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RNAseq123
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12
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4
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4.4k
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differential expression analysis of htseq data with edgeR/voom
voom
htseq
edgeR
limma
RNASeq123
updated 5.3 years ago by
Gordon Smyth
50k • written 6.9 years ago by
wd
▴ 30
1
vote
3
replies
4.3k
views
Using TMM normalized counts in Deseq2
RNAseq123
written 3.3 years ago by
mmicky5050
• 0
0
votes
3
replies
789
views
DESeq2 Statistic - Padj NA
DESeq2
RNAseq123
rnaseq
deseq2
metatranscriptomic
updated 16 months ago by
Michael Love
41k • written 16 months ago by
irish.prg
• 0
5
votes
6
replies
2.4k
views
Large difference in PCA plot based on LCPM (log counts per million) and VST (variance stabilizing transformations)
PCAtools
edgeR
pcaExplorer
RNASeq
RNAseq123
2.0 years ago
Alan
• 0
2
votes
5
replies
996
views
Can we massively improve RNA-Seq results by slightly reducing the counts in it?
edgeR
RNAseq123
updated 16 months ago by
Gordon Smyth
50k • written 17 months ago by
Istvan Albert
▴ 50
0
votes
1
reply
802
views
python and R
snpeff
python
tophat
r
RNAseq123
updated 2.7 years ago by
Kevin Blighe
★ 3.9k • written 2.7 years ago by
kusum
• 0
0
votes
1
reply
1.4k
views
Confused about understanding the output and statistics of BAM file after STAR aligning
RNAseq123
VariantFiltering
Alignment
VariantAnnotation
updated 17 months ago by
James W. MacDonald
65k • written 17 months ago by
Mohamed
▴ 30
2
votes
1
reply
995
views
Error in RNAseq123 analysis, unexpected symbol.
RNAseq123
Rna
updated 22 months ago by
Gordon Smyth
50k • written 22 months ago by
Mikołaj Mierzejewski
• 0
0
votes
2
replies
520
views
Deseq2 paird analysis in a cohort mixed of paired and unpaired samples
DESeq2
RNAseq123
14 months ago
fatemeh.peimani
• 0
1
vote
6
replies
3.1k
views
KEGG enrichment analysis for non model organism
RNAseq123
16 months ago
afsanarupa1
• 0
1
vote
1
reply
311
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 4 months ago by
ATpoint
★ 4.0k • written 4 months ago by
kaurprab
• 0
1
vote
1
reply
1.3k
views
featureCounts running too long
RNAseq123
updated 10 weeks ago by
difraiadomenico
• 0 • written 3.1 years ago by
demirkalecy
▴ 10
0
votes
0
replies
540
views
Plotting a line plot for genes across 4-species
RNAseq123
21 months ago
Mohamed
▴ 30
0
votes
0
replies
496
views
Circular RNA
CircularRNA
RNASeq
RNAseq123
3.2 years ago
rpokhre2
• 0
1
vote
1
reply
687
views
Confused about design for RNA seq experiment
RNAseq
ExperimentData
RNAseq123
RNA
ExperimentalDesign
updated 19 months ago by
ATpoint
★ 4.0k • written 19 months ago by
Mohamed
▴ 30
15 results • Page
1 of 1
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Replies
Comment: Which input file is used for DGEList in EgdeR?
by
i.atanasoai
• 0
Understood, Thank you very much for the reply, very kind.
Comment: Which input file is used for DGEList in EgdeR?
by
Michael Love
41k
"gene_counts_length_scaled.tsv file generated by the nf-core be exactly that?" No that is the counts scaled by the length. Counts + offset…
Comment: DESeq2 a lot of genes showing up as differentially expressed that only have 1 sa
by
Michael Love
41k
These genes are fine. They are just the ones where, by chance, the sample variance is below the sample mean. It happens. DESeq2 brings thes…
Comment: DEG with low counts
by
Michael Love
41k
These counts are too low, only a few samples above a count of 10. So for typical RNA-seq data that is underpowered to call differences. It…
Comment: DESeq filtering specific to contrasts
by
Michael Love
41k
If the counts are too low it in one group then you can't make the comparison either way (whether they are filtered out or just under-powere…
Votes
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
Comment: DEXSeq DEXSeqDataSet count matrix duplication
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