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StatisticalMethod
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2
votes
2
replies
551
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2
medianofratiostransformation
StatisticalMethod
Normalization
dispersion
3 months ago
Linton
• 0
1
vote
2
replies
420
views
How to perform longitudinal analysis using EdgeR
StatisticalMethod
FoldChange
edgeR
RNASeq
4 months ago • updated 3 months ago
Sabiha
▴ 20
2
votes
2
replies
384
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
5 months ago • updated 4 months ago
Manthos
• 0
4
votes
2
replies
571
views
DecideTest vs topTAGs in edgeR
RNAseq
StatisticalMethod
edgeR
updated 5 months ago by
Gordon Smyth
50k • written 5 months ago by
alvaroperona1
▴ 10
3
votes
2
replies
657
views
Does this RNAseq experiment require a repeated measures approach?
StatisticalMethod
RNASeqData
edgeR
DESeq2
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
CDSPARKS
▴ 10
2
votes
3
replies
1.7k
views
deseq2 normalization and statistical method
normalization
deseq2
StatisticalMethod
updated 12 months ago by
ATpoint
★ 4.0k • written 13 months ago by
SH
• 0
2
votes
4
replies
724
views
Model comparison in mean-dispersion trend in DESeq2
DESeq2
StatisticalMethod
14 months ago
Loïc
• 0
0
votes
2
replies
714
views
Using Multi-Dimensional Scaling (MDS) to produce a vector in order to account for patient bias when constructing DGE lists from RNA-seq datasets in R?
PrincipalComponent
StatisticalMethod
edgeR
MultidimensionalScaling
Normalization
14 months ago
rcreswell01
• 0
1
vote
3
replies
876
views
Limma-voom with inverse probability weights (similar to sample weights)
limma
limma-voom
StatisticalMethod
updated 14 months ago by
Gordon Smyth
50k • written 14 months ago by
jay
• 0
0
votes
1
reply
520
views
DESeq2 paired multifactor test
DESeq2
StatisticalMethod
updated 16 months ago by
Michael Love
41k • written 17 months ago by
ijvechetti
▴ 10
0
votes
0
replies
592
views
Job:
Academic position in Non-clinical Biostatistics
JobPosting
StatisticalMethod
17 months ago
Laurent Gatto
1.6k
1
vote
2
replies
939
views
Limma adj.p.value the same for many different p.values
limmaGUI
limma
adj.p.value
StatisticalMethod
18 months ago
walkerrh95
• 0
0
votes
0
replies
859
views
Job:
Postdoctoral research scientist in computational biology/statistical genetics/functional genomics at Columbia University, USA
Alzheimer's
StatisticalMethod
Genetics
molecularQTL
FunctionalGenomics
19 months ago
gaow
• 0
2
votes
3
replies
3.1k
views
Differential gene expression and (adjusted) p-values
edgeR
StatisticalMethod
updated 23 months ago by
ruez.richard
• 0 • written 2.2 years ago by
tmms
• 0
0
votes
0
replies
555
views
Should I adjust p value for multi-levels (groups) and multi-variables?
StatisticalMethod
2.1 years ago
dong
▴ 10
6
votes
4
replies
967
views
Limma prametrization
limm
MathematicalBiology
limmaGUI
StatisticalMethod
2.3 years ago • updated 2.2 years ago
Giovanni
• 0
2
votes
7
replies
1.4k
views
EdgeR, tumor normal pair, no replicatie but multiple patients, to focus on individual patients not combined
edgeR
StatisticalMethod
2.6 years ago
Field -Ye
• 0
0
votes
2
replies
1.3k
views
To retrieve the transcript from gff file with the longest isoform
FunctionalAnnotation
StatisticalMethod
2.8 years ago
Aneesha
• 0
0
votes
1
reply
725
views
DESeq2 Design matrix
StatisticalMethod
DESeq2
updated 2.9 years ago by
Michael Love
41k • written 2.9 years ago by
Saif
• 0
6
votes
6
replies
1.6k
views
limma:arrayWeights() and the "prior.n" parameter for biological replicates? (PROTEOMICS)
Proteomics
limma
StatisticalMethod
updated 3.1 years ago by
Gordon Smyth
50k • written 3.1 years ago by
Steve
▴ 10
1
vote
3
replies
1.1k
views
How to assign random effect factor in DESeq2 desing?
StatisticalMethod
DESeq2
phyloseq
updated 3.2 years ago by
Michael Love
41k • written 3.2 years ago by
zardbid
• 0
0
votes
0
replies
1.2k
views
Why is `scale-free topology` estimated as -sign(slope) * (R^2) in WGCNA?
Network
StatisticalMethod
WGCNA
ExpressionData
3.3 years ago
jol.espinoz
▴ 40
0
votes
2
replies
714
views
p adjusted value not available
StatisticalMethod
DESeq2
updated 3.4 years ago by
Kevin Blighe
★ 3.9k • written 3.4 years ago by
marie
• 0
23 results • Page
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Comment: How to make DGEList and design matrix by edgeR package
by
prity6459
• 0
Thank you for your suggestion. Yes, I have made DGEList and design matrix in my script. But I was not entirely confident while making thos…
Comment: Finding DEGs by using edgeR
by
prity6459
• 0
Hello Gordon, Thanks for your response. By normalization, I meant that I have already removed lowly expressed genes from my count matrix. …
Answer: FilterByExpr low counts with small sample size
by
Gordon Smyth
50k
It doesn't seem to me that there is necessarily any problem with the analysis. You that there are "too many low-reads" but actually there …
Answer: How to make DGEList and design matrix by edgeR package
by
Gordon Smyth
50k
Your question is curious because you showed code that made a DGEList and design matrix in your previous post just 15 hours ago. Please h…
Answer: Finding DEGs by using edgeR
by
Gordon Smyth
50k
I am not clear what you mean that you have already normalized your data. How exactly did you normalize the data? The best way to do an edg…
Votes
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
Comment: DEXSeq DEXSeqDataSet count matrix duplication
Comment: Error in champ.load(): The following specified files do not exist
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