k
views
too many genes with low read-counts as differential expressed in DESeq2
deseq2
rna-seq
8.2 years ago • updated 8.1 years ago
anand_mt
▴ 90
1
vote
14
replies
11k
views
DESeq Error in round(assay(se)) : non-numeric argument to mathematical function
deseq
rna-seq
9.6 years ago
rodriguez.varenka
• 0
1
vote
13
replies
6.5k
views
"Over-correction" in the size-factors of the DESeq2 package
deseq2
rna-seq
normalization
8.5 years ago
gil.hornung
• 0
0
votes
13
replies
2.1k
views
Understanding differences in results with and without setting of pairing factor
DESeq2
deseq2
rna-seq
paired samples
updated 7.5 years ago by
Michael Love
42k • written 7.5 years ago by
solgakar@bi.technion.ac.il
▴ 90
1
vote
12
replies
1.6k
views
RNA-Seq and edgeR: remarkable differences between cpms and fitted values
RNA-Seq
edger
fitted values
differential expression
normalization
updated 5.3 years ago by
Gordon Smyth
51k • written 5.3 years ago by
David R
▴ 90
18
votes
12
replies
4.0k
views
Low-count filtering with uneven sequencing depth between samples?
edgeR
deseq2
rna-seq
independent filtering
updated 9.3 years ago by
Michael Love
42k • written 9.3 years ago by
Ryan C. Thompson
★ 7.9k
5
votes
12
replies
6.0k
views
DESeq2 time series contrasts between timepoints
rna-seq
deseq2
updated 3.0 years ago by
AZ
▴ 30 • written 7.0 years ago by
gtho123
▴ 40
9
votes
12
replies
8.4k
views
Multi factor design edgeR/Deseq2
deseq2
edgeR
rna-seq
updated 5 months ago by
Elizabeth
• 0 • written 9.3 years ago by
gthm
▴ 30
15
votes
12
replies
2.8k
views
ZINB-WaVE + DESeq2 misses low-expressed, single-condition genes that look like they should be markers
deseq2
ZINB-WaVE
single cell
RNA-seq
differential expression
updated 6.2 years ago by
Michael Love
42k • written 6.2 years ago by
rory.kirchner
▴ 60
3
votes
12
replies
5.3k
views
Batch correction when only some subjects have replicates across batches
limma
batch effect
rna-seq
updated 6.0 years ago by
Peter Langfelder
★ 3.0k • written 6.0 years ago by
Mike A
▴ 10
4
votes
11
replies
2.6k
views
Enquiry about the summarizeOverlaps() function
summarizeoverlaps
summarizedexperiment
count matrix
low counts
rna-seq
updated 8.7 years ago by
Valerie Obenchain
★ 6.8k • written 8.7 years ago by
shania90.lk
▴ 10
24
votes
11
replies
19k
views
when to apply quantile normalization with voom in limma/voom framework with RNA-seq data?
limma voom
limma
voom
rna-seq
quantile normalization
updated 8.7 years ago by
Gordon Smyth
51k • written 8.7 years ago by
lassancejm
▴ 50
9
votes
11
replies
5.8k
views
Using 'tximport' library for downstream DGE after quantifying with Kallisto
tximport
Kallisto
rna-seq
updated 7.6 years ago by
pablo_garcia05
▴ 20 • written 8.5 years ago by
cguzman.bioinformatics
▴ 10
0
votes
11
replies
1.8k
views
Issue during transcript variant analysis using rnaseqDTU-DRIMseq-DEXseq.
