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Comment: DispEsts plot
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Sep
• 0
The sample size is 50 for each group, (50 pre and 50 post), samples are paired. No. I have not included the factors to account for unwanted…
Comment: KEGG enrichment in R and gene IDs
by
Guido Hooiveld
★ 3.6k
Do: `BiocManager::install(version = "3.16")`. The packages are (apparently) up-to-date within Bioconductor version 3.15...
Comment: KEGG enrichment in R and gene IDs
by
Laia
▴ 10
Wow thanks. > tools:::.BioC_version_associated_with_R_version() [1] '3.16' > packageVersion("clusterProfiler") [1] '4.4.4' > packageVe…
Comment: KEGG enrichment in R and gene IDs
by
Guido Hooiveld
★ 3.6k
No, having `NA` in your vector will work. I did notice that I retrieved only a single `NA` (8th position), but you got 4. My Bioconductor …
Comment: DispEsts plot
by
Michael Love
39k
What are the sample size for the two groups? Have you included factors to account for unwanted variance?
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Comment: RUVr batch correction doubling number of DEGs
Comment: RUVr batch correction doubling number of DEGs
Answer: RUVr batch correction doubling number of DEGs
A: LogFC: how do you determine the cutoff for differentially expressed genes?
Confused about design for RNA seq experiment
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