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dropletutils
•
reset
7
votes
12
replies
7.0k
views
How to identify real cells in 10X RNA-seq ?
single-cell
10X
Cell calling
DropletUtils
cellranger
updated 5.1 years ago by
Aaron Lun
★ 28k • written 5.1 years ago by
xingxd16
▴ 20
5
votes
1
reply
4.4k
views
Error with DropletUtils::EmptyDrops()
DropletUtils
EmptyDrops
simpleSingleCell
updated 20 months ago by
Stefano Mangiola
▴ 10 • written 4.7 years ago by
shbrief
▴ 20
7
votes
2
replies
2.3k
views
Recommendations for combining multiple 10x runs into one SingleCellExperiment
singlecellexperiment
dropletutils
simplesinglecell
updated 5.5 years ago by
Aaron Lun
★ 28k • written 5.5 years ago by
Peter Hickey
▴ 740
4
votes
5
replies
2.3k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
21 months ago • updated 20 months ago
Peter Hickey
▴ 740
4
votes
5
replies
2.0k
views
emptyDrops identified less non-empty cells with lower total UMI counts?
DropletUtils
scRNA-seq
emptyDrops
simplesinglecell
cell calling
updated 3.5 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
C T
▴ 140
1
vote
3
replies
1.8k
views
10X FeatureBarcode producing too many "cells"
SingleCellExperiment
DropletUtils
SingleCellData
updated 3.3 years ago by
Aaron Lun
★ 28k • written 3.3 years ago by
msn
▴ 10
0
votes
4
replies
1.8k
views
Error importing molecule_info.h5 file generated by Cell Ranger 3.0.2 into DropletUtils
DropletUtils
h5
updated 5.1 years ago by
Aaron Lun
★ 28k • written 5.1 years ago by
Matthew Thornton
▴ 350
1
vote
3
replies
1.6k
views
Troubles with barcodeRanks()
DropletUtils
SingleCellExperiement
DelayedArray
simpleSingleCell
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
Steve Pederson
▴ 170
0
votes
8
replies
1.6k
views
DropletUtils::read10xCounts error
read10xCounts
DropletUtils
15 months ago
fdumetz
• 0
0
votes
7
replies
1.5k
views
DropletUtils::swappedDrops more cells than using Read10x with filtered_feature_bc_matrix files
10x genomics
DropletUtils
single cell RNA seq
updated 4.1 years ago by
Aaron Lun
★ 28k • written 4.1 years ago by
gil.stelzer
• 0
1
vote
6
replies
1.4k
views
DropletUtils::swappedDrops segfault memory not mapped
DropletUtils
2.9 years ago
wesselyf
• 0
2
votes
4
replies
1.3k
views
Error when reading data with DropletUtils::read10xCounts
DropletUtils
updated 5.9 years ago by
Aaron Lun
★ 28k • written 5.9 years ago by
fabrost
• 0
1
vote
2
replies
1.2k
views
Segmentation Fault with development versions of R, Bioconductor, and DropletUtils when processing output of CellRanger 3.0.2
DropletUtils
Bioc-devel
5.1 years ago • updated 5.0 years ago
Matthew Thornton
▴ 350
2
votes
5
replies
1.2k
views
Recommendations for running DropletUtils::swappedDrops() on multiplexed samples from multiple libraries
dropletutils
swappeddrops
updated 5.0 years ago by
Aaron Lun
★ 28k • written 5.0 years ago by
rosano
• 0
0
votes
3
replies
1.2k
views
Running DropletUtils::swappedDrops() on multiplexed samples from different libraries
swappeddrops
dropletutils
updated 4.4 years ago by
Aaron Lun
★ 28k • written 4.5 years ago by
rosano
• 0
1
vote
5
replies
1.2k
views
about DropletUtils and scRNA-seq
dropseq
dropletutils
droplet
updated 5.3 years ago by
Aaron Lun
★ 28k • written 5.3 years ago by
Bogdan
▴ 670
2
votes
4
replies
1.2k
views
Does hashedDrops support 'imbalanced' combinations of HTOs?
DropletUtils
scRNAseq
updated 3.2 years ago by
Aaron Lun
★ 28k • written 3.2 years ago by
Peter Hickey
▴ 740
0
votes
2
replies
1.1k
views
DropletUtils barcodeRanks bug (?)
dropletutils
updated 5.4 years ago by
Aaron Lun
★ 28k • written 5.4 years ago by
jmanning
• 0
2
votes
2
replies
1.1k
views
What`s new features in DropletUtils version 1.3.10 comapred to version 1.2.2
DropletUtils
Bioconductor
singel cell
updated 5.1 years ago by
Aaron Lun
★ 28k • written 5.1 years ago by
xingxd16
▴ 20
1
vote
1
reply
1.0k
views
Number of non-empty drops returned by emptyDrops() depends on value of test.ambience
DropletUtils
emptyDrops
10x
updated 3.2 years ago by
Aaron Lun
★ 28k • written 3.3 years ago by
Peter Hickey
▴ 740
0
votes
1
reply
1.0k
views
Can DropletUtils Package be used to identify cells for 10X VDJ data ?
