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batcheffect
•
reset
1
vote
3
replies
40
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
1 hour ago • updated 6 minutes ago
Alexandre
• 0
0
votes
2
replies
152
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
5 days ago • updated 3 days ago
catv
• 0
0
votes
8
replies
374
views
DESeq and SVA for batch effect
DESeq2
BatchEffect
sva
7 weeks ago • updated 6 weeks ago
michelafrancesconi8
• 0
1
vote
3
replies
343
views
DESEQ2 Batch Effect
DESeq2
BatchEffect
RNASeq
updated 7 weeks ago by
swbarnes2
★ 1.3k • written 7 weeks ago by
sap275
▴ 10
0
votes
0
replies
142
views
Can I combine collinear variables into a single value
BatchEffect
limma
7 weeks ago
Harrsha
• 0
2
votes
1
reply
244
views
Limma Question
DESeq2
BatchEffect
limma
RNASeq
updated 8 weeks ago by
Michael Love
41k • written 8 weeks ago by
sap275
▴ 10
0
votes
1
reply
271
views
When should I correct for batch effects
BatchEffect
SingleCellExperiment
updated 3 months ago by
James W. MacDonald
65k • written 3 months ago by
NeuroDaniel
• 0
0
votes
5
replies
518
views
Can SVs identified from sva be used for removeBatchEffect() in DESeq2
sva
DESeq2
BatchEffect
limma
updated 3 months ago by
ATpoint
★ 4.0k • written 3 months ago by
Xiao
• 0
2
votes
4
replies
609
views
Correct the batch effect in methylation analysis in R
BatchEffect
limma
methylationArrayAnalysis
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
kyj2226
• 0
1
vote
4
replies
606
views
controling for batch effect while preserving variation within repeated measures that unevenly distributed across batches
miRNA
BatchEffect
RNASeq
limma
5 months ago
Wending
• 0
0
votes
4
replies
567
views
How to correct for known and surrogate variables in DESeq2?
DESeq2
BatchEffect
sva
limma
updated 8 days ago by
Michael Love
41k • written 6 months ago by
Crystal571
• 0
0
votes
1
reply
434
views
Batch effect adjust in model matrix
BatchEffect
edgeR
updated 7 months ago by
ATpoint
★ 4.0k • written 7 months ago by
Giuseppe
• 0
3
votes
5
replies
819
views
Getting batch corrected counts after RUVSeq
BatchEffect
RUVSeq
RNASeq
DESeq2
updated 7 months ago by
ATpoint
★ 4.0k • written 7 months ago by
Shashank
• 0
2
votes
5
replies
1.3k
views
removing the batch effect for vst counts and defining the batches in removeBatchEffect function
DESeq2
BatchEffect
limma
updated 8 months ago by
Gordon Smyth
50k • written 8 months ago by
Sara
• 0
2
votes
3
replies
1.0k
views
bulk RNA-seq analysis using R : batch effect : One question concerning Combat-seq and one question concerning DESeq2 design = batch + condition
BatchEffect
sva
DESeq2
RNASeqData
limma
updated 8 months ago by
James W. MacDonald
65k • written 8 months ago by
D-Bug
▴ 10
3
votes
4
replies
850
views
lfcShrink() and batch effects in DESeq2
BatchEffect
lfcshrink
RNA-seq
DESeq2
8 months ago
Eva
▴ 10
0
votes
2
replies
597
views
smallRNA-seq analysis batch correction in limma
BatchEffect
limma
9 months ago
Eleonora
• 0
1
vote
1
reply
443
views
Best way to handle batch effects from difference Mice?
RNASeqData
BatchEffect
edgeR
limma
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
Sara
• 0
2
votes
3
replies
1.1k
views
PCA for QC should include subset of top variable genes or full set of genes in RNAseq data?
BatchQC
RNASeqData
sva
BatchEffect
updated 10 months ago by
ATpoint
★ 4.0k • written 10 months ago by
amnahsiddiqa
▴ 10
0
votes
0
replies
381
views
How to go for Batch correction in RNA-seq expeiment conducted based on imbalanced Experimental design ?
