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Showing :
RNA-SEQ
•
reset
40
votes
44
replies
8.1k
views
Are published RNA seq data analyses often wrong in calculating p-values and FDR?
FDR
p-value
DESeq
RNA-Seq
updated 5.1 years ago by
Wade Davis
▴ 60 • written 5.7 years ago by
yjiangnan
▴ 10
18
votes
11
replies
15k
views
when to apply quantile normalization with voom in limma/voom framework with RNA-seq data?
limma voom
limma
voom
rna-seq
quantile normalization
updated 7.0 years ago by
Gordon Smyth
47k • written 7.0 years ago by
lassancejm
▴ 50
18
votes
12
replies
2.8k
views
Low-count filtering with uneven sequencing depth between samples?
edgeR
deseq2
rna-seq
independent filtering
updated 7.6 years ago by
Michael Love
39k • written 7.6 years ago by
Ryan C. Thompson
7.8k
15
votes
12
replies
1.8k
views
ZINB-WaVE + DESeq2 misses low-expressed, single-condition genes that look like they should be markers
deseq2
ZINB-WaVE
single cell
RNA-seq
differential expression
updated 4.4 years ago by
Michael Love
39k • written 4.4 years ago by
rory.kirchner
▴ 60
14
votes
9
replies
2.8k
views
Is Limma's removeBatchEffect() and log2() commutative?
limma
rna-seq
removebatcheffect()
updated 5.0 years ago by
alakatos
▴ 120 • written 6.8 years ago by
Ekarl2
▴ 80
14
votes
9
replies
12k
views
How does DESeq2 handle zero counts in one condition?
deseq2
deseq
rna-seq
8.1 years ago
gavrielmatt
▴ 30
12
votes
2
replies
2.7k
views
How does edgeR compare to lme4?
rna-seq
edger
mixed model
random effects
lme4
updated 6.6 years ago by
Gordon Smyth
47k • written 6.6 years ago by
Ekarl2
▴ 80
11
votes
8
replies
7.1k
views
Pre-processing of RNA-Seq data for WGCNA and DGE
WGCNA
RNA-Seq
DESeq2
voom
removebatcheffect()
updated 6.4 years ago by
Peter Langfelder
★ 2.7k • written 6.4 years ago by
gokce.ouz
▴ 70
10
votes
4
replies
3.3k
views
Filtering lowly expressed genes in voom-limma analysis
voom
limma
rna-seq
differential gene expression
updated 6.5 years ago by
Gordon Smyth
47k • written 6.5 years ago by
rubi
▴ 110
10
votes
10
replies
4.1k
views
Pre-processing RNA-seq data (normalization and transformation) for GSVA
gsva
gene set variation analysis
normalization
transformation
rna-seq
4.8 years ago • updated 4.0 years ago
rached
▴ 50
10
votes
9
replies
1.1k
views
Is there are better way to improve the design via blocking or contrasts while using limma-voom for the mentioned datasets?
limma-voom
voom
limma
rna-seq
updated 5.2 years ago by
Aaron Lun
★ 28k • written 5.2 years ago by
vd4mmind
• 0
9
votes
14
replies
6.8k
views
too many genes with low read-counts as differential expressed in DESeq2
deseq2
rna-seq
6.5 years ago • updated 6.4 years ago
anand_mt
▴ 80
9
votes
1
reply
2.8k
views
limma Time Course Experiment with many time points
limma
RNA-Seq
updated 7.3 years ago by
Aaron Lun
★ 28k • written 7.3 years ago by
arfranco
▴ 130
9
votes
11
replies
4.9k
views
Using 'tximport' library for downstream DGE after quantifying with Kallisto
tximport
Kallisto
rna-seq
updated 5.9 years ago by
pablo_garcia05
▴ 20 • written 6.8 years ago by
cguzman.bioinformatics
▴ 10
8
votes
11
replies
6.3k
views
Multi factor design edgeR/Deseq2
deseq2
edgeR
rna-seq
updated 7.5 years ago by
Gordon Smyth
47k • written 7.6 years ago by
gthm
▴ 20
8
votes
2
replies
5.2k
views
interpreting LRT results DESeq2
deseq2
LRT
RNA-Seq
updated 7.8 years ago by
Michael Love
39k • written 7.8 years ago by
solgakar@bi.technion.ac.il
▴ 90
8
votes
3
replies
2.0k
views
Testing three-way interactions in DESeq2
deseq2
rna-seq
rnaseq
interactions
updated 5.3 years ago by
Michael Love
39k • written 5.3 years ago by
red_bricks
▴ 50
7
votes
6
replies
475
views
Odd DESeq2 results - high significance in genes with too many zeros
deseq2
rna-seq
updated 2.6 years ago by
Michael Love
39k • written 2.6 years ago by
mbk0asis
• 0
7
votes
7
replies
1.0k
views
Using GenomicDataCommons R package to identify cases with both DNA methylation and gene expression data
R
gdc
rna-seq
dna methylation
updated 4.3 years ago by
Sean Davis
21k • written 4.3 years ago by
RichardJActon
▴ 50
6
votes
5
replies
1.4k
views
Incorporating subject ID as random effect for repeated measures RNA-Seq analysis
limma
rna-seq
mixed model
repeated measures
updated 4.4 years ago by
Gordon Smyth
47k • written 4.4 years ago by
mikhael.manurung
▴ 270
6
votes
6
replies
602
views
Design to analyze experiment with multiple samples from the same biological entity
rna-seq
design
linear model
glm
updated 3.0 years ago by
Steve Lianoglou
13k • written 3.0 years ago by
tmms
• 0
6
votes
7
replies
2.6k
views
Dealing with (blood) contamination in RNA-seq data
rnaseq
rna-seq
linear model
limma
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
Thomas Sandmann
▴ 80
6
votes
5
replies
1.0k
views
How to know if I should use voomWithQualityWeights() or not?
