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Showing :
RSEM
•
reset
1
vote
7
replies
197
views
RSEM - tximport; where is effective_length information used? Implications for scRNA?
RSEM
tximport
scRNAseq
DESeq2
updated 1 day ago by
Michael Love
37k • written 9 days ago by
Kelen
• 0
0
votes
0
replies
195
views
Proper normalization of batch effected corrected raw rsem rnaseq values for downstream unsupervised analyses
limma
RNASeq
rsem
Normalization
7 months ago
svlachavas
▴ 800
7
votes
5
replies
9.7k
views
Expected counts from RSEM in DESeq2
RSEM
deseq2
expected_counts
raw_counts
updated 12 months ago by
Michael Love
37k • written 5.5 years ago by
chudar.chudar
▴ 10
1
vote
4
replies
568
views
TCGA data analysis
RSEM
15 months ago • updated 14 months ago
lily
▴ 20
0
votes
1
reply
343
views
tximport error importing expected counts file from RSEM
RSEM
tximport
updated 18 months ago by
Michael Love
37k • written 18 months ago by
Muyu
• 0
0
votes
2
replies
634
views
How well does "Trinity-Differential-Expression" work on normalized raw counts without RSEM abundance of estimation
Trinity
DifferentialExpression
RSEM
RNAseq
edgeR
19 months ago
harelarik
▴ 50
5
votes
6
replies
967
views
Transformation of raw RSEM gene expression estimates with variance stabilizing transformation and tximport R package
DESeq2
VST
rsem
tximport
19 months ago
svlachavas
▴ 800
0
votes
2
replies
607
views
Use of RSEM counts in WGCNA
rsemmed
RSEM
WGCNA
19 months ago
harelarik
▴ 50
1
vote
4
replies
474
views
tximport abundanceCol parameter not functioning for rsem data
tximport
rsem
transcript abundance
TPM
FPKM
updated 20 months ago by
Michael Love
37k • written 21 months ago by
m.metsger
• 0
6
votes
13
replies
1.7k
views
How to use RSEM value to find differentially expressed genes
RSEM
limma
edgeR
voom
normalization
updated 22 months ago by
Gordon Smyth
45k • written 22 months ago by
harelarik
▴ 50
0
votes
8
replies
645
views
Importing RSEM with tximport
tximport
RSEM
import error
updated 22 months ago by
Michael Love
37k • written 22 months ago by
harelarik
▴ 50
2
votes
2
replies
409
views
RSEM for DESeq2 without abundance matrix
tximport
deseq2
rsem
updated 23 months ago by
Michael Love
37k • written 23 months ago by
le2336
▴ 20
1
vote
3
replies
331
views
RSEM and Tximport
RSEM
tximport
deseq2
updated 2.0 years ago by
James W. MacDonald
60k • written 2.0 years ago by
pthom010
• 0
4
votes
7
replies
1.5k
views
Analyzing TCGA RSEM Data with DESeq2 downloaded from FireBrowse
deseq2
tximport
tcga
rsem
firebrowse
updated 2.1 years ago by
Kevin Blighe
3.6k • written 2.1 years ago by
J. Smith
• 0
0
votes
0
replies
341
views
Is the ballgownrsem function still supported?
ballgown
rsem
iranges
2.5 years ago
YH
• 0
0
votes
6
replies
1.5k
views
Large fold changes after deseq2 when one condition has two zeros and small number the other condition has two zeros and a large number
rnaseq
rsem
deseq2
3.8 years ago
loliveira
• 0
0
votes
0
replies
540
views
IRanges error while input rsem data into ballgown : .Call2("solve_user_SEW0", start, end, width, PACKAGE = "IRanges")
ballgown
rsem
iranges
4.0 years ago
kicheol.kim
• 0
1
vote
0
replies
1.4k
views
IRanges error while input rsem data into ballgown : .Call2("solve_user_SEW0", start, end, width, PACKAGE = "IRanges")
ballgown
rsem
iranges
4.0 years ago
kicheol.kim
• 0
0
votes
1
reply
413
views
Using tximport on RSEM abundance date
rnaseq
rsem
tximport
updated 4.0 years ago by
Michael Love
37k • written 4.0 years ago by
raeganlynn123
• 0
1
vote
4
replies
644
views
DE analysis of orthologs with varying sequence lengths using RSEM+tximport+DEseq2: normalization for sequence length?
