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scRNASeq
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0
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120
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Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 14 days ago by
ATpoint
★ 4.0k • written 16 days ago by
Merav
• 0
0
votes
2
replies
195
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
5 weeks ago
rocanja
▴ 60
0
votes
1
reply
239
views
How to select cells that represents more accurately a cluster in a scRNA-seq dataset?
scRNAseq
updated 8 weeks ago by
ATpoint
★ 4.0k • written 8 weeks ago by
sodiogoes
• 0
1
vote
2
replies
773
views
ScRNAseq analysis scran :: quickcluster Error
scRNAseq
quickcluster
scRNA
scran
4 months ago
ruba-mahmoud
• 0
0
votes
2
replies
483
views
scRNA analysis : DEG between low and high-expressing cells
scRNAseq
6 months ago
Momo
• 0
0
votes
1
reply
378
views
FALSE error when checking a pre made sample data
Rstudio
scRNAseq
DESeq2
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
DrFizz
• 0
1
vote
5
replies
835
views
Recommendation for large.n in filterByExpr
scRNAseq
edgeR
SingleCellExperiment
8 months ago
duez.tolga
• 0
0
votes
1
reply
983
views
DESeq2 error after installing BiocManager
scRNAseq
RStudio
DESeq2
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
DrFizz
• 0
4
votes
3
replies
1.0k
views
Custom design for scRNA DGE with pseudobulking
scRNAseq
scrna
paireddesign
edgeR
pseudobulking
updated 8 months ago by
Gordon Smyth
50k • written 8 months ago by
garcia
• 0
0
votes
1
reply
408
views
If highly-expressed genes are upregulated, what happens to genes with lower expression after normalization?
scRNA
scRNAseq
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
Omer
• 0
0
votes
1
reply
549
views
library(topGO) query
topGO
scRNAseq
updated 9 months ago by
Basti
▴ 780 • written 9 months ago by
Francesco
• 0
0
votes
0
replies
343
views
adding noise to scRNA Seq counts
scRNAseq
9 months ago
Jiyuan
• 0
1
vote
11
replies
3.4k
views
Error: Bioconductor version cannot be validated; no internet connection?
scRNAseq
updated 5 months ago by
Manuel
• 0 • written 9 months ago by
Elham
• 0
2
votes
3
replies
1.3k
views
P-value histogram of testLinearModel is not uniform
smart-seq2
scRNAseq
scran
10 months ago
Lluís Revilla Sancho
▴ 730
0
votes
0
replies
466
views
News:
course - single-cell RNAseq data analysis with R and Bioconductor
Workshop
scRNAseq
11 months ago
info
▴ 10
0
votes
2
replies
667
views
Could not find function "Standard Chromosomes"
scrna
scRNAseq
updated 6 months ago by
Gabriella
• 0 • written 13 months ago by
sarah.jobbins
• 0
0
votes
2
replies
884
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell
RNASeq
RNASeqData
scRNAseq
updated 7 days ago by
wichaidi
• 0 • written 17 months ago by
irvin.ng
• 0
17 results • Page
1 of 1
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Replies
Comment: Opposite sign of LFC in count plots of DEGs (DESeq2)
by
winwater0928
• 0
Thank you for your comment! 1)"Your contrast is comparing LGR5 to Homeostasis." means (Lgr5)/(Homeostasis). Is it right? Then, I think if…
Answer: Reproducibility issue with scran together with lapply and BiocParallel
by
Aaron Lun
★ 28k
IIRC **BiocParallel** switches to a different RNG when inside its own functions like `bplapply` - specifically L'Ecuyer-CMRG, as opposed to…
Comment: DESeq2 output used for PCA plot on R studio
by
swbarnes2
★ 1.3k
This looks terrible for RNASeq data, because it's not RNASeq data. It's a matrix of pretty random numbers, which you know because you poste…
Comment: DESeq2 output used for PCA plot on R studio
by
Aaliya
• 0
I had taken this code from internet, but after reading about it and I did on my own, I would be making a post to ask for the suggestions if…
Comment: featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_ge
by
shuwangxinze1996
• 0
Hello, I used 48 cpus and 320G of memory to run this program. My bam files size are 7-9G, wonder how much CPU and memory is enough? Thank …
Votes
A: Are published RNA seq data analyses often wrong in calculating p-values and FDR?
A: How to explain how DESeq2 works to someone with zero bioinformatics background?
Answer: Interpret plot from DiffBind
Answer: How to retrieve gene ontology GO class from gene list?
Answer: How to retrieve gene ontology GO class from gene list?
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