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Comment: Differential expression of FPKM from RNA-seq data using limma and voom() by Essam • 0

Gordon I appreciate your useful response here. Since the original fastq files were created before the GEO era, we are unable to locate them…

Comment: How to reverse complement some elements of a DNAStringSet by Christine Jones • 0

Fantastic, thank you

Comment: When running contrasts, does it use normalized read counts? by Katherine • 0

Ok, so the comment I was given where they said DeSeq2 should be with normalized counts is incorrect. the ```normalized_counts``` variable …

Answer: How to reverse complement some elements of a DNAStringSet by James W. MacDonald 67k

You should always try the vectorized method first, rather than looping. ``` > myFasta[myFasta_rc] <- reverseComplement(myFasta[myFasta_…

Answer: When running contrasts, does it use normalized read counts? by James W. MacDonald 67k

A contrast is a comparison of two or more coefficients. When you run `nbiomWaldTest` you are fitting the GLM and estimating coefficients. T…

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Comment: featureCounts fails to load annotation file

Answer: How to reverse complement some elements of a DNAStringSet

Answer: KEGGgraph result difference

Answer: What should be the normalization protocol for RNA seq data for WGCNA?

What should be the normalization protocol for RNA seq data for WGCNA?

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