rnaseqDTU
DRIMseq
DEXseq
rna-seq
5.5 years ago
saddamhusain77
• 0
2
votes
11
replies
3.4k
views
Cannot find header.json in salmon index directory
salmon
tximeta
rna-seq
4.9 years ago • updated 4.8 years ago
mico
• 0
5
votes
11
replies
3.7k
views
DESeq2 logfoldchange and interaction in model
deseq2
R
logfoldchange
rna-seq
5.4 years ago • updated 5.2 years ago
pierre.marin
• 0
3
votes
11
replies
2.1k
views
normalization methods for scRNA-seq and RNA-seq in specific cases of increased global transcription
RNA-seq
scRNA-seq
updated 4.7 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
Bogdan
▴ 670
2
votes
10
replies
2.0k
views
Coseq error with DESeq2 object: Error in "normFactors"
coseq
rna-seq
updated 5.6 years ago by
andrea.rau
▴ 80 • written 5.6 years ago by
varunorama
• 0
0
votes
10
replies
2.6k
views
Error in reading data on isoformswitchanalyzeR
isoformswitchanalyzeR
rna-seq
splice-variants
written 5.5 years ago by
saddamhusain77
• 0
12
votes
10
replies
7.5k
views
Pre-processing RNA-seq data (normalization and transformation) for GSVA
gsva
gene set variation analysis
normalization
transformation
rna-seq
6.5 years ago • updated 5.7 years ago
rached
▴ 60
2
votes
10
replies
10k
views
adjusted p-values and p-values problems!
pvalue
adjusted pvalue
r
bonferroni
rna-seq
updated 8.0 years ago by
Michael Love
42k • written 8.0 years ago by
fromhj304
▴ 10
2
votes
10
replies
2.3k
views
Design matrix for unbalanced data
edgeR
RNA-Seq
design matrix
multifactorial design
8.6 years ago
Yogender Gowtham
▴ 20
2
votes
9
replies
2.3k
views
i can't find cummeRbund package
software error
cuumeRbund
rna-seq
updated 9.1 years ago by
Martin Morgan
25k • written 9.1 years ago by
Angel
▴ 40
1
vote
9
replies
2.2k
views
Difference between gene-level test and transcript-level test with RATs (Relative Abundance of Transcrits)
RATs
RNA-Seq
Isoforms
DTU
expression
updated 5.3 years ago by
fruce_ki
▴ 20 • written 5.3 years ago by
UserAnonyme
• 0
14
votes
9
replies
16k
views
How does DESeq2 handle zero counts in one condition?
deseq2
deseq
rna-seq
9.8 years ago
gavrielmatt
▴ 30
5
votes
9
replies
2.2k
views
GSVA perfomed on RSEM Expected Count data
GSVA
RNA-Seq
RSEM
Expected_Count
2.2 years ago
Yang Shi
▴ 10
3
votes
9
replies
2.6k
views
summarizeToGene from tximeta: not all 'rnames' found or unique.
RNA-seq
tximeta
3.8 years ago
Pufall, Miles A
▴ 30
10
votes
9
replies
2.0k
views
Is there are better way to improve the design via blocking or contrasts while using limma-voom for the mentioned datasets?
limma-voom
voom
limma
rna-seq
updated 7.0 years ago by
Aaron Lun
★ 28k • written 7.0 years ago by
vd4mmind
• 0
4
votes
9
replies
1.6k
views
RNA-seq analysis edgeR
rna-seq
edger
updated 7.9 years ago by
Ryan C. Thompson
★ 7.9k • written 7.9 years ago by
es874
▴ 20
14
votes
9
replies
4.1k
views
Is Limma's removeBatchEffect() and log2() commutative?
