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3
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6
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82
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LogFC values in edgeR - w/wo intercept
design.matrix
logFC
intercept
edgeR
model.matrix
7 hours ago • updated 3 hours ago
JK Kim
• 0
3
votes
3
replies
111
views
Question on RNA-Seq single sample data analysis in EdgeR
R
edgeR
limma
RNASeq
Normalization
updated 1 day ago by
Gordon Smyth
45k • written 2 days ago by
mohammedtoufiq91
▴ 10
0
votes
1
reply
60
views
after calcNormFactors with EdgeR, how to get TMM normalized matrix?
Normalization
edgeR
updated 6 days ago by
Gordon Smyth
45k • written 7 days ago by
kevin
• 0
3
votes
3
replies
119
views
edgeR: the F-statistics changed after removing lowly expressed gene
glmQLFTest
edgeR
updated 7 days ago by
Gordon Smyth
45k • written 8 days ago by
Pei
• 0
1
vote
1
reply
86
views
could edgeR be applied to gene counts by gene family to make group comparisons?
edgeR
updated 13 days ago by
Gordon Smyth
45k • written 13 days ago by
eliza_3176
• 0
2
votes
6
replies
175
views
Variance explained (coefficient of determination) in edgeR
edgeR
14 days ago • updated 8 days ago
Gemma
• 0
0
votes
1
reply
66
views
Locating nearest genes in RRBS analysis
hiAnnotator
bsseq
DifferentialMethylation
edgeR
RRBSdata
updated 14 days ago by
Gordon Smyth
45k • written 14 days ago by
Mageiros
• 0
2
votes
2
replies
118
views
edgeR: How to display/plot individual miRNAs found to be differentialy expressed?
miRNAData
miRNA
DifferentialExpression
edgeR
20 days ago • updated 19 days ago
Sarah
• 0
0
votes
2
replies
171
views
Variance explained (coefficient of determination) in glmFit / glmLRT
edgeR
21 days ago • updated 19 days ago
Gemma
• 0
0
votes
1
reply
102
views
How to directly decet the outlier samples (libraries) in EdgeR pipeline
edgeR
updated 22 days ago by
Gordon Smyth
45k • written 22 days ago by
Mohamed
• 0
0
votes
0
replies
59
views
diffcyt - topTable function returns one total significance estimate instead of one per comparison
limma
diffcyt
edgeR
25 days ago
stefanos.bamopoulos
• 0
1
vote
1
reply
95
views
Should I keep libsizes?
Normalization
edgeR
updated 26 days ago by
Gordon Smyth
45k • written 26 days ago by
Barista
• 0
2
votes
7
replies
232
views
edgeR: topTags weird behavior - outputting NAs
edgeR
27 days ago • updated 23 days ago
venura
• 0
0
votes
2
replies
108
views
edgeR: Analyzing multiple factors with batch effects
edgeR
29 days ago
venura
• 0
0
votes
11
replies
422
views
EdgeR function nearestTSS() fails with org.Ss.eg.db
Sus_scrofa
edgeR
updated 28 days ago by
Gordon Smyth
45k • written 4 weeks ago by
Jon
• 0
0
votes
4
replies
265
views
Extremely different results for both EdgeR and DESeq2 analysis
edgeR
DESeq2
updated 29 days ago by
Gordon Smyth
45k • written 4 weeks ago by
melatoninixo
• 0
2
votes
5
replies
260
views
edgeR: Combining time series analysis with mock and treated samples
edgeR
4 weeks ago
venura
• 0
0
votes
2
replies
110
views
edgeR
rnaseq
edgeR
updated 5 weeks ago by
Gordon Smyth
45k • written 5 weeks ago by
edusbioinfo
• 0
0
votes
11
replies
320
views
Differential methilation analysis - Design matrix not of full rank
DifferentialMethylation
RRBSdata
edgeR
updated 14 days ago by
Gordon Smyth
