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seurat
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'Seurat' package
singlecell
seurat
updated 11 months ago by
ATpoint
★ 4.0k • written 11 months ago by
Sooni
▴ 10
2
votes
4
replies
3.7k
views
How to convert large gene counts data into matrix?
R
Bioconductor
singlecell
bioinformatics
seurat
updated 9 weeks ago by
Gordon Smyth
50k • written 4.7 years ago by
sambunga094
• 0
1
vote
2
replies
699
views
trying to add SingleR label to Seurat Object fails
SingleR
Seurat
updated 5 months ago by
ATpoