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scRNASeq
•
reset
0
votes
1
reply
106
views
Can you find the correct transcript in scRNA seq data
scRNAseq
RNAseq
updated 9 days ago by
ATpoint
★ 4.0k • written 11 days ago by
Merav
• 0
0
votes
2
replies
190
views
Non-empty droplets versus good quality cells
CellRanger
emptyDrops
scRNAseq
10X
4 weeks ago
rocanja
▴ 60
0
votes
1
reply
233
views
How to select cells that represents more accurately a cluster in a scRNA-seq dataset?
scRNAseq
updated 7 weeks ago by
ATpoint
★ 4.0k • written 7 weeks ago by
sodiogoes
• 0
1
vote
2
replies
742
views
ScRNAseq analysis scran :: quickcluster Error
scRNAseq
quickcluster
scRNA
scran
4 months ago
ruba-mahmoud
• 0
0
votes
2
replies
472
views
scRNA analysis : DEG between low and high-expressing cells
scRNAseq
6 months ago
Momo
• 0
0
votes
1
reply
376
views
FALSE error when checking a pre made sample data
Rstudio
scRNAseq
DESeq2
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
DrFizz
• 0
1
vote
5
replies
823
views
Recommendation for large.n in filterByExpr
scRNAseq
edgeR
SingleCellExperiment
8 months ago
duez.tolga
• 0
0
votes
1
reply
967
views
DESeq2 error after installing BiocManager
scRNAseq
RStudio
DESeq2
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
DrFizz
• 0
4
votes
3
replies
1.0k
views
Custom design for scRNA DGE with pseudobulking
scRNAseq
scrna
paireddesign
edgeR
pseudobulking
updated 8 months ago by
Gordon Smyth
50k • written 8 months ago by
garcia
• 0
0
votes
1
reply
402
views
If highly-expressed genes are upregulated, what happens to genes with lower expression after normalization?
scRNA
scRNAseq
updated 8 months ago by
ATpoint
★ 4.0k • written 8 months ago by
Omer
• 0
0
votes
1
reply
544
views
library(topGO) query
topGO
scRNAseq
updated 9 months ago by
Basti
▴ 770 • written 9 months ago by
Francesco
• 0
0
votes
0
replies
340
views
adding noise to scRNA Seq counts
scRNAseq
9 months ago
Jiyuan
• 0
1
vote
11
replies
3.3k
views
Error: Bioconductor version cannot be validated; no internet connection?
scRNAseq
updated 5 months ago by
Manuel
• 0 • written 9 months ago by
Elham
• 0
2
votes
3
replies
1.3k
views
P-value histogram of testLinearModel is not uniform
smart-seq2
scRNAseq
scran
10 months ago
Lluís Revilla Sancho
▴ 730
0
votes
0
replies
458
views
News:
course - single-cell RNAseq data analysis with R and Bioconductor
Workshop
scRNAseq
11 months ago
info
▴ 10
0
votes
1
reply
560
views
scRNAseq Download Errors
scRNAseq
updated 12 months ago by
shepherl
3.8k • written 12 months ago by
Dario Strbenac
★ 1.5k
2
votes
0
replies
1.1k
views
How to aggregate pseudobulks: Normalization & Log-Transformation
pseudobulk
scRNAseq
GSVA
12 months ago
Tadeoye
▴ 20
0
votes
1
reply
710
views
scRNAseq package and cell type annotations
ExperimentHub
R
ExperimentHubData
scRNAseq
SingleR
updated 13 months ago by
Peter Hickey
▴ 740 • written 13 months ago by
cathalgking
▴ 10
4
votes
5
replies
1.3k
views
Pseudo-bulk celltype comparison across conditions from multiple-sources
DESeq2
edgeR
scRNAseq
updated 13 months ago by
Peter Hickey
▴ 740 • written 13 months ago by
EspressoKris
▴ 10
0
votes
2
replies
661
views
Could not find function "Standard Chromosomes"
scrna
scRNAseq
updated 5 months ago by
Gabriella
• 0 • written 13 months ago by
sarah.jobbins
• 0
0
votes
2
replies
871
views
Single-Cell Data Simulation from Bulk RNA-seq
SingleCell
RNASeq
RNASeqData
scRNAseq
updated 2 days ago by
wichaidi
• 0 • written 17 months ago by
irvin.ng
• 0
1
vote
2
replies
1.1k
views
What number components should I use if I want to show 2D and 3D UMAP
scater
UAMP
scRNAseq
18 months ago
Guandong Shang
▴ 40
1
vote
2
replies
934
views
Combine scRNAseq data for bulk analysis using DESeq2
DESeq2
scRNAseq
Fluidigm
18 months ago
evocanres
• 0
0
votes
3
replies
1.0k
views
scRNA-seq: RS4' object has no attribute 'T'" in fastMNN R function
scanpy
scRNAseq
fastmnn
r
18 months ago
Madiha
• 0
4
votes
5
replies
2.2k
views
`emptyDrops()` calling 'too many' non-empty droplets
DropletUtils
emptyDrops
scRNA
scRNAseq
10x
20 months ago
Peter Hickey
▴ 740
4
votes
2
replies
1.1k
views
how to do downstram analysis to cells which are subsetted after MNN batch-correction
batchelor
fastMNN
scRNAseq
20 months ago
Guandong Shang
▴ 40
0
votes
4
replies
2.0k
views
how to make the "reconstructed" value in intergated scRNA-seq UMAP plot more readable
scater
batchelor
scRNAseq
fastMNN
updated 20 months ago by
Aaron Lun
★ 28k • written 20 months ago by
Guandong Shang
▴ 40
0
votes
0
replies
959
views
Job:
Scientific Programmer | Altos Labs | San Diego
SpatialData
scRNAseq
JobPosting
20 months ago
peter.kharchenko
▴ 30
0
votes
0
replies
702
views
Job:
Postdoc position in integrated omics at Karolinska Institute, Stockholm
ATACSeq
multiOmicsViz
scRNAseq
JobPosting
21 months ago
Sebastian Lewandowski
• 0
1
vote
4
replies
1.2k
views
Quality control of scRNA-seq data using mitochondrial ratio (drosophila)
Drosophila_melanogaster
scRNAseq
21 months ago
Chise
▴ 10
0
votes
0
replies
942
views
how to calculate similarity between each cluster in scRNA and bulk RNA-seq
SingleR
scRNAseq
21 months ago
Guandong Shang
▴ 40
0
votes
2
replies
1.8k
views
calculateQCMetrics defunct, how to calculate the quality metrics by perCellQCMetrics
scRNAseq
updated 21 months ago by
ATpoint
★ 4.0k • written 21 months ago by
otieno43
• 0
0
votes
1
reply
1.0k
views
Error: DESeq(dds , useT = TRUE, minmu = 1e-06, test = "LRT", : likelihood ratio test requires a 'reduced' design, see ?DESeq
scRNAseq
DESeq2
updated 22 months ago by
ATpoint
★ 4.0k • written 22 months ago by
abdi.zhr26
• 0
2
votes
8
replies
1.5k
views
RSEM - tximport; where is effective_length information used? Implications for scRNA?
RSEM
tximport
scRNAseq
DESeq2
22 months ago • updated 21 months ago
Kelen
▴ 10
0
votes
1
reply
700
views
scRNASeq reference at a transcript level
EWCE
scRNAseq
updated 23 months ago by
Basti
▴ 770 • written 23 months ago by
marc.tormo
• 0
1
vote
2
replies
1.7k
views
unbalanced cell count and unbalanced read count across samples in pseudobulk differential expression analysis using scRNA-seq data
scRNAseq
DifferentialExpression
DESeq2
2.0 years ago • updated 24 months ago
Ismail
• 0
1
vote
1
reply
1.7k
views
Can we use 'SCT normalization' to find anchors and 'LogNormalize' for the integration?