DropletUtils
10X 5` TCR
single cells
updated 5.1 years ago by
Aaron Lun
★ 28k • written 5.1 years ago by
xingxd16
▴ 20
0
votes
2
replies
1.0k
views
Scalability of emptyDrops() running times
dropletutils
emptydrops
updated 4.9 years ago by
Aaron Lun
★ 28k • written 4.9 years ago by
rosano
• 0
0
votes
4
replies
1.0k
views
DropletUtils' emptyDrops() fails with non-integer counts
DropletUtils
updated 4.7 years ago by
Aaron Lun
★ 28k • written 4.7 years ago by
jmanning
• 0
2
votes
1
reply
996
views
barcodeRanks unusual warning "In smooth.spline(x[new.keep], y[new.keep], df = df, ...)"
DropletUtils
updated 2.9 years ago by
Aaron Lun
★ 28k • written 2.9 years ago by
rohitsatyam102
▴ 20
1
vote
4
replies
973
views
Is there a limitation to the number of cells swappedDrops can handle?
swappedDrops
DropletUtils
5.0 years ago
joseph.joonsang
• 0
0
votes
1
reply
956
views
Aberrant number of cells
CellBarcode
DropletUtils
emptyDrops()
Cellranger
scRNAseq
updated 24 months ago by
rohitsatyam102
▴ 20 • written 2.2 years ago by
gregoire.destreel
• 0
3
votes
2
replies
947
views
Should we apply DropletUtils on control case separately or on a common sce object.
DropletUtils
3.0 years ago
rohitsatyam102
▴ 20
1
vote
2
replies
904
views
In which order should you detect empty droplets / remove barcode swapping using DropletUtils?
dropletutils
updated 5.0 years ago by
s1437643
▴ 20 • written 5.0 years ago by
jma1991
▴ 70
3
votes
8
replies
888
views
DropletUtils read10xcounts adds the 12 Cell Multiplexing Oligo tags as gene names
DropletUtils
droplet
written 5 months ago by
pcantalupo
▴ 10
0
votes
1
reply
859
views
Using controlAmbience with multiple samples and conditions
DropletUtils
updated 2.3 years ago by
Aaron Lun
★ 28k • written 2.3 years ago by
jma1991
▴ 70
0
votes
1
reply
823
views
emptyDrops result is full NAs
scran
dropletUtils
scater
scRNA-Seq
4.9 years ago
hamza_karakurt
▴ 50
0
votes
1
reply
770
views
DropletUtils::swappedDrops - Kallisto instead of CellRanger?
DropletUtils
updated 3.4 years ago by
Aaron Lun
★ 28k • written 3.4 years ago by
mhalache
• 0
3
votes
1
reply
664
views
read10xCounts for Cell Ranger 7 Output
DropletUtils
updated 5 months ago by
ATpoint
★ 4.0k • written 18 months ago by
Dario Strbenac
★ 1.5k
0
votes
3
replies
653
views
insufficient unique points for computing knee/inflection points
DropletUtils
SingleCellData
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
p.vlachou-efstathiou
• 0
1
vote
1
reply
641
views
Input format for `combinations` in `hashedDrops`
DropletUtils
updated 2.3 years ago by
Aaron Lun
★ 28k • written 2.3 years ago by
mikhael.manurung
▴ 270
35 results • Page
1 of 1
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Replies
Answer: DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
by
Michael Love
41k
> I got many p values that had "1.000000e+00" and padj "1" and stat of "0" when I added the Log threshold of LFC >1 and LFC < -1...is this …
Comment: Trying to use enrichGO
by
fernanda.backsouza
▴ 10
With all my love, thank you Guido, GOxploreR beeing a big ally for me. I don't know how to be grateful right now.
Comment: DESeq2 output used for PCA plot on R studio
by
swbarnes2
★ 1.3k
You didn't generate it with an experiment, you made it up: Your PCA doesn't look like a good RNASeq experiment, because it's not.
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
swbarnes2
★ 1.3k
Your contrast is comparing LGR5 to Homeostasis. The bottom 2 are fine, it's the top one that is wrong. Are you sure this step isn't misla…
Comment: get BM error
by
James W. MacDonald
65k
I believe you need NCBI Gene IDs for KEGG, in which case you may need to map. The three genes you have shown here don't map, and of those t…
Votes
Answer: Adding a group of samples reduces DEGs in other groups
Answer: Gene set analysis: GSVA, Z-Score, and fold-changes
Answer: FilterByExpr low counts with small sample size
Answer: get BM error
Answer: Use of negative binomial model for exponential decay rate estimation using DESeq
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