45k • written 5 weeks ago by
Mageiros
• 0
5
votes
6
replies
257
views
Large difference in PCA plot based on LCPM (log counts per million) and VST (variance stabilizing transformations)
PCAtools
edgeR
pcaExplorer
RNASeq
RNAseq123
5 weeks ago
Alan
• 0
0
votes
1
reply
95
views
Differential methilation analysis batch effect removal
BatchEffect
combat
sva
edgeR
RRBSdata
updated 5 weeks ago by
Gordon Smyth
45k • written 5 weeks ago by
Mageiros
• 0
2
votes
4
replies
190
views
I want to clarify difference between below two codes for analysis of DEGs. I got two different answers with these two methods and would like to know …
edgeR
6 weeks ago
devi
• 0
0
votes
1
reply
92
views
Tool for metagenomic functional analysis with 4-factor study design
Metagenomics
DESeq2
metagenomeSeq
edgeR
KEGG
updated 6 weeks ago by
Michael Love
37k • written 6 weeks ago by
Aditya
• 0
0
votes
2
replies
331
views
RNA-seq: problems with edgeR installation
BiocManager
edgeR
updated 6 weeks ago by
ATpoint
★ 1.2k • written 7 weeks ago by
alam
• 0
0
votes
0
replies
90
views
edgeR testing interactions between two factors while controlling for blocking factor
edgeR
ExpressionData
RNASeqData
7 weeks ago
Mafalda
• 0
0
votes
1
reply
170
views
Why does my volcano plot look weird?
edgeR
volcano_plot
updated 8 weeks ago by
Gordon Smyth
45k • written 8 weeks ago by
melatoninixo
• 0
2
votes
17
replies
638
views
How to reduce the impact of one varaible in Deseq2 or edgeR for multivariate value analysis?
MultipleComparison
edgeR
DESeq2
written 8 weeks ago by
yuet
• 0
0
votes
0
replies
98
views
Create a Gene Enrichment GO plot from goana output
goana
RNASeq
edgeR
RnaSeq
GO
8 weeks ago
Raito92
▴ 60
2
votes
3
replies
227
views
Classic or GLM approach in edgeR, Why do I got less DEG in GLM approach?
edgeR
DEGs
updated 8 weeks ago by
Gordon Smyth
45k • written 8 weeks ago by
RR
• 0
15
votes
18
replies
502
views
Is goana function for Gene Ontology enrichement analysis suitable for both edgeR and DESeq2 DEGs lists?
DESeq2
edgeR
goana
GO
updated 8 weeks ago by
Gordon Smyth
45k • written 9 weeks ago by
Raito92
▴ 60
2
votes
1
reply
134
views
How to determine which model (GLM and QLF or classic edgeR) to use for DGE analysis?
edgeR
updated 9 weeks ago by
Gordon Smyth
45k • written 9 weeks ago by
melatoninixo
• 0
6
votes
2
replies
167
views
The number of controls versus treatment in differential expression analysis
DESeq2
edgeR
limma
updated 9 weeks ago by
Gordon Smyth
45k • written 9 weeks ago by
AZ
▴ 30
0
votes
1
reply
135
views
Edge R Error in quantile.default(zresid, probs = prob) : missing values and NaN's not allowed if 'na.rm' is FALSE
diffSpliceDGE
RNASeq
DifferentialSplicingWorkflow
AlternativeSplicing
edgeR
updated 9 weeks ago by
Gordon Smyth
45k • written 9 weeks ago by
zhaoqiao1027
• 0
1
vote
2
replies
197
views
Normalize ChIP-seq samples with different signal-to-noise ratio
csaw
edger
tmm
chipseq
updated 10 weeks ago by
Rory Stark
★ 4.4k • written 10 weeks ago by
maria.soler
• 0
0
votes
2
replies
150
views
Gene Onotology: Can't get the goana function to work
edgeR
GO
11 weeks ago
Adelyn
• 0
2
votes
5
replies
271
views
DESeq2 with baseline correction for gene expression differences
DESeq2
edgeR
Transcriptomics
updated 11 weeks ago by
Michael Love
37k • written 12 weeks ago by
R1337
• 0
1
vote
1
reply
171
views
edgeR for SDE analysis
