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reset
4
votes
14
replies
5.5k
views
Including technical and biological replicates in Limma block analysis?
limma
microarray
block
paired
linear model
9.4 years ago • updated 9.3 years ago
vas
▴ 40
6
votes
14
replies
3.0k
views
limma with continous covariate depending on gene, 2 block variables, pairing and technical replicates
limma
covariate
block
paired samples
technical replicates
updated 8.4 years ago by
Aaron Lun
★ 28k • written 8.4 years ago by
eleonoregravier
▴ 70
7
votes
7
replies
8.3k
views
batch effect : comBat or blocking in limma ?
batch effect
combat
limma
block
updated 8.6 years ago by
Ryan C. Thompson
★ 7.9k • written 8.6 years ago by
eleonoregravier
▴ 70
1
vote
6
replies
1.5k
views
DESEQ2 blocking design doesn't match expectation !
deseq2
block
pca
7.8 years ago
akp
• 0
3
votes
5
replies
1.8k
views
Limma contrast matrix question/concern
limma
block
design and contrast matrix
8.8 years ago
choofd
• 0
1
vote
4
replies
2.7k
views
Remove batch effect using individuals as random in the model in Limma
batch effect
Coeficents non estimable
block
random effects
limma design matrix
updated 6.4 years ago by
Aaron Lun
★ 28k • written 6.4 years ago by
mheydarpour
▴ 10
11
votes
4
replies
1.8k
views
LIMMA dupcor() function time issue.
limma
microarray
block
updated 6 months ago by
Gordon Smyth
50k • written 5.2 years ago by
tleona3
• 0
0
votes
3
replies
1.3k
views
About experimental design of RNA-seq
time course
block
batch effect
WGCNA
updated 7.1 years ago by
Michael Love
41k • written 7.1 years ago by
xie186
• 0
4
votes
3
replies
979
views
Diffbind for regressing multiple confounding variables (blocking factors)
diffbind
block
regression
deseq2
chipseq
updated 3.4 years ago by
Rory Stark
★ 5.1k • written 4.5 years ago by
melnuesch
▴ 10
4
votes
3
replies
2.0k
views
Blocked design and Surrogate Variables Analsysis(SVA)
limma
sva
block
updated 2.1 years ago by
stuartthafass
• 0 • written 9.5 years ago by
kodream
▴ 20
2
votes
2
replies
1.3k
views
limma roast/mroast with patient-matched pre-/post- treatment
limma
roast
mroast
block
updated 7.8 years ago by
Ryan C. Thompson
★ 7.9k • written 7.8 years ago by
barbara.shih
• 0
0
votes
1
reply
1.3k
views
Test for differential variability with missMethyl (DiffVar) using block design
missmethyl
diffvar
design matrix
limma
block
updated 5.9 years ago by
lhuang7
▴ 50 • written 6.8 years ago by
Simone
▴ 180
0
votes
1
reply
927
views
Limma: Correct for location and batch effect
limma
BatchEffect
duplicatecorrelation
block
batch
updated 3.4 years ago by
Kevin Blighe
★ 3.9k • written 3.4 years ago by
Katherine
• 0
13 results • Page
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Replies
Comment: FilterByExpr low counts with small sample size
by
Gordon Smyth
50k
As always, it always helps to see code. Are you running `voom` or `voomLmFit`? Did you run `normLibSizes()` in edgeR? Are you starting with…
Comment: Batch correction and repeated measures
by
Gordon Smyth
50k
In your code, it was clear that `expr.mat` was a matrix rather than a DGEList, so it has to be a count matrix. If you have a DGEList object…
Comment: Methylation Analysis experiment design using Limma
by
Gordon Smyth
50k
I don't analyse methylation arrays so cannot advise on specific limma settings for that type of data. Please follow the advice of the methy…
Comment: Light difference when using coef vs omit a group when compare 3 groups.
by
Chris
• 0
Hi Gordon, thank you so much for further guidance! I discussed this issue with doctor know about Alzheimer and they don't expect significan…
Comment: Single-Cell Data Simulation from Bulk RNA-seq
by
wichaidi
• 0
Hi - I am wondering what you decided to do. I am currently in a similar condition where I need to create a toy scRNAseq data from bulk.
Votes
Answer: How many of my genes from my gene list are in each KEGG pathway?
Comment: Light difference when using coef vs omit a group when compare 3 groups.
DESeq2 variance transformation error and plot dispersion Estimate
A: DESeq2 variance transformation error and plot dispersion Estimate
ensembl to hgnc symbols creates duplicates
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