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Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
Michael Love
41k
Then for the remaining genes that you don't think are DE, just plot like this: ``` dds_sub <- dds[,dds$condition %in% c("x","y")] dropleve…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
samer
• 0
yes i turned it off @mikelove
Answer: autonomics: example("read_somascan", package = "autonomics") fails, but not on B
by
Mike Smith
★ 6.2k
It looks like `autonomics` uses `BiocFileCache` to create a local copy of the data and save repeat downloading. Given the (relatively) rec…
Answer: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
Michael Love
41k
I notice you are using `betaPrior=TRUE`. We have set to default and recommended `betaPrior=FALSE` for many years (since version 1.16 in 201…
Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance
by
samer
• 0
Thank you for your reply, but it is always the same case. Showing a significant p-adj when there isn't a difference between the 2 condition…
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Answer: WGCNA documentation not available
Comment: Can the limma package be applied to Log2 RUV-normalized data?
Comment: Can the limma package be applied to Log2 RUV-normalized data?
Answer: Correct the batch effect in methylation analysis in R
Comment: Why is DMRcate not available for Bioconductor version 3.18?
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