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8 results • Page 1 of 1

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Comment: FilterByExpr low counts with small sample size by Gordon Smyth 50k

As always, it helps to see code. Are you running `voom` or `voomLmFit`? Did you run `normLibSizes()` in edgeR prior to `voom`? Are you star…

Comment: Batch correction and repeated measures by Gordon Smyth 50k

In your code, it was clear that `expr.mat` was a matrix rather than a DGEList, so it has to be a count matrix. If you have a DGEList object…

Comment: Methylation Analysis experiment design using Limma by Gordon Smyth 50k

I don't analyse methylation arrays so cannot advise on specific limma settings for that type of data. Please follow the advice of the methy…

Comment: Light difference when using coef vs omit a group when compare 3 groups. by Chris • 0

Hi Gordon, thank you so much for further guidance! I discussed this issue with psychology doctor who treat Alzheimer patients and they don'…

Comment: Single-Cell Data Simulation from Bulk RNA-seq by wichaidi • 0

Hi - I am wondering what you decided to do. I am currently in a similar condition where I need to create a toy scRNAseq data from bulk.

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Answer: How many of my genes from my gene list are in each KEGG pathway?

Comment: Light difference when using coef vs omit a group when compare 3 groups.

DESeq2 variance transformation error and plot dispersion Estimate

A: DESeq2 variance transformation error and plot dispersion Estimate

ensembl to hgnc symbols creates duplicates

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