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r
•
reset
2
votes
27
replies
2.3k
views
CRISPRseek package: be aware that as of Oct 2015, CRISPRseek works best for for regions < 200kb
crisprseek
sgrna
crispr
cas9
R
6.9 years ago • updated 6.8 years ago
alexgraehl
▴ 20
3
votes
26
replies
3.0k
views
How to prepare targets.txt file in codelink.
r
codelink
updated 7.3 years ago by
Diego Diez
▴ 760 • written 7.3 years ago by
Agaz Hussain Wani
▴ 260
2
votes
23
replies
2.2k
views
Coping with a label/class imbalance in DESeq2
deseq2
rnaseq
r
regression
updated 5.9 years ago by
Michael Love
37k • written 5.9 years ago by
bkellman
• 0
5
votes
22
replies
4.3k
views
sqliteSendQuery error in AnnotationForge bioconductor package
bioconductor
annotationforge
annotationdbi
annotation
r
updated 6.1 years ago by
Axel Klenk
▴ 990 • written 7.5 years ago by
Stephen H
• 0
1
vote
22
replies
3.0k
views
problem with makeOrgPackageFromNCBI when making an annotation package
bioconductor
R
annotationforge
updated 5.6 years ago by
theobroma22
▴ 10 • written 6.0 years ago by
wssdandan2009
• 0
0
votes
19
replies
1.1k
views
Paired Difference Analysis
deseq2
R
differential gene expression
5.0 years ago • updated 4.9 years ago
nhua
• 0
1
vote
19
replies
15k
views
Import bamfiles for RNAsequencing analysis
R
rnaseq
bam file
updated 4.3 years ago by
Gordon Smyth
46k • written 4.3 years ago by
Merlin
▴ 10
1
vote
18
replies
2.4k
views
Using tximport on rsem files produces error while trying to read in files
R
tximport
rnaseq
tpm
rsem
4.4 years ago
stephan.emmrich
• 0
3
votes
18
replies
2.0k
views
Annotating codelink data file
r
codelink
annotation
annotationdbi
updated 7.3 years ago by
Diego Diez
▴ 760 • written 7.3 years ago by
Agaz Hussain Wani
▴ 260
4
votes
17
replies
1.5k
views
Question about decideTests function and adj.p.value in "limma" R Package
limma
r
differential gene expression
updated 5.1 years ago by
Gordon Smyth
46k • written 5.1 years ago by
Biologist
▴ 90
3
votes
16
replies
1.9k
views
How to add pseudocount to DESeq2 dds object
deseq2
r
updated 9 months ago by
Stanley
▴ 10 • written 23 months ago by
nabiyogesh
▴ 10
3
votes
16
replies
2.5k
views
Any way to proceed duplicate removal for list of GRanges object ?
r
grangeslist
duplicate
updated 5.6 years ago by
Martin Morgan
25k • written 5.6 years ago by
Jurat Shahidin
▴ 70
26
votes
16
replies
27k
views
Differential expression analysis starting from TPM data
R
TPM
updated 5.0 years ago by
Gordon Smyth
46k • written 5.0 years ago by
cahidora
▴ 60
0
votes
15
replies
1.3k
views
Is the STRINGdb server down?
STRINGdb
R
protein
updated 3.2 years ago by
zhanghongjie000
• 0 • written 3.4 years ago by
shahroze786
• 0
7
votes
15
replies
1.5k
views
Differentially expressed genes selection with FC and FDR cutoff
edgeR
rnaseq
r
bioconductor
updated 4.2 years ago by
Gordon Smyth
46k • written 4.2 years ago by
Beginner
▴ 50
5
votes
14
replies
3.5k
views
Connection Refused on port 9000 - RCytoscape
rcytoscape
r
network
graphBAM object
updated 6.7 years ago by
Dan Tenenbaum
★ 8.2k • written 6.7 years ago by
alptaciroglu
▴ 50
0
votes
14
replies
2.2k
views
Error in ReactomePA
r
reactomepa
updated 6.1 years ago by
Guangchuang Yu
★ 1.2k • written 6.1 years ago by
Ram
▴ 20
5
votes
14
replies
2.9k
views
Possible ways to select differential expressed genes using DeSeq2 or edgeR
R
deseq2
edger
differential gene expression
fold change
updated 3 months ago by
ATpoint
★ 1.4k • written 4.1 years ago by
Beginner
▴ 50
22
votes
14
replies
2.3k
views
biomaRt error unable to fix: need help troubleshooting
biomaRt
RNASeqData
R
updated 3 months ago by
Neri0304
• 0 • written 4 months ago by
Dina
▴ 50
14
votes
14
replies
23k
views
LogFC: how do you determine the cutoff for differentially expressed genes?
