Bioconductor RNA-Seq

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deseq2 edgeR rna-seq

updated 4 hours ago by Elizabeth • 0 • written 8.8 years ago by gthm ▴ 30

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rna-seq edgeR

updated 3 days ago by Gordon Smyth 50k • written 10 days ago by Netanel • 0

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Comment: Differential peaks that were not identified in diffbind were identified in diseq by ATpoint ★ 4.0k

Make an MAplot. There is almost certainly an issue with normalization here given the very biased distribution of logFCs as in this Volcano.

Comment: significant difference of p-values with dream() after updating VariancePartition by gabriel.hoffman ▴ 100

See variancePartition updates for [v1.33.11](https://diseaseneurogenomics.github.io/variancePartition/news/index.html#variancepartition-133…

Comment: A ComBat-seq issue: the covariates are confounded by W. Evan Johnson ▴ 850

This is only partially confounded because there is overlap between the batches -- they both have GFP controls that can be used to remove ba…

Answer: Different number of exons in reference genome affects DEXSeq analysis by Alejandro Reyes ★ 1.9k

Likely the new annotation that you are using has more annotated isoforms, and thus the exons are splitted into more disjoint exonic bins. I…

Answer: Adding columns to DEXSeqResults object by Alejandro Reyes ★ 1.9k

I'd have a look into the match function, that could give you a mapping between the ids in the dxr1 object and your data frame of gene symbo…

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