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Comment: `emptyDrops()` calling 'too many' non-empty droplets by Peter Hickey ▴ 560
I've had some further and ongoing discussion with 10x tech support. I've copied the initial reply below and will add any useful updates. I…
Comment: `emptyDrops()` calling 'too many' non-empty droplets by Peter Hickey ▴ 560
Interestingly, `DropletUtils::emptyDropsCellRanger()` gave ~20,000 non-empty droplets for the problematic capture, `C120_batch13_1`, with o…
Comment: `emptyDrops()` calling 'too many' non-empty droplets by Peter Hickey ▴ 560
Thank you, Imran, for your response. I hope to look more closely at those 'real barcode' and 'false positive barcodes' at some point and ma…
Comment: Can I create my own, private ExperimentHub instance? by sandmann.t ▴ 70
That's awesome. Thanks a lot for the pointer to the BiocHubServer!
Comment: Conversion of UniProt to Ensembl by Marcel Ramos 570
Note that this can also be done via the `UniProt.ws` package: ```r library(UniProt.ws) up <- UniProt.ws() univals <- head(keys(up, "UniPro…
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Answer: `emptyDrops()` calling 'too many' non-empty droplets
Answer: `emptyDrops()` calling 'too many' non-empty droplets
Comment: Bioconductor teaching week
enhancedVolcano: Why are only a few of my select labels showing up?
A: Differential expression of RNA-seq data using limma and voom()
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