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First principal component variance stabilised RNA-seq data correlates with total read counts per sample / library concentration
RNA-seq
PCA
control
DESeq2
Quality
3.2 years ago • updated 3.0 years ago
9906201a
▴ 20
0
votes
0
replies
805
views
AffyExpress choose relative path folder
folder
quality
QualityControl
AffyExpress
4.4 years ago
bastien_chassagnol
• 0
0
votes
1
reply
1.1k
views
Array quality metrics for large sample size
microarray
quality
updated 6.3 years ago by
Wolfgang Huber
★ 13k • written 6.3 years ago by
RV
▴ 10
0
votes
3
replies
2.7k
views
what does "mean base quality" mean?
base quality
quality
mean base quality
updated 8.6 years ago by
Mike Smith
★ 6.6k • written 8.6 years ago by
christiangriffioen
• 0
4 results • Page
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Answer: DESeq2 Apeglm Coefficient Reordering / Log2FoldChange Comparison Change
by
James W. MacDonald
68k
Yes, you can simply flip the sign of the logFC to reverse the direction of the comparison.
Comment: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
The argument for annotation_col is meant to be a `data.frame`, not a vector. The hint in the error is that it has the wrong number of dimen…
Comment: Heatmap of a transformed deseq object with pheatmap
by
caroline.zanchi
• 0
I used this argument in the second paragraph of code forthe heatmap, this is wehre I got the message Error in `[.default`(annotation_col, c…
Answer: Heatmap of a transformed deseq object with pheatmap
by
James W. MacDonald
68k
Ah, yes. The non-ASCII character error. My favorite! Please note that `pheatmap` is not a Bioconductor package, so you should be asking …
Comment: Heatmap of a transformed deseq object with pheatmap
by
caroline.zanchi
• 0
It is in ! It just took longer than planned :) ... aaaand now that I added the heatmap in I realize lits of genes really do not look like t…
Votes
Comment: Heatmap of a transformed deseq object with pheatmap
DESeq2: Question about the alpha in summary() and results()
Comment: How to load scRNA-seq data from GEO
limma correction for tumor purity
limma correction for tumor purity
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