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Rna
•
reset
3
votes
6
replies
336
views
Limma: log2 of TMM values for RNA-Seq Analysis and PlotMDS()?
Log2
edgeR
RNA
limma
Normalization
11 weeks ago • updated 10 weeks ago
Sara
• 0
0
votes
0
replies
98
views
Correct GFF3 translation for FeatureCounts
RNASeq
FeatureCounts
Rna
3 months ago
rridley3
• 0
0
votes
1
reply
172
views
Confused about design for RNA seq experiment
RNAseq
ExperimentData
RNAseq123
RNA
ExperimentalDesign
updated 4 months ago by
ATpoint
★ 2.1k • written 4 months ago by
Mohamed
• 0
0
votes
0
replies
247
views
Job:
Scientist/Senior Scientist – Bioinformatics / Computational Biology
MassSpectrometryData
RNA
DifferentialExpression
5 months ago
António Miguel de Jesus Domingues
▴ 490
0
votes
2
replies
291
views
Rsubread align --R session aborts
RNA
Alignment
Rsubread
6 months ago • updated 5 months ago
sreekalasn
• 0
2
votes
1
reply
298
views
Error in RNAseq123 analysis, unexpected symbol.
RNAseq123
Rna
updated 7 months ago by
Gordon Smyth
47k • written 7 months ago by
Mikołaj Mierzejewski
• 0
1
vote
2
replies
482
views
Is it necessary to standardize Combat in R?
RNA
Combat
sva
R
8 months ago
takemoto
• 0
1
vote
1
reply
316
views
Description of the headers obtained from the Eligos2 output
methylation
RNA
native
epitranscriptomics
updated 11 months ago by
tcalvo
▴ 80 • written 11 months ago by
megha.hs28
▴ 10
0
votes
1
reply
382
views
Ensembl dataset version
tximport
rna
rnaseqGene
biomaRt
updated 13 months ago by
ATpoint
★ 2.1k • written 13 months ago by
st3472
• 0
0
votes
0
replies
310
views
Cross-cultivar RNA-seq analysis
RNA
Normalization
21 months ago
nancy
• 0
0
votes
4
replies
690
views
Fitting a continuous variable with batch effect using Limma. Also a question about normalization.
limma
edgeR
rna
22 months ago
Nirad
• 0
0
votes
3
replies
1.1k
views
Pearson correlation between gene expression and phenotype
edgeR
RNASeq
updated 2.2 years ago by
Gordon Smyth
47k • written 2.2 years ago by
weichengz
• 0
4
votes
3
replies
950
views
How to resolve NAs when annotating a diff gene list with org.Hs.eg.db terms?
annotation
rna
updated 2.3 years ago by
James W. MacDonald
62k • written 2.3 years ago by
anthony.nash
▴ 20
2
votes
7
replies
645
views
Affycoretools package, HTA 2.0 annotation for non-coding RNA
affy
rna
hta2.0
r
updated 2.7 years ago by
James W. MacDonald
62k • written 2.7 years ago by
Marco_aurelio
• 0
1
vote
1
reply
384
views
How to interpret coefficients of time course modeled data with DESeq2?
deseq2
RNA
updated 2.8 years ago by
Michael Love
39k • written 2.8 years ago by
agsousa
• 0
0
votes
6
replies
578
views
Bizarre Dispersion Plots
deseq2
RNA
RNASeq
updated 2.9 years ago by
Michael Love
39k • written 2.9 years ago by
stephen.hartley
• 0
0
votes
0
replies
508
views
Job:
Post-doctoral Fellowship at BC Cancer Research Center, Vancouver BC
single cell
RNA
Data analysis
Bioinformatics
Job
3.6 years ago
jmaanaki
• 0
1
vote
1
reply
736
views
how to filter genes in significant module?
RNA
WGCNA
filter
3.6 years ago
modarzi
▴ 10
0
votes
2
replies
550
views
DESeq and checkFullRank(modelMatrix)
RNA
DESeq2
updated 3.8 years ago by
Michael Love
39k • written 3.8 years ago by
Merlin
▴ 10
2
votes
1
reply
458
views
DESeq2 statistic and design
deseq2
RNA
updated 3.8 years ago by
Michael Love
39k • written 3.8 years ago by
Merlin
▴ 10
5
votes
5
replies
824
views
Association analysis between gene expression and Phenotype
rna
singlecell
limma
updated 3.9 years ago by
Aaron Lun
★ 28k • written 3.9 years ago by
hrishi27n
▴ 20
2
votes
3
replies
703
views
Number and percent of mapped reads from RNA seq analysis.
