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Comment: Are transformed values from rlog /vst log2 normalized counts?
by
Eva
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10
I understood exactly what you said, but I got confused with the documentation and the info that gives about rlog because it seems to be tha…
Answer: featureCounts fails to load annotation file
by
James W. MacDonald
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I imagine Wei Shi will be along later with actual help, but for now do note that you can use an SAF file instead of a GTF. It's easy enough…
Comment: Error for AnnotationForge makeOrgPackageFromNCBI function
by
James W. MacDonald
67k
That error indicates that you have a file called NCBI.sqlite in your working directory that is missing some tables. You should delete that …
Comment: Are transformed values from rlog /vst log2 normalized counts?
by
James W. MacDonald
67k
The `DESeq2` package is meant to analyze count data! Most packages that are intended to be used for RNA-Seq data are going to want the raw …
Comment: Are transformed values from rlog /vst log2 normalized counts?
by
Eva
▴
10
Many thanks for you detailed answer, I really appreciate it.
Could you give me an example (or more if possible) of analysis using count-b…
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