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Answer: Quantile Normalization after rma or not?
by
James W. MacDonald
59k
You already quantile normalized when you ran `oligo::rma`, so you should not normalize a second time.
Answer: Using featureCounts and downloading Rsubread
by
James W. MacDonald
59k
You need to tell `BiocManager;;install` that you want to install the `Rsubread` package.
```
BiocManager::install("Rsubread")
library(…
Comment: featureCounts: 0% successfully assigned fragments
by
KMS
•
0
Dear ATpoint
I am exactly looking for read/gene count from BAM/SAM file either through ht-seq or feature count
feature count giving me 0% s…
Answer: Get MANE transcripts from TxDb.Hsapiens.UCSC.hg38.knownGene
by
James W. MacDonald
59k
There is the ncbiRefSeqSelect table that has the MANE and RefSeq Select transcripts. It's not just the MANE though.
```
> z <- makeTxDb…
Comment: Handling of missing values in limma
by
AyHi
•
0
Thank you, Gordon, for your reply.
I used sum(is.na(lmfit)) after lmFit, but somewhat it indicated there was no NA observations even when…
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