limma
RNA-Seq
voom
voomWithQualityWeights
edgeR
updated 2.9 years ago by
Gordon Smyth
47k • written 2.9 years ago by
pedrodcb
• 0
5
votes
7
replies
1.4k
views
limma - blocking or regressing out nuisance variable
limma
voom
RNA-seq
updated 3.7 years ago by
James W. MacDonald
62k • written 3.7 years ago by
biomiha
▴ 20
5
votes
5
replies
1.2k
views
Extract specific contrast from paired multifactor design matrix
edger
deseq2
rna-seq
updated 7.5 years ago by
James W. MacDonald
62k • written 7.5 years ago by
Johannes Rainer
★ 2.0k
5
votes
11
replies
2.1k
views
DESeq2 logfoldchange and interaction in model
deseq2
R
logfoldchange
rna-seq
3.6 years ago • updated 3.5 years ago
pierre.marin
• 0
5
votes
2
replies
13k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
updated 12 months ago by
rickap
• 0 • written 4.0 years ago by
aishu.jp
▴ 30
5
votes
4
replies
1.4k
views
RNA-seq Normalisation - normalise all samples in experiment or only the ones used in the comparison?
rna-seq
normalization
edgeR
limma
updated 5.9 years ago by
Gordon Smyth
47k • written 5.9 years ago by
juls
• 0
5
votes
3
replies
2.4k
views
Understanding edgeR coefficients and design
edger
r edger de
rna-seq
updated 7.5 years ago by
Gordon Smyth
47k • written 7.5 years ago by
gthm
▴ 20
5
votes
5
replies
3.7k
views
Differential Expression Analysis between conditions of single cell RNA-Seq
deseq2
scRNA-Seq
RNA-Seq
differential expression analysis
updated 3.9 years ago by
Michael Love
39k • written 3.9 years ago by
hamza_karakurt
▴ 50
5
votes
3
replies
920
views
Combining counts from non-replicate samples in RNA-Seq analysis for a synergistic system
edger
normalization
counts
RNA-seq
updated 3.6 years ago by
Aaron Lun
★ 28k • written 3.6 years ago by
Tima Karginov
• 0
5
votes
6
replies
911
views
RUVseq to remove a library size artefact in a zebrafish brain RNA-seq dataset
RUVSeq
RUV
RNA-seq
updated 2.3 years ago by
James W. MacDonald
62k • written 2.4 years ago by
karissa.barthelson
▴ 20
5
votes
5
replies
713
views
First principal component variance stabilised RNA-seq data correlates with total read counts per sample / library concentration
RNA-seq
PCA
control
DESeq2
Quality
11 months ago • updated 8 months ago
9906201a
▴ 20
5
votes
1
reply
640
views
DE analysis when large portion of transcriptome is changed
rna-seq
edger
ebseq
differential expression
updated 4.6 years ago by
Aaron Lun
★ 28k • written 4.6 years ago by
marhabaie
• 0
5
votes
3
replies
1.6k
views
Correct contrast for testing multi-level interaction effect in limma using combined factors design matrix
limma contrast matrix
limma design matrix
limma
rna-seq
limma voom
updated 5.8 years ago by
Aaron Lun
★ 28k • written 5.8 years ago by
Jane Park
• 0
5
votes
7
replies
2.0k
views
problem for Using RNA-seq data-set in WGCNA
WGCNA
RNA-seq
updated 4.6 years ago by
Peter Langfelder
★ 2.7k • written 4.6 years ago by
modarzi
▴ 10
5
votes
9
replies
664
views
GSVA perfomed on RSEM Expected Count data
GSVA
RNA-Seq
RSEM
Expected_Count
6 months ago
Yang Shi
• 0
5
votes
6
replies
654
views
is our edgeR code doing what we think it does?
