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Comment: Using edgeR and a spike-in to calculate absolute abundance by Miguel • 0

I have the same issue. As far as I understand, many of the approximations to compute differential abundances include normalization steps wh…

Comment: shinyMethyl - saving the file by dorota.komar • 0

ok, it seems that I can embed shinyMethyl as an app in Rmarkdown and it should allow me to keep an interactive character. No idea how to do…

Comment: using featureCounts (Subread) to count only spliced reads per gene - behavior of by Gordon Smyth 48k

The splitOnly option is designed to strictly return split reads, i.e., reads that identify exon junctions rather than simply read-pairs ove…

Comment: blockwiseModule crashes in the end and doesn't generate modules by aletheabrown051 • 0

Re: [drift boss][1] Have you fixed this problem yet? [1]: https://driftboss.io

Comment: Deseq2 calculated log2foldchange not consistent with RPM in IP-small RNA-seq by Shihui • 0

It makes sense that DeSeq2 normalized FPMs are more accurate. Thank you for the information!

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Normalisation of data using DESeq2 with multi-groups

Batch correction and Deseq2

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