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qPCR
•
reset
3
votes
0
replies
479
views
Creating qPCRset for nondetects anlaysis from QuantStudio data
nondetects
qPCR
qPCRData
2.9 years ago
jhackett
▴ 30
3
votes
4
replies
2.8k
views
Using limma for the analysis of qPCR data
NormqPCR
EasyqpcR
qPCR
HTqPCR
limma
updated 2.4 years ago by
Ming
▴ 380 • written 3.2 years ago by
enricoferrero
▴ 660
1
vote
2
replies
1.6k
views
qRT-PCR Sample Maximization software analysis and proper experimental design advice
qPCR
Sample Maximization Analysis
Experimental Design
qRT-PCR
updated 8.9 years ago by
Gordon Smyth
50k • written 8.9 years ago by
Marcelo Laia
▴ 450
1
vote
1
reply
1.7k
views
Are the logFC confidence intervals returned by topTable adjusted for multiple comparisons?
Microarray
qPCR
limma
updated 9.5 years ago by
Gordon Smyth
50k • written 9.6 years ago by
Calin-Jageman, Robert
▴ 30
1
vote
7
replies
1.6k
views
EDGE: Persistent lrt() and odp() command pi0est() error due to low p values
edge
qpcr
qvalue
7.1 years ago • updated 6.1 years ago
joe.d.moore.ii
• 0
1
vote
3
replies
2.4k
views
ddCt calculation using bioconductor package
ddct
qPCR
updated 2.8 years ago by
Pengcheng Yang
▴ 60 • written 9.0 years ago by
gv
▴ 40
1
vote
0
replies
1.0k
views
HTqPCR package data layout - how flexible is it?
qPCR
qPCR
9.8 years ago
Guest User
★ 13k
1
vote
4
replies
1.8k
views
ddCT Package for StepOnePlus qPCR output
qPCR
ddCt
geneexpression
updated 6.7 years ago by
Zhang, Jitao David
▴ 120 • written 6.7 years ago by
rbronste
▴ 60
1
vote
1
reply
1.2k
views
qRT-PCR - reading tab-delimited file (biol & tech replicates)
qPCR
ReadqPCR
qPCR
ReadqPCR
11.2 years ago
boczniak767
▴ 720
1
vote
0
replies
1.8k
views
DE analysis of PCR array [was: dataset dim for siggenes]
qPCR
Normalization
multtest
limma
siggenes
9.5 years ago • updated 7.2 years ago
Gordon Smyth
50k
0
votes
3
replies
1.4k
views
RNASeq, differential expression between group, and large variance within groups
RNASeq
qPCR
edgeR
DESeq
RNASeq
qPCR
edgeR
DESeq
updated 13.1 years ago by
Naomi Altman
★ 6.0k • written 13.1 years ago by
Gordon Smyth
50k
0
votes
0
replies
721
views
alternative CDF (Tarca, Adi)
qPCR
cdf
probe
affy
qPCR
cdf
probe
affy
17.9 years ago
raffaele calogero
▴ 500
0
votes
0
replies
911
views
4 replicates, of 3 tissues- one good hyb (2xreps), one bad hyb (2xreps). Best way forward?
qPCR
affy
affydata
vsn
limma
gcrma
matchprobes
affyPLM
affyio
BRAIN
qPCR
affy
affydata
17.6 years ago
k. brand
▴ 420
0
votes
2
replies
933
views
how to 'bootstrap' expression data?
qPCR
GO
qPCR
GO
updated 16.5 years ago by
Kasper Daniel Hansen
★ 6.5k • written 16.5 years ago by
Johnstone, Alice
▴ 410
0
votes
1
reply
1.2k
views
R: Degradation plots - Affy package
Transcription
qPCR
Genetics
probe
affy
Transcription
qPCR
Genetics
probe
affy
updated 17.6 years ago by
Alberto Goldoni
▴ 360 • written 17.6 years ago by
Graeme Smethurst
▴ 10
0
votes
4
replies
1.2k
views
HTqPCR questions
qPCR
HTqPCR
qPCR
HTqPCR
updated 13.2 years ago by
alessandro.guffanti@genomnia.com
▴ 330 • written 13.2 years ago by
Heidi Dvinge
★ 2.0k
0
votes
2
replies
1.5k
views
HTqPCR : readCtData problem?
