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cellranger
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How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 3.2 years ago by
Aaron Lun
★ 27k • written 3.2 years ago by
xingxd16
▴ 20
0
votes
1
reply
145
views
Aberrant number of cells
CellBarcode
DropletUtils
emptyDrops()
Cellranger
scRNAseq
updated 13 days ago by
rohitsatyam102
▴ 20 • written 3 months ago by
gregoire.destreel
• 0
0
votes
0
replies
108
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Read Cellranger outputs in R, combine as SingleCellExperiment
ReadInR
Cellranger
GEX+Features+VDJ
3 months ago
gregoire.destreel
• 0
3 results • Page
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Comment: edgeR: the F-statistics changed after removing lowly expressed gene
by
Gordon Smyth
45k
There is nothing to be gained by doing analyses without filtering. We don't recommend it.
Comment: edgeR: the F-statistics changed after removing lowly expressed gene
by
Pei
• 0
Thank you Prof. Smyth! In my case, I found that number of significant genes tend to decrease after filtering when using glmQLFTest but num…
Answer: edgeR: the F-statistics changed after removing lowly expressed gene
by
Gordon Smyth
45k
glmQLFTest() function is designed to be used after expression filtering. You should apply expression filtering, regardless of whether it re…
Comment: EnrichGO not working with db created from annotationhub
by
Lucía
• 0
I have this, can this be used for GO in R? ``` 115709282 LOC115709282 phenylalanine ammonia-lyase 115699364 LOC115699364 …
Answer: EnrichGO not working with db created from annotationhub
by
Lucía
• 0
Hi, thanks so much for all your replies and running the code and confirming. I have not seen any GO term annotations for Cannabis so far…
Votes
Answer: edgeR: the F-statistics changed after removing lowly expressed gene
Answer: edgeR: the F-statistics changed after removing lowly expressed gene
A: DESeq2: thousands of NA for pvalues?
Answer: Difference between raw counts and featureCounts for DESeq2
A: Differential expression of RNA-seq data using limma and voom()
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Gordon Smyth
45k
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Matthew McCormack
▴ 180
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jared.andrews07
▴ 30
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vanbelj
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James W. MacDonald
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Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia,
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