rnaseq
differential gene expression
logfc
limma
R
updated 7 weeks ago by
OE
• 0 • written 5.1 years ago by
cms72
▴ 20
0
votes
14
replies
1.4k
views
Path to file in windows
bioconductor
R
windows
diffbind
updated 5.3 years ago by
Gord Brown
▴ 640 • written 5.3 years ago by
jorrenkuster
• 0
4
votes
14
replies
7.7k
views
Extracting custom post-normalization counts from DESeq2
deseq2
r
ruvseq
normalization
rnaseq
7.1 years ago
nickp60
• 0
1
vote
13
replies
1.4k
views
Why Differential expression results differ with edgeR and limma ?
limma
edgeR
differential gene expression
r
5.0 years ago
Biologist
▴ 90
7
votes
13
replies
1.2k
views
How to extract phastcons score for all protein coding genes and lncRNAs using GenomicScores?
genomicscores
granges
phastCons100way.UCSC.hg38
r
rtracklayer
updated 2.4 years ago by
Robert Castelo
★ 2.9k • written 2.4 years ago by
Biologist
▴ 90
7
votes
13
replies
2.2k
views
Finding the coordinates from a BCV plot in edgeR
r
edgeR
estimatedispersions
coordinate
6.7 years ago
pertille
• 0
2
votes
13
replies
4.9k
views
Calculating rpkm from counts data?
r
featurecounts
rpkm
tpm
edger
updated 5.0 years ago by
kristoffer.vittingseerup
▴ 20 • written 5.0 years ago by
Biologist
▴ 90
2
votes
13
replies
1.8k
views
problems filtering antigenomic probes from HTA 2.0
pd.hta.2.0
R
6.0 years ago • updated 5.5 years ago
s.munster
▴ 40
7
votes
13
replies
1.3k
views
Test whether expression levels of two gene sets are significantly different
R
DESeq2
gene set testing
gene expression
5.8 years ago
Jon Bråte
▴ 190
10
votes
13
replies
3.3k
views
Trouble identifying differentially expressed genes on Affymetrix Human Gene 2.0.ST using R with oligo and limma package
R
limma
oligo
differential gene expression
affymetrix microarrays
updated 7.1 years ago by
tree-ski
• 0 • written 7.1 years ago by
dennis.oezcelik
▴ 10
1
vote
12
replies
1.5k
views
How can I integrate set of compressedList in multiple list into one without duplication?
r
iranges
findoverlaps
IntegerList
List
6.0 years ago • updated 5.8 years ago
jian_liangli
• 0
1
vote
12
replies
561
views
Taking much longer time for same script after R upgrade to 4.0
R
2.2 years ago
hsiowa2
▴ 20
4
votes
12
replies
4.1k
views
Filtering step for differential analysis in edgeR
r
edger
differential gene expression
filtering
bioconductor
updated 4.3 years ago by
Gordon Smyth
46k • written 4.3 years ago by
Biologist
▴ 90
0
votes
12
replies
1.2k
views
DESeq2 different versions
rnaseq
r
deseq2
updated 4.8 years ago by
Michael Love
37k • written 4.8 years ago by
minie
• 0
0
votes
11
replies
2.6k
views
Remove NaN from a list or replace NaN with a value.
R
meta-analysis
updated 12 months ago by
Tanya
• 0 • written 3.8 years ago by
morteza.hadizade
▴ 10
8
votes
11
replies
1000
views
Creating design matrix in LIMMA
limma
microarray
R
5.6 years ago
sgblackpearl
• 0
6
votes
11
replies
5.5k
views
How do I split GRange object (set of genomic ranges with 2 metadata column) by chromosome?
granges
split
r
updated 8 months ago by
jon.klonowski
▴ 40 • written 6.5 years ago by
Jurat Shahidin
▴ 70
0
votes
11
replies
602
views
Advice on designing an EdgeR object in complex experimental setup ?