deseq2
sequence length normalization
RSEM
tximport
differential expression
updated 4.0 years ago by
Michael Love
37k • written 4.0 years ago by
al-ash
▴ 50
0
votes
5
replies
703
views
countsFromAbundance for gene-level RSEM import in tximport
tximport
rsem
updated 4.0 years ago by
Michael Love
37k • written 4.0 years ago by
celi0001
• 0
0
votes
0
replies
495
views
RSEM output in txtimport
RSEM
txtimport
4.1 years ago
gat2010
• 0
0
votes
8
replies
1.5k
views
Differences in gene length passed to DESeq2 by tximport reulted in different gene rankings
deseq2
tximport
rsem
saimon
updated 4.1 years ago by
Michael Love
37k • written 4.1 years ago by
LD
• 0
1
vote
18
replies
2.3k
views
Using tximport on rsem files produces error while trying to read in files
R
tximport
rnaseq
tpm
rsem
4.3 years ago
stephan.emmrich
• 0
2
votes
2
replies
574
views
tximport failing at importing TPM instead of FPKM
tximport
rnaseq
rsem
updated 4.3 years ago by
rosabellascottgm
• 0 • written 4.3 years ago by
fresard.laure
• 0
3
votes
13
replies
1.2k
views
tximport's parameter for countsFromAbundance doesn't work
tximport
rsem
edger
4.4 years ago
tim.ivanov.92
• 0
0
votes
1
reply
724
views
Data object format for deseq2
deseq2
R
RSEM
updated 4.5 years ago by
Michael Love
37k • written 4.5 years ago by
tim.ivanov.92
• 0
1
vote
2
replies
915
views
RNA-seq read alignment best practices for low-input samples
limma
differential gene expression
rnaseq analysis
alignment
RSEM
updated 4.5 years ago by
Aaron Lun
★ 27k • written 4.5 years ago by
goldberg.jm
▴ 10
3
votes
4
replies
2.3k
views
Importing RSEM transcript-level data using tximport
tximport
rsem
updated 4.6 years ago by
Michael Love
37k • written 4.6 years ago by
Patrick Kimes
▴ 10
1
vote
11
replies
2.4k
views
Tximport with RSEM error
RSEM
updated 4.7 years ago by
k.vitting.seerup
▴ 100 • written 4.7 years ago by
hs.lansdell
▴ 10
2
votes
7
replies
1.1k
views
Obtaining transcript names in RSEM
tximport
rsem
updated 4.8 years ago by
Michael Love
37k • written 4.8 years ago by
deena
▴ 20
0
votes
2
replies
756
views
Using RSEM reads for DESeq2
deseq2
rsem
updated 4.8 years ago by
Michael Love
37k • written 4.8 years ago by
deeptiptl74
▴ 10
3
votes
1
reply
1.0k
views
Filtering step in Differential analysis with RSEM values
rsem
differential gene expression
edger
updated 4.9 years ago by
Gordon Smyth
45k • written 4.9 years ago by
Biologist
▴ 90
0
votes
1
reply
1.3k
views
confusion with tximport options and resulting matrix
tximport
rnaseq
rsem
limma
voom
updated 4.9 years ago by
Gordon Smyth
45k • written 4.9 years ago by
arubio
• 0
1
vote
3
replies
1.5k
views
Differential analysis with RSEM normalized counts using limma without voom
limma
differential gene expression
tcga
rsem
updated 5.0 years ago by
James W. MacDonald
60k • written 5.0 years ago by
Biologist
▴ 90
6
votes
5
replies
2.5k
views
Clustering differential expression results from voomWithQualityWeights
RNA
limma
rsem
edgeR
updated 5.2 years ago by
Gordon Smyth
45k • written 5.2 years ago by
hrishi27n
▴ 20
0
votes
0
replies
2.0k
views
How to normalize-transform rsem.genes.results of TCGA RNA-seq v2?
tcga
rnaseq
rsem
R
normalization
5.3 years ago
panagiotis.mokos
▴ 40
0
votes
1
reply
1.3k
views
RSEM output in DeSeq2
RSEM
deseq2
count matrix
updated 5.4 years ago by
Michael Love
37k • written 5.4 years ago by
kmeusemann
• 0
25
votes
37
replies
7.6k
views
Possible ways of performing differential gene expression and analysis of RNA-Seq data from normalized counts of RSEM software
rnaseq
RSEM
TCGA
edgeR
normalization
updated 5.5 years ago by
Gordon Smyth
45k • written 5.5 years ago by
svlachavas
▴ 800
5
votes
9
replies
4.7k
views
Voom: need at least two non-NA values to interpolate
limma voom
voom
rsem
fpkm
5.8 years ago
komal.rathi
▴ 100
0
votes
8
replies
1.4k
views
Using Scran with RSEM/DEseq
scran
normalization
deseq2
rsem
edger
5.9 years ago
a.guerra
• 0
6
votes
4
replies
3.1k
views
RSEM expected counts to limma-voom
limma
voom
rsem
updated 6.0 years ago by
Gordon Smyth
45k • written 6.0 years ago by
Jake
▴ 90
4
votes
7
replies
1.1k
views
assigning differential exons to trascripts
dexseq
rsem
differential exon usage
differential gene expression
updated 6.0 years ago by
James W. MacDonald
60k • written 6.0 years ago by
Assa Yeroslaviz
★ 1.5k
1
vote
3
replies
1.5k
views
DESeq2 size factors estimation after RSEM quantification
deseq2
tximport
rsem
estimatesizefactors
6.1 years ago
Hugo Varet
▴ 80
44 results • Page
1 of 1
Recent ...
Replies
Comment: Error: BiocParallel errors
by
lxl13260684135
• 0
I came across the same problem, have you solved it?
Answer: How to get DESeq2 results for a list of genes
by
ATpoint
★ 1.3k
Standard rules apply for filtering in R. If the genes are the rownames of the results object `res` then: res[my_ges,] or if the colu…
Answer: ComplexHeatmap: Change font size of annotation axis values
by
nalaspina
• 0
Someone on the original post was kind enough to provide the answer (in bold below): row_ha = rowAnnotation(foo2 = runif(10), bar2 = anno…
Answer: setting sizeFactors from scran :: caculateSumFactors.
by
ATpoint
★ 1.3k
You can conveniently convert your SingleCellExperiment to a DESeqDataSet, see https://bioconductor.org/packages/devel/bioc/vignettes/scran/…
Comment: Combine GRanges(List) to a single GRanges with unified breakpoints
by
Rodrigo
• 0
@malcolm-cook-6293, thank you for your response, I'm working through your code as we speak.
Votes
Answer: setting sizeFactors from scran :: caculateSumFactors.
Answer: ComplexHeatmap: Change font size of annotation axis values
Comment: package 'BiocInstaller' is not available (for R version 3.4.4)
A: svaseq: how many and which surrogate variables to pick
A: svaseq: how many and which surrogate variables to pick
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