limma
rna-seq
removebatcheffect()
updated 6.7 years ago by
alakatos
▴ 130 • written 8.5 years ago by
Ekarl2
▴ 80
2
votes
8
replies
1.9k
views
Transcript variant analysis on Salmon output
Salmon
RNA-Seq
Transcript variant analysis
updated 5.5 years ago by
Michael Love
42k • written 5.5 years ago by
saddamhusain77
• 0
1
vote
8
replies
3.4k
views
RNA-seq bacterial transcriptome analysis in EdgeR with unusual dispersion trend
RNA-seq
edgeR
estimatedispersions
bacteria
9.7 years ago
Mauve
• 0
11
votes
8
replies
9.4k
views
Pre-processing of RNA-Seq data for WGCNA and DGE
WGCNA
RNA-Seq
DESeq2
voom
removebatcheffect()
updated 8.1 years ago by
Peter Langfelder
★ 3.0k • written 8.1 years ago by
gokce.ouz
▴ 70
4
votes
8
replies
23k
views
Variance stabilising transformation for machine learning
deseq2
vst
rna-seq
machine-learning
updated 5.2 years ago by
Michael Love
42k • written 5.2 years ago by
Manik Garg
▴ 20
1
vote
8
replies
2.1k
views
time series, block by patient, between and within comparisons limma-voom
rna-seq
time series
patient
limma-voom
updated 6.1 years ago by
Aaron Lun
★ 28k • written 6.1 years ago by
aec
▴ 90
0
votes
8
replies
2.5k
views
Isoforms detection using bioconductor
bioconductor
biomart
granges
rna-seq
updated 7.2 years ago by
Michael Lawrence
★ 11k • written 7.2 years ago by
seungjoon.kim
• 0
0
votes
8
replies
2.4k
views
DESeq2 Multi factor, multi time point comparison design formula
rna-seq
deseq2
deseq
R
updated 6.2 years ago by
Michael Love
42k • written 6.2 years ago by
arun.thirumaran
• 0
4
votes
8
replies
1.4k
views
problem in designing matrix and batch effect
edger
rna-seq
matrix
updated 4.8 years ago by
James W. MacDonald
67k • written 4.8 years ago by
alihakimzadeh73
• 0
3
votes
8
replies
3.8k
views
Normalisation in DESeq2
deseq2
rna-seq
updated 4.4 years ago by
Kevin Blighe
★ 4.0k • written 4.4 years ago by
thjnant
▴ 10
1
vote
8
replies
8.4k
views
DESeq2: Continuous and discreate variables in the design
deseq2
R
rna-seq
design
updated 3.8 years ago by
Michael Love
42k • written 4.9 years ago by
raquelgarza95
• 0
4
votes
8
replies
2.6k
views
Don't let TopGo to trimm long GO names
go
software error
topgo
rna-seq
updated 20 months ago by
James W. MacDonald
67k • written 4.4 years ago by
morteza.aslanzadeh
▴ 30
0
votes
7
replies
2.7k
views
Error reading kallisto files using tximport for cross species RNA-Seq analysis
kallisto
tximport
RNA-Seq
across species
updated 2.9 years ago by
nicolette.sipperly
• 0 • written 7.4 years ago by
urjaswita
▴ 40
2
votes
7
replies
2.3k
views
RNA-seq with Salmon and tximport
RNA-seq
Salmon
tximport
updated 5.1 years ago by
James W. MacDonald
67k • written 5.1 years ago by
woojoy14
• 0
8
votes
7
replies
5.8k
views
Further clarification on when not to use duplicateCorrelation with technical replicates (RNA-seq)
limma
duplicateCorrelation
rna-seq
technical replicates
biological replicates
updated 5.0 years ago by
Aaron Lun
★ 28k • written 5.0 years ago by
paul.alto
▴ 50
3
votes
7
replies
5.8k
views
What does this error mean? - issue implementing SVA
sva
rna-seq
updated 6.5 years ago by
Sandip Darji
▴ 30 • written 8.7 years ago by
kmuench
▴ 40
6
votes
7
replies
4.3k
views
Dealing with (blood) contamination in RNA-seq data
rnaseq
rna-seq
linear model
limma
updated 7.6 years ago by
Aaron Lun
★ 28k • written 7.6 years ago by
Thomas Sandmann
▴ 90
5
votes
7
replies
3.9k
views
Deseq2 design for one condition over 8 different time point
deseq2
rna-seq
design matrix
deseq
updated 6.0 years ago by
Michael Love
42k • written 6.0 years ago by
thindmarsmission
▴ 10
3
votes
7
replies
2.0k
views
DESeq2 design formula: how to account for twin data (MZ and DZ)?
DESeq2
twins
design
formula
RNA-seq
7.0 years ago
Simone
• 0
5
votes
7
replies
2.6k
views
limma - blocking or regressing out nuisance variable
limma
voom
RNA-seq
updated 5.5 years ago by
James W. MacDonald
67k • written 5.5 years ago by
biomiha
▴ 20
50 results • Page
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