rna-seq
rna
updated 3.9 years ago by
Steve Lianoglou
13k • written 3.9 years ago by
Anthony Nash
▴ 20
1
vote
0
replies
597
views
Job:
1 Computational Biologist and 1 Molecular Biologist
computational biology
molecular biology
RNA
r-loop
ncRNA
Job
4.3 years ago
l.brown
▴ 10
0
votes
0
replies
578
views
R code for finding wobble/redundant positions in an RNA string
r
code
rna
wobble
5.0 years ago
fawazfebin
▴ 50
0
votes
1
reply
657
views
Time series comparison with DESEQ2
RNA
rnaseq
deseq2
updated 5.5 years ago by
Michael Love
39k • written 5.5 years ago by
hrishi27n
▴ 20
0
votes
1
reply
1.1k
views
RNA sequencing analysis for time point experiment using DeSeq2
deseq2
RNA
rnaseq
updated 5.5 years ago by
Michael Love
39k • written 5.5 years ago by
hrishi27n
▴ 20
1
vote
1
reply
850
views
Outliers - to remove or to not remove that is the question
outliers
rna
microarray
updated 5.7 years ago by
chris86
▴ 400 • written 5.7 years ago by
bhgyu
▴ 30
5
votes
4
replies
928
views
Experiment design contrasts any time edgeR
DGE
rnaseq
RNA
edger
updated 5.7 years ago by
londonflavido
• 0 • written 5.7 years ago by
Pietro
▴ 40
3
votes
6
replies
1.4k
views
Regressing effect of treatment on RNA-seq expected count from rsem.
RNA
rna-seq
science
bioinformatics
updated 5.8 years ago by
James W. MacDonald
62k • written 5.8 years ago by
hrishi27n
▴ 20
6
votes
5
replies
2.8k
views
Clustering differential expression results from voomWithQualityWeights
RNA
limma
rsem
edgeR
updated 5.8 years ago by
Gordon Smyth
47k • written 5.8 years ago by
hrishi27n
▴ 20
0
votes
0
replies
1.0k
views
Finding candidate binding sites for RNA binding proteins in R
R
bioconductor
RNA
mRNA
RNA binding proteins
5.9 years ago
Walter F. Baumann
▴ 10
3
votes
1
reply
1.3k
views
Targeted RNASeq/CaptureSeq DE Analysis
EdgeR
deseq2
RNA
differential expression
updated 6.4 years ago by
Aaron Lun
★ 28k • written 6.4 years ago by
tyssowski
• 0
0
votes
4
replies
4.4k
views
RNAseq Adjusting for Covariates?
RNA
rnaseq
Covariate
adjustment
deseq
updated 6.9 years ago by
Michael Love
39k • written 6.9 years ago by
sjs0282
• 0
0
votes
2
replies
973
views
Normalizzation indipentent data from RNAAeq experiment
rna
normalization
8.3 years ago
aristotele_m
▴ 30
34 results • Page
1 of 1
Recent ...
Replies
Answer: struggling to create a heatmap
by
ATpoint
★ 2.1k
I am recuctant to read through that wall of code. Here is what would work. Note that prefiltering makes sense before, not after running `DE…
Answer: collapseReplicates error after tximeta
by
Michael Love
39k
The issue is that `collapseReplicates` just collapses `counts` and doesn't account for multiple assays which you need for e.g. Swish or oth…
Comment: Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it
by
ATpoint
★ 2.1k
That having said, I recommend to inspect the MA-plots (`plotMA()`), at best the ones produced right from `res <- results(dds); plotMA(res)`…
Answer: Which genes are outliers in DESeq2?
by
ATpoint
★ 2.1k
You can take over some of the lines from `results()` to find them easily: ```r suppressPackageStartupMessages(library(DESeq2)) set.s…
Answer: Which genes are outliers in DESeq2?
by
Michael Love
39k
Outliers will have high maxCooks values in `mcols(dds)` -- the threshold is `qf(.99, p, n - p)` where n is sample size and p is number of c…
Votes
Significant reporter activity with mpralm function
Answer: I cannot install annotables package in Rstudio (macOS)
Answer: log2 of normalized counts for further analyzes
Answer: Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
Awards
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to
Ryan
• 0
Popular Question
to
Guido Hooiveld
★ 3.5k
Scholar
to
James W. MacDonald
62k
Commentator
to
Michael Love
39k
Popular Question
to
twtoal
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