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Showing :
normalization
•
reset
2
votes
2
replies
331
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
6 days ago • updated 5 days ago
Henry
▴ 10
1
vote
6
replies
388
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2
Normalization
updated 14 days ago by
Michael Love
41k • written 14 days ago by
RL
• 0
0
votes
2
replies
134
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
14 days ago
Marianna
▴ 20
1
vote
4
replies
240
views
EDAseq to normalise for both gene-length and GC-content
norma
EDASeq
EDA
Normalization
28 days ago
samuel.channon
• 0
0
votes
0
replies
131
views
TCGAbiolinks STAD project 450k Methylation Beta Value data
MethylationArray
normalize450K
TCGAbiolinks
Normalization
MethylationArrayData
6 weeks ago • updated 29 days ago
Giovanni Calice
▴ 160
2
votes
1
reply
272
views
Normalization factors when there is a possible unidirectional expression
limma
Normalization
DifferentialExpression
updated 8 weeks ago by
ATpoint
★ 4.0k • written 9 weeks ago by
Shaimaa Gamal
• 0
2
votes
2
replies
355
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
10 weeks ago
jackafiore
• 0
2
votes
3
replies
327
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 10 weeks ago by
James W. MacDonald
65k • written 10 weeks ago by
Shaimaa Gamal
• 0
1
vote
3
replies
539
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 11 weeks ago by
georgii.vdovin
• 0
0
votes
1
reply
255
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 12 weeks ago by
ATpoint
★ 4.0k • written 12 weeks ago by
Ömür Koray
• 0
2
votes
4
replies
516
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
12 weeks ago
Sabiha
▴ 20
1
vote
1
reply
377
views
DESeq2 Normalization for QC Samples
DESeq2
Normalization
updated 3 months ago by
Michael Love
41k • written 3 months ago by
phmai1148
• 0
2
votes
2
replies
544
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2
medianofratiostransformation
StatisticalMethod
Normalization
dispersion
3 months ago
Linton
• 0
0
votes
0
replies
230
views
SomaticSignatures normalization for trinucleotide motifs in reference genome
Normalization
SomaticSignatures
5 months ago
vasily.dobrovolsky
• 0
0
votes
6
replies
789
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
5 months ago
erika.maldonado-rosado
• 0
0
votes
0
replies
255
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
6 months ago
Florian
• 0
1
vote
11
replies
962
views
DESeq2 gene length normalisation
Normalization
DESeq2
updated 6 months ago by
Michael Love
41k • written 6 months ago by
cm15245
• 0
1
vote
4
replies
2.4k
views
How to process Nimblegen microarray .pair files to get normalized expression matrix
Microarray
Preprocessing
NimbleGen
Normalization
written 17 months ago by
Sarah
• 0
1
vote
5
replies
993
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI
normalization
limma
voom
Normalization
updated 9 months ago by
Gordon Smyth
50k • written 9 months ago by
manuelsokolov
• 0
0
votes
3
replies
584
views
Normalization of different input materials
Normalization
DESeq2
updated 9 months ago by
ATpoint
★ 4.0k • written 9 months ago by
altuda
▴ 10
0
votes
2
replies
723
views
Can VST-transformed community composition data be expressed as relative abundance?
vst
relativeabundance
Normalization
DESeq2
10 months ago
dwas
• 0
5
votes
11
replies
2.9k
views
Visualization of negative log CPM values
Visualization
Normalization
ggplot2
edgeR
written 10 months ago by
Sabiha
▴ 20
0
votes
3
replies
688
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq
normalization
PCR_efficiency
bias
intrasample_comparison
updated 11 months ago by
James W. MacDonald
65k • written 11 months ago by
FedeXander
• 0
1
vote
6
replies
1.7k
views
can negative value microarray matrix data analysed by limma directly
limma
microarray
normalization
updated 8 months ago by
Gordon Smyth
50k • written 3.6 years ago by
linouhao
▴ 20
4
votes
8
replies
2.1k
views
Can we use rlog or vst data values in limma for differential expression analysis?
DESeq2
limma
Normalization
R
updated 10 months ago by
Gordon Smyth
50k • written 16 months ago by
Sabiha
▴ 20
0
votes
2
replies
2.2k
views
Incorporating spike-ins to rna-seq analysis
Spike-in
RNASeqR
DESeq2
Normalization
rna-seq
updated 7 months ago by
manuel.fernandez
• 0 • written 2.0 years ago by
cicely.day
• 0
4
votes
4
replies
2.0k
views
Unlog transformed data in DESeq2
RNASeq
DESeq2
vst
rlog
Normalization
updated 4 months ago by
Gordon Smyth
50k • written 2.3 years ago by
mohammedtoufiq91
▴ 10
6
votes
11
replies
3.9k
views
How to properly average normalized counts of biological replicates
DESeq2
Normalization
RNASeqR
updated 5 weeks ago by
Priya
• 0 • written 3.1 years ago by
Marina
▴ 40
2
votes
5
replies
11k
views
Conversion from counts to FPKM
counts
fpkm
fpkm()
normalization
deseq2
updated 6 months ago by
Hamza
• 0 • written 5.3 years ago by
chiara.facciotto
• 0
0
votes
1
reply
1.4k
views
EdgeR norm.factor input
Normalization
edgeR
Normalization
edgeR
updated 4 months ago by
Gordon Smyth
50k • written 10.2 years ago by
Guest User
★ 13k
0
votes
3
replies
2.5k
views
Background correction with neqc
Normalization
Cancer
Normalization
Cancer
updated 5 months ago by
Gordon Smyth
50k • written 12.4 years ago by
Guest User
★ 13k
31 results • Page
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Replies
Comment: FilterByExpr low counts with small sample size
by
Gordon Smyth
50k
As always, it helps to see code. Are you running `voom` or `voomLmFit`? Did you run `normLibSizes()` in edgeR prior to `voom`? Are you star…
Comment: Batch correction and repeated measures
by
Gordon Smyth
50k
In your code, it was clear that `expr.mat` was a matrix rather than a DGEList, so it has to be a count matrix. If you have a DGEList object…
Comment: Methylation Analysis experiment design using Limma
by
Gordon Smyth
50k
I don't analyse methylation arrays so cannot advise on specific limma settings for that type of data. Please follow the advice of the methy…
Comment: Light difference when using coef vs omit a group when compare 3 groups.
by
Chris
• 0
Hi Gordon, thank you so much for further guidance! I discussed this issue with doctor know about Alzheimer and they don't expect significan…
Comment: Single-Cell Data Simulation from Bulk RNA-seq
by
wichaidi
• 0
Hi - I am wondering what you decided to do. I am currently in a similar condition where I need to create a toy scRNAseq data from bulk.
Votes
Answer: How many of my genes from my gene list are in each KEGG pathway?
Comment: Light difference when using coef vs omit a group when compare 3 groups.
DESeq2 variance transformation error and plot dispersion Estimate
A: DESeq2 variance transformation error and plot dispersion Estimate
ensembl to hgnc symbols creates duplicates
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