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openprimer
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How to define settings for design primers with openPrimeR?
openPrimeR
3.2 years ago • updated 3.1 years ago
marongiu.luigi
• 0
0
votes
2
replies
713
views
How to save primers in local file in openPrimeR
openPrimeR
updated 13 months ago by
Santiago Edilberto
• 0 • written 3.1 years ago by
marongiu.luigi
• 0
2
votes
9
replies
1.8k
views
How to load fasta file with openPrimeR?
openPrimeR
written 3.2 years ago by
marongiu.luigi
• 0
0
votes
1
reply
625
views
openPrimer check_constraints cannot compute Tm
openPrimeR
updated 23 months ago by
matthias-doering
▴ 40 • written 23 months ago by
wnussbau
• 0
0
votes
1
reply
342
views
How to save primers in a local file with openPrimeR
openPrimeR
updated 13 months ago by
Basti
▴ 780 • written 13 months ago by
Santiago Edilberto
• 0
0
votes
0
replies
656
views
openPrimeR can't find external dependencies
openPrimeRui
openPrimeR
4.9 years ago
Vinicius Henrique da Silva
▴ 40
0
votes
4
replies
594
views
Load templates from openPrimeR
PCRmultiplex
openPrimeR
14 months ago
Santiago Edilberto
• 0
0
votes
1
reply
604
views
openPrimeR does not recognize external MELTING installation
openPrimeR
updated 2.7 years ago by
matthias-doering
▴ 40 • written 2.8 years ago by
benedikt.kirchner
• 0
0
votes
1
reply
573
views
How to increase the number of primer set output in openPrimeR
openPrimeR
updated 2.8 years ago by
matthias-doering
▴ 40 • written 3.1 years ago by
marongiu.luigi
• 0
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Comment: How to retrieve gene ontology GO class from gene list?
by
bandconductor
• 0
Thanks, this is potentially a much faster way than parsing BiomRt. However, it is only returning one GO term right now (biomaRt returns a f…
Comment: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
James W. MacDonald
65k
Both `RUVseq` and `sva` are meant to be used to remove technical variability. The problem is the identification of variability that is tech…
Comment: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
Alexandre
• 0
Hello James, Thank you very much for your answer and taking the time to read my post. I understand your remark, but isn't the approach o…
Comment: PCA plot suggestions
by
JKim
• 0
cross post: https://www.biostars.org/p/9593486/
Answer: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
by
James W. MacDonald
65k
You cannot correct for technical variability due to batch if samples are nested in batch. The technical and biological differences are comp…
Votes
Answer: Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
Answer: Use of negative binomial model for exponential decay rate estimation using DESeq
C: null model and DEXSeqDataSet object in DEXSEQ
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
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