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Answer: Correct script for featurecounts in Rsubread by Gordon Smyth 47k
See - https://bioconductor.org/packages/release/workflows/vignettes/RnaSeqGeneEdgeRQL/inst/doc/edgeRQL.html#quantifying-read-counts-for-eac…
Comment: Time-course, comparison of two groups by boczniak767 ▴ 720
Thank you very much @gordon-smyth ! I'm very happy that my test for interaction is okay. So I'll try to extend my test experiment to three …
Answer: Handling limma usage after running multiple imputation on the covariates with mi by Gordon Smyth 47k
limma is not designed to be used with multiply imputed covariates, so I can't offer a solution that uses the multiple imputes. Averaging t…
Answer: Correct script for featurecounts in Rsubread by James W. MacDonald 62k
Here's the relevant section from `?featureCounts` ``` Value: A list with the following components: counts: a data matrix c…
Comment: Can I run DESeq2 with SMART-Seq data by Assa Yeroslaviz ★ 1.5k
thanks, the filter is the other way around, true 🙈
Votes
A: LogFC: how do you determine the cutoff for differentially expressed genes?
Confused about design for RNA seq experiment
A: gene dispersion, what does it mean?
Answer: gene dispersion, what does it mean?
Limma time series spline help
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Popular Question to Michael Love 39k
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Scholar to Gordon Smyth 47k
Popular Question to Robert Castelo ★ 3.0k
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