edger
differential expression
rna-seq
updated 3.4 years ago by
Yunshun Chen
▴ 780 • written 3.4 years ago by
acs1990
▴ 10
5
votes
7
replies
2.5k
views
Further clarification on when not to use duplicateCorrelation with technical replicates (RNA-seq)
limma
duplicateCorrelation
rna-seq
technical replicates
biological replicates
updated 3.3 years ago by
Aaron Lun
★ 28k • written 3.3 years ago by
paul.alto
▴ 50
5
votes
5
replies
955
views
edgeR no residual degrees of freedom for a specific row of numbers in dispCoxReidInterpolateTagwise
edger
rna-seq
updated 7.0 years ago by
Aaron Lun
★ 28k • written 7.0 years ago by
Koen Van den Berge
▴ 180
5
votes
7
replies
1.1k
views
Co-expression between host and pathogen genes
edger
rna-seq
updated 6.8 years ago by
Aaron Lun
★ 28k • written 6.8 years ago by
jhj89
▴ 10
5
votes
3
replies
2.3k
views
Error when calculating DE using the classical approach in edgeR
edgeR
differential expression
rna-seq
updated 6.4 years ago by
Gordon Smyth
47k • written 6.4 years ago by
lynski008
• 0
5
votes
12
replies
3.7k
views
DESeq2 time series contrasts between timepoints
rna-seq
deseq2
updated 15 months ago by
AZ
▴ 30 • written 5.3 years ago by
gtho123
▴ 40
5
votes
4
replies
1.1k
views
Testing for DE genes between samples of isolated cells, accounting for expression in whole tissue
limma
rnaseq
rna-seq
model-matrix
multiple factor design
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
smurray
▴ 10
5
votes
3
replies
815
views
DESeq2 with multiple treatments but no replicates
deseq2
rna-seq
updated 3.5 years ago by
Michael Love
39k • written 3.5 years ago by
jpc41
▴ 20
4
votes
4
replies
1.5k
views
Average read counts and batch-effect correction in edgeR
edgeR
batch-effect
RNA-seq
read counts
ngs
updated 4.0 years ago by
Aaron Lun
★ 28k • written 4.0 years ago by
Roman
▴ 20
4
votes
3
replies
18k
views
Fisher test in Rna seq
rna-seq
rnaseq
r
updated 3.5 years ago by
Gordon Smyth
47k • written 4.1 years ago by
g.k
• 0
4
votes
6
replies
2.6k
views
DESeq2 vst normalization: Do I have a heteroscedasticity problem and if so, what can I do about it?
deseq2
normalization
rna-seq
TCGA
vst
updated 3.9 years ago by
Michael Love
39k • written 3.9 years ago by
Marc Saric
▴ 70
4
votes
1
reply
2.2k
views
Using R package 'limma' on pre-normalized counts and the effect of non-equivalent reads/sample on limma linear model
limma
rna-seq
multi-mapped reads
normalization
microbiome
updated 6.3 years ago by
Steve Lianoglou
13k • written 6.3 years ago by
tealfurn
▴ 10
4
votes
11
replies
1.6k
views
Enquiry about the summarizeOverlaps() function
summarizeoverlaps
summarizedexperiment
count matrix
low counts
rna-seq
updated 7.0 years ago by
Valerie Obenchain
★ 6.7k • written 7.0 years ago by
shania90.lk
▴ 10
50 results • Page
1 of 1
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Replies
Comment: transcript name mapping between human and mouse
by
Eric
• 0
It works very well, and thanks for such a great answer! Because I use the old version gtf from GENECODE (vM12), the id version does not mat…
Comment: How to normalize long-read RNA-seq data for comparison with short-reads
by
Bernardo
• 0
Just did that, thank you for the suggestion.
Comment: Empty ChipQC Report
by
Okan
• 0
Hi, I have the same issue. Have you found any solution or any reason why this is happening?
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
Gordon Smyth
47k
Am I understanding correctly that you have 24 RNA-seq libraries in total, 6 from the pooled samples and 18 from regular samples with biolog…
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
k.roelfs
• 0
I'm sorry, I know it is kinda confusing... We have 6 pools in total: Phenotype A: T1a, T2, T1b, T1c Phenotype B: T1, T2 Each pool consist…
Votes
Answer: transcript name mapping between human and mouse
Comment: How to normalize long-read RNA-seq data for comparison with short-reads
Volcano Plot in R
Answer: Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
Answer: Replicating iDEP parameters in DESeq2; two conditions and an interaction term.
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