Normalization
scRNAseq
updated 2.1 years ago by
Peter Hickey
▴ 740 • written 2.1 years ago by
nitinnarwade1504
• 0
2
votes
2
replies
1.2k
views
scuttle:: librarySizeFactors returning non-finite values
normalizeCounts
scran
librarySizeFactors
scuttle
scRNAseq
2.2 years ago
behr201
• 0
2
votes
2
replies
1.1k
views
How to deal with Expression set assembly problem?
deconvolution
r
scRNAseq
bioinformatics
2.2 years ago
Hasan Alanya
• 0
0
votes
1
reply
949
views
Aberrant number of cells
CellBarcode
DropletUtils
emptyDrops()
Cellranger
scRNAseq
updated 23 months ago by
rohitsatyam102
▴ 20 • written 2.2 years ago by
gregoire.destreel
• 0
0
votes
0
replies
869
views
Job:
Postdoc Opening in Bioinformatics or Computational Biology
SpatialData
scRNAseq
SingleCell
Omixer
2.3 years ago
Sebastian Lewandowski
• 0
0
votes
3
replies
1.5k
views
NA values for mitochondrial gene percentage
Seurat
scRNAseq
2.4 years ago
zahra.mesri95
• 0
0
votes
0
replies
625
views
scRNA technical replicates in separate runs
SingleCellExperiment
scRNAseq
2.4 years ago
Núria
• 0
0
votes
2
replies
908
views
scRNAseq analysis on multiple datasets
scRNAseq
updated 2.6 years ago by
swbarnes2
★ 1.3k • written 2.6 years ago by
SG
• 0
2
votes
4
replies
1.2k
views
edgeR output doubts
logFC
edgeR
DifferentialExpression
scRNAseq
2.7 years ago
mbenegas
• 0
0
votes
1
reply
1.0k
views
Identify a scRNASeq cell-population using a bulk RNA Seq dataset of that population?
RNASeq
rnaseq
scRNAseq
updated 2.7 years ago by
Aaron Lun
★ 28k • written 2.7 years ago by
curiousmind007
▴ 10
0
votes
0
replies
762
views
Correlation plot using the count data from seurat object
scRNAseq
2.7 years ago
Sam
• 0
2
votes
4
replies
3.8k
views
differential expression for Single-cell RNAseq data using edgeR
edgeR
single
scRNAseq
2.7 years ago • updated 2.6 years ago
mbenegas
• 0
2
votes
13
replies
2.2k
views
Running the DESeq2 "DESeqDataSetFromMatrix" function returns a dimname error
DESeq2
Scrna
scRNAseq
2.8 years ago • updated 2.7 years ago
RDoc
▴ 10
0
votes
1
reply
836
views
Tool for simulating bulk RNA-Seq from single cell data
scRNAseq
updated 2.8 years ago by
James W. MacDonald
65k • written 2.8 years ago by
mpatino2799
• 0
50 results • Page
1 of 1
Recent ...
Replies
Comment: How to retrieve detailed regression results from deseq2
by
Weiqian
▴ 10
Hi Michael, thanks for the answer! I'll have a look : )
Comment: DESeq filtering specific to contrasts
by
Michael Love
41k
It will likely not filter it out, but really the per-dataset details would affect the results.
Comment: DESeq filtering specific to contrasts
by
Carlin95
• 0
Thanks for clarifying. Could I ask maybe one more thing for my sanity? Let's say you had a multi-factor experiment with contrasts including…
Comment: FilterByExpr low counts with small sample size
by
Jonathan
▴ 10
Thank you for your answer; However, I have some follow-up questions. Following up in In your [1-2-3 article][1], you say that > "... the…
Comment: DESeq2 a lot of genes showing up as differentially expressed that only have 1 sa
by
Seyram M.
• 0
Thank you very much for your reply! I will do so. Kind regards, Seyram
Votes
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
A: Docker container with bioconductor and devtools
Answer: OrgDb for maize
Comment: significant difference of p-values with dream() after updating VariancePartition
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
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