rRDPData
r
3 months ago
Mohamed
• 0
5
votes
11
replies
1.9k
views
DESeq2 logfoldchange and interaction in model
deseq2
R
logfoldchange
rna-seq
3.2 years ago • updated 3.1 years ago
pierre.marin
• 0
5
votes
11
replies
833
views
"associated_gene" attribute from biomaRt returns too few values - SNP(rsID dataset)
r
biomart
SNP
annotation
HUGO
updated 2.0 years ago by
Mike Smith
★ 5.7k • written 2.0 years ago by
aroso491
• 0
2
votes
10
replies
1.4k
views
DiffBind consensus peak help
diffbind
r
chipseq
updated 4.9 years ago by
Rory Stark
★ 4.4k • written 5.0 years ago by
reubenmcgregor88
• 0
4
votes
10
replies
1.1k
views
what should I do with many zero counts from Salmon quantification
Salmon
DESeq2
normalization
r
updated 3.6 years ago by
Michael Love
37k • written 3.6 years ago by
lkianmehr
• 0
2
votes
10
replies
919
views
Am I on the right tracks with a 3 factor design in Limma?
r
limma
design matrix
biobase
updated 4.1 years ago by
Gordon Smyth
46k • written 4.1 years ago by
reubenmcgregor88
• 0
7
votes
10
replies
7.3k
views
R: error while reading .h5 files from R using rdhf5 package
rhdf5
r
hdf5
updated 5.1 years ago by
Wolfgang Huber
13k • written 5.2 years ago by
him4u324
• 0
5
votes
10
replies
1.1k
views
caught segfault error when loading S4Vectors package in R
R
S4Vectors
segfault
updated 3.5 years ago by
Martin Morgan
25k • written 3.5 years ago by
ecg1g15
• 0
4
votes
10
replies
7.5k
views
How can I extract sequences from a FASTA file for each of the intervals defined in a BED file using R?
R
bioconductor
granges
6.5 years ago • updated 6.1 years ago
pertille
• 0
2
votes
10
replies
18k
views
Heatmap with error message: `x' must be a numeric matrix
r
heatmap
numericmatrix
updated 6.4 years ago by
Assa Yeroslaviz
★ 1.5k • written 6.4 years ago by
Biologist
▴ 90
0
votes
10
replies
1.3k
views
tximport Error in attr(x, "names") <- as.character(value) : 'names' attribute [2] must be the same length as the vector [1]
software error
RNAseq
tximport
R
updated 3.0 years ago by
Michael Love
37k • written 3.0 years ago by
caranlove
• 0
0
votes
10
replies
1.1k
views
How to get feature ID's from the raw codelink data.
r
codelink
7.3 years ago
Agaz Hussain Wani
▴ 260
6
votes
10
replies
3.9k
views
defining a function for multiple paired t.test in many rows of a microarray dataset
microarray
r
t.test
bioconductor
updated 7.6 years ago by
Gordon Smyth
46k • written 7.6 years ago by
svlachavas
▴ 800
2
votes
10
replies
6.3k
views
adjusted p-values and p-values problems!
pvalue
adjusted pvalue
r
bonferroni
rna-seq
updated 5.9 years ago by
Michael Love
37k • written 5.9 years ago by
fromhj304
▴ 10
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Comment: How to retrieve Entrez IDs using Gene Symbols?
by
Basti
▴ 390
Cross-posted : https://www.biostars.org/p/9535046/#9535054
Comment: An ESR essay about software design, and how it applies to Bioconductor
by
Gary
• 0
It's relevant for me, thank you for the information!
Comment: An ESR essay about software design, and how it applies to Bioconductor
by
Gary
• 0
Thank you for sharing!
Comment: conditional command within GRanges
by
Basti
▴ 390
I can't reproduce your issue because you did not provided an example of your dataset (example with `dput(head(data1))`, but in the first ca…
Comment: Error in Create switchAnalyzeRlist
by
k.vitting.seerup
▴ 120
That sounds like you have the wrong version of the annotation compared to which version you used for the quantification?
Votes
Answer: WHat is the difference between DEG and TopVarGenes in DESEq2?
Answer: WHat is the difference between DEG and TopVarGenes in DESEq2?
A: EGSEA error during report generation: "only 0's may be mixed with negative subsc
Answer: mapToAlignments_issue
Analysis of peaks generated from Homer using Diffbind
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