RNASeqData
BatchEffect
sva
10 months ago
amnahsiddiqa
▴ 10
1
vote
1
reply
483
views
WGCNA Cluster batch effect
BatchEffect
TCGA
WGCNA
updated 10 months ago by
ATpoint
★ 4.0k • written 10 months ago by
khaserdene99
• 0
2
votes
5
replies
1.1k
views
DESeq2 remove batch effect
BatchEffect
RNASeqData
WGCNA
DESeq2
updated 8 days ago by
Ashley
• 0 • written 10 months ago by
zxc741xb
• 0
1
vote
2
replies
957
views
Batch effect between biological replicates
BatchEffect
DESeq2
11 months ago
gtechbio
▴ 10
0
votes
4
replies
881
views
Combine RNA-seq
BatchEffect
R
rnaseq
updated 12 months ago by
swbarnes2
★ 1.3k • written 12 months ago by
Young Tae
• 0
2
votes
4
replies
1.4k
views
How to get TPM / FPKM after batch correction with DESeq2?
DESeq2
BatchEffect
tpm
fpkm
12 months ago
vk
• 0
2
votes
2
replies
853
views
Error in checkFullRank(modelMatrix)
DESeq2
BatchEffect
DifferentialExpression
updated 12 months ago by
ATpoint
★ 4.0k • written 12 months ago by
AZ
▴ 30
2
votes
3
replies
872
views
Batch correction and Deseq2
DESeq2
WGCNA
Normalization
NormalyzerDE
BatchEffect
updated 13 months ago by
ATpoint
★ 4.0k • written 13 months ago by
suphamon.j
▴ 20
0
votes
1
reply
609
views
How to identify the batch informations from the sample ID?
TCGAbiolinks
BatchQC
BatchEffect
updated 8 months ago by
wrab425
• 0 • written 13 months ago by
snijesh
▴ 20
3
votes
2
replies
722
views
Batch question Bulk RNA-Seq
BatchEffect
RNASeq
13 months ago
chf
• 0
2
votes
1
reply
1.4k
views
DESeq2 design after combatseq batch correction
DESeq2
BatchEffect
updated 14 months ago by
ATpoint
★ 4.0k • written 14 months ago by
Tyroone
• 0
0
votes
1
reply
477
views
Analysis with Batch correction
DESeq2
BatchEffect
updated 14 months ago by
Michael Love
41k • written 14 months ago by
giulia.lopatriello
• 0
1
vote
1
reply
740
views
Batch effect: Is it correct to put ~0 preparing the model.matrix to remove batch effect?
edgeR
model.matrix
BatchEffect
updated 14 months ago by
James W. MacDonald
65k • written 14 months ago by
greta
• 0
5
votes
2
replies
756
views
Sva and batch effect
limma
BatchEffect
sva
updated 14 months ago by
Gordon Smyth
50k • written 14 months ago by
Lenny186
• 0
1
vote
1
reply
1.6k
views
batch effect correction before DESeq2 analysis
DESeq2
BatchEffect
updated 15 months ago by
ATpoint
★ 4.0k • written 15 months ago by
luca.s
▴ 50
0
votes
0
replies
716
views
Trying to use a NanoString expression set with batch effects to do GSVA
GSVA
Normalization
BatchEffect
GSVAdata
NanoString
15 months ago
Zhijie
• 0
1
vote
0
replies
651
views
Use scran's normalization by deconvolution for each individual sample or over all samples
scran
Normalization
scRNA
BatchEffect
16 months ago
r_grau
▴ 10
1
vote
2
replies
875
views
Design formula with batch effects DESeq2 "Not full rank matrix" error.