RNASeqRData
edgeR
RNASeqR
RNASeqData
updated 3 months ago by
Gordon Smyth
45k • written 3 months ago by
vikycairoli
• 0
0
votes
2
replies
224
views
Extend comparisons between two groups to more groups in DESEQ2
edgeR
DEGs
DESEQ2
RNASeqData
3 months ago
Raito92
▴ 60
0
votes
2
replies
205
views
filterbyexp() in edgeR
edgeR
updated 3 months ago by
Gordon Smyth
45k • written 3 months ago by
Simon.garinet
• 0
0
votes
6
replies
405
views
Performing simple pairwise comparisons in DESEQ2
edgeR
DEG
RNASeq
DESEQ2
updated 3 months ago by
Michael Love
37k • written 3 months ago by
Raito92
▴ 60
2
votes
3
replies
328
views
Differential gene expression and (adjusted) p-values
edgeR
StatisticalMethod
updated 22 days ago by
ruez.richard
• 0 • written 3 months ago by
tmms
• 0
0
votes
3
replies
413
views
using the goana function from edgeR
RNASeq
edgeR
GO
GOSemSim
updated 3 months ago by
Gordon Smyth
45k • written 4 months ago by
David Rengel
▴ 70
1
vote
4
replies
343
views
Differential Splicing using edgeR
diffSpliceDGE
edgeR
DifferentialSplicing
4 months ago • updated 3 months ago
Assa Yeroslaviz
★ 1.5k
3
votes
6
replies
410
views
How to use subjunc for differential splicing analysis?
subjunc
edgeR
4 months ago
Assa Yeroslaviz
★ 1.5k
2
votes
2
replies
206
views
catchKallisto results are not counts
DifferentialSplicingWorkflow
catchKallisto
edgeR
DifferentialSplicing
4 months ago
Assa Yeroslaviz
★ 1.5k
0
votes
1
reply
208
views
First time Statistical Analysis of RNA-seq
edgeR
RNA-Seq
log2
foldchange
q_value
updated 4 months ago by
Gordon Smyth
45k • written 4 months ago by
johndavidgriner74
• 0
0
votes
5
replies
375
views
ANOVA-like vs direct contrast
edgeR
4 months ago
fcamus
• 0
2
votes
2
replies
989
views
EdgeR installation issue
limma
edgeR
DESeq2
4 months ago
aonghus
• 0
0
votes
2
replies
234
views
Filtering low coverage RRBS counts results in few reads. Any alternatives?
limma
edgeR
updated 5 months ago by
franceschini.gianmarco
• 0 • written 5 months ago by
altintas.ali
• 0
3
votes
2
replies
340
views
Multi-level design RRBS analysis with voomLmFit
edgeR
voomLmFit
limma
duplicateCorrelation
voom
6 months ago
altintas.ali
• 0
50 results • Page
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Comment: Error in h(simpleError(msg, call)) : when calling makeOrgPackagefromNCBI()
by
Lan Anh
• 0
Hello, Sorry for the late answer, I called several packages after my error and I do not know how it worked after that. Thank you for the h…
Comment: LogFC values in edgeR - w/wo intercept
by
JK Kim
• 0
Thanks, Dr. Smyth!
Comment: LogFC values in edgeR - w/wo intercept
by
JK Kim
• 0
Oops. I just corrected the URL. Thank you Dr. Smyth.
Comment: Fail to run RupRadar
by
Qingwei
• 0
Dear Sergi, Yeah, it works. ```r dm <- analyzeDuprates(bamDuprm,gtf,stranded,paired,threads, GTF.featureType="cDNA_match", GTF.attrType="ID…
Comment: LogFC values in edgeR - w/wo intercept
by
Gordon Smyth
45k
The link you've entered for "A guide to creating design matrices for gene expression experiments" doesn't work. Could you please correct th…
Votes
Comment: LogFC values in edgeR - w/wo intercept
Answer: LogFC values in edgeR - w/wo intercept
Answer: LogFC values in edgeR - w/wo intercept
Answer: broken chain import in liftOver
Answer: toGRanges help!
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