miRNA
qPCR
HTqPCR
miRNA
qPCR
HTqPCR
13.4 years ago
Heidi Dvinge
★ 2.0k
0
votes
2
replies
1.9k
views
reading in data in ReadqPCR
qPCR
Annotation
HTqPCR
qPCR
Annotation
HTqPCR
11.1 years ago
Guest User
★ 13k
0
votes
0
replies
972
views
qPCR workshops in autumn 2008
qPCR
qPCR
15.5 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
3
replies
962
views
HTqPCR and limma and factorial design with no replicates
Microarray
qPCR
Classification
limma
HTqPCR
ASSIGN
Microarray
qPCR
Classification
limma
updated 14.2 years ago by
Naomi Altman
★ 6.0k • written 14.2 years ago by
Andreia Fonseca
▴ 810
0
votes
0
replies
884
views
Noch freie Workshop Plätze im Oktober 2008
qPCR
qPCR
15.5 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
877
views
Noch freie qPCR Workshop Plätze im Oktober 2008
qPCR
qPCR
15.5 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
1.2k
views
Talk & Poster Call - qPCR 2009 Event
SNP
qPCR
Normalization
Visualization
Regression
Cancer
probe
PROcess
microRNA
SNP
qPCR
15.4 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
1
reply
1.3k
views
Talk & Poster - Final Call - qPCR 2009 Event
SNP
qPCR
Normalization
Visualization
Regression
Cancer
probe
PROcess
microRNA
SNP
qPCR
15.3 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
1.4k
views
qPCR NEWS - December 2008
qPCR
graph
PROcess
microRNA
qPCR
graph
PROcess
microRNA
15.3 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
3
replies
1.1k
views
Manual creation of Affybatch object to study highthrouput qPCR experiments.
miRNA
qPCR
affy
miRNA
qPCR
affy
updated 12.4 years ago by
james perkins
▴ 300 • written 12.4 years ago by
Guest User
★ 13k
0
votes
0
replies
1.3k
views
qPCR NEWS - February 2009
qPCR
Normalization
PROcess
microRNA
qPCR
Normalization
PROcess
microRNA
15.1 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
953
views
New microRNA & qPCR workshop in April 2009
SNP
qPCR
Normalization
Regression
microRNA
SNP
qPCR
Normalization
Regression
microRNA
15.0 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
615
views
High Throughput RTqPCR data
qPCR
qPCR
14.7 years ago
james perkins
▴ 300
0
votes
0
replies
926
views
PLS help: is it sound to normalized highthrouput qPCR arrays using vsn?
qPCR
Normalization
vsn
qPCR
Normalization
vsn
11.9 years ago
Ali Mohammadian
▴ 290
0
votes
0
replies
1.5k
views
HTqPCR
qPCR
limma
ddCt
HTqPCR
qPCR
limma
ddCt
HTqPCR
11.5 years ago
Heidi Dvinge
★ 2.0k
0
votes
0
replies
1.1k
views
qPCR 2011 Symposium Talk and Poster CALL
qPCR
Genetics
microRNA
qPCR
Genetics
microRNA
13.3 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
1.5k
views
qPCR event preview - April 2010
miRNA
qPCR
Normalization
probe
microRNA
cycle
miRNA
qPCR
Normalization
probe
microRNA
13.9 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
1.1k
views
qPCR plates containing multiple samples in HTqPCR
qPCR
HTqPCR
qPCR
HTqPCR
14.1 years ago
Heidi Dvinge
★ 2.0k
0
votes
1
reply
1.1k
views
HTqPCR and limma and factorial design with no replicates
Microarray
qPCR
Classification
limma
HTqPCR
ASSIGN
Microarray
qPCR
Classification
limma
updated 14.2 years ago by
Naomi Altman
★ 6.0k • written 14.2 years ago by
Andreia Fonseca
▴ 810
0
votes
0
replies
1.2k
views
NEW deadline - Talk & Poster Call - qPCR 2009 Event
SNP
qPCR
Normalization
Visualization
Regression
Cancer
probe
microRNA
SNP
qPCR
Cancer
15.2 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
4
replies
1.1k
views
HTqPCR only works for 384 qPCR plates
qPCR
qPCR
updated 13.9 years ago by
Heidi Dvinge
★ 2.0k • written 13.9 years ago by
Andreia Fonseca
▴ 810
0
votes
9
replies
1.5k
views
htqPCR question
miRNA
qPCR
limma
miRNA
qPCR
limma
updated 13.9 years ago by
Andreia Fonseca
▴ 810 • written 13.9 years ago by
Heidi Dvinge
★ 2.0k
0
votes
12
replies
1.8k
views
htqPCR question
miRNA
qPCR
Normalization
limma
miRNA
qPCR
Normalization
limma
updated 13.9 years ago by
Heidi Dvinge
★ 2.0k • written 13.9 years ago by
Andreia Fonseca
▴ 810
0
votes
4
replies
1.4k
views
Reading in data with HTqPCR
qPCR
Cancer
cycle
HTqPCR
qPCR
Cancer
cycle
HTqPCR
13.8 years ago
Heidi Dvinge
★ 2.0k
0
votes
0
replies
1.2k
views
Experimental design for RNA-Seq
Sequencing
qPCR
Cancer
Sequencing
qPCR
Cancer
13.8 years ago
Jakob Hedegaard
▴ 170
0
votes
4
replies
2.3k
views
HTqPCR to analyze Fluidigm 96.96 Dynamic Array data
qPCR
HTqPCR
qPCR
HTqPCR
updated 13.8 years ago by
Heidi Dvinge
★ 2.0k • written 13.8 years ago by
V. Oostra
▴ 30
0
votes
2
replies
1.7k
views
Bacterial genomics
Sequencing
Microarray
qPCR
genomes
Sequencing
Microarray
qPCR
genomes
updated 13.6 years ago by
Hervé Pagès
16k • written 13.6 years ago by
Mathieu Parent
▴ 30
0
votes
2
replies
1.0k
views
Advise on setting up a non-specific filter for differential expression
qPCR
qPCR
13.6 years ago
Lucia Peixoto
▴ 330
0
votes
2
replies
1.2k
views
Advise on setting up a non-specific filter for differential expression
qPCR
qPCR
13.6 years ago
Tobias Straub
▴ 430
0
votes
1
reply
1.2k
views
gcrma vs rma
Microarray
qPCR
probe
ROC
limma
gcrma
Microarray
qPCR
probe
ROC
limma
gcrma
updated 13.6 years ago by
Zhijin Wu
▴ 260 • written 13.6 years ago by
Lucia Peixoto
▴ 330
0
votes
2
replies
1.2k
views
questions concerning quantile normalization in HTqPCR
miRNA
qPCR
miRNA
qPCR
updated 13.5 years ago by
Heidi Dvinge
★ 2.0k • written 13.6 years ago by
Andreia Fonseca
▴ 810
0
votes
1
reply
926
views
normalization usinf the delta Ct method
qPCR
Normalization
qPCR
Normalization
updated 13.6 years ago by
Wolfgang Huber
★ 13k • written 13.6 years ago by
Andreia Fonseca
▴ 810
0
votes
0
replies
1.1k
views
qPCR Event Calendar - autumn/winter 2010
qPCR
Normalization
Clustering
probe
microRNA
qPCR
Normalization
Clustering
probe
microRNA
13.5 years ago
qPCR Platform - the reference in real-t…
▴ 170
0
votes
0
replies
743
views
Any code for simulation of qPCR studies?
qPCR
qPCR
12.1 years ago
Ali Mohammadian
▴ 290
50 results • Page
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Answer: New genes that did not exist in dds object suddenly showed up after degPatterns
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May be documented by the software you are using? https://github.com/lpantano/DEGreport
Answer: Doing comparison of RNA-seq data using Deseq2
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Take a look at our workflow, this starts from a beginner's level. You could export from Excel using CSV or TSV, then read into R. htt…
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This support site is meant to help people with questions about Bioconductor packages (WGCNA is not a Bioconductor package). You might try o…
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Yes that is correct
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50k
It is the minimum group size rather than the total number of samples that is relevant. `min.prop` is more a biological parameter rather th…
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A: Error object and replacement value dimnames differ, when changing column names o
Comment: What benchmark should I use for setting the EdgeR filterByExpr min.count paramet
Comment: What benchmark should I use for setting the EdgeR filterByExpr min.count paramet
DESeq2 normalization vs VST vs rlog
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