DESeq2
BatchEffect
RNASeq
updated 18 months ago by
swbarnes2
★ 1.3k • written 18 months ago by
Flavio
• 0
3
votes
10
replies
1.9k
views
Fold Change calculation when Batch Effect is present in RNA-seq data (DEseq2)
DESeq2
BatchEffect
fold
18 months ago
eve.olszanowski
• 0
1
vote
2
replies
786
views
Modelling batch effect in DESeq2 - how to interpret the results?
DESeq2
BatchEffect
18 months ago
annamariabugaj
▴ 10
0
votes
1
reply
950
views
Batch effects in DESeq2: determination and model building
BatchEffect
batch
RNASeqR
formula
DESeq2
updated 20 months ago by
Michael Love
41k • written 20 months ago by
estafana.t98
• 0
0
votes
1
reply
860
views
Building model matrix to correct for batch effect with biological and technical replicates: Problem with linear correlations
MassSpectrometryData
BatchEffect
r
limma
updated 21 months ago by
Gordon Smyth
50k • written 21 months ago by
mfaleevs
• 0
1
vote
1
reply
695
views
RNA-Seq normalization and batch effect removal
RNASeq
BatchEffect
DESeq2
updated 22 months ago by
Michael Love
41k • written 22 months ago by
Alexandre
• 0
6
votes
7
replies
1.8k
views
Batch effect with one sample being in two batches
BatchEffect
DESeq2
22 months ago
lmrogers34
▴ 10
2
votes
1
reply
1.3k
views
removeBatchEffect: Coefficients not estimable when using "batch matrix" for `covariates` argument.
limma
BatchEffect
updated 23 months ago by
Gordon Smyth
50k • written 23 months ago by
nhaus
▴ 30
0
votes
1
reply
911
views
Differential methilation analysis batch effect removal
BatchEffect
combat
sva
edgeR
RRBSdata
updated 2.0 years ago by
Gordon Smyth
50k • written 2.0 years ago by
Mageiros
• 0
1
vote
1
reply
1.1k
views
How to determine if limma can adjust for batch effect
BatchEffect
limma
sva
DifferentialExpression
updated 2.2 years ago by
ATpoint
★ 4.0k • written 2.2 years ago by
nhaus
▴ 30
0
votes
0
replies
500
views
Is it possible to do batch correction on Ht-Seq raw counts instead of using normalized counts? What's the difference?
Normalisedcounts
Rawcounts
Ht-SeqRNAData
BatchEffect
2.2 years ago
Ety
• 0
1
vote
7
replies
1.3k
views
Comparison DESeq results using different model
Batch
BatchEffect
DESeq2
updated 2.2 years ago by
James W. MacDonald
65k • written 2.2 years ago by
dequattro.concetta
▴ 10
0
votes
2
replies
1.0k
views
Using DESeq2 to analyse multi-variate design resulting in testing the wrong parameter
batch
RNASeq
BatchEffect
DESeq2
updated 2.3 years ago by
Steve Lianoglou
★ 13k • written 2.3 years ago by
Jonas
• 0
0
votes
2
replies
924
views
Design formula in DESeq2
model
BatchEffect
hidden
Design
DESeq2
2.5 years ago
m.glymenaki
• 0
50 results • Page
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Comment: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
Alexandre
• 0
Hello James, Thank you very much for your answer and taking the time to read my post. I understand your remark, but isn't the approach o…
Comment: PCA plot suggestions
by
JKim
• 0
cross post: https://www.biostars.org/p/9593486/
Answer: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
James W. MacDonald
65k
You cannot correct for technical variability due to batch if samples are nested in batch. The technical and biological differences are comp…
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
James W. MacDonald
65k
You will need to show some reproducible code for anybody to track this down. Please note that `DESeq2` has been used by thousands of people…
Comment: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
Alexandre
• 0
My code for the sessionInfo() . If the pictures are too big, I will downsize them. THank you again for reading my post ! ```r > sessionInf…
Votes
Answer: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
Answer: Use of negative binomial model for exponential decay rate estimation using DESeq
C: null model and DEXSeqDataSet object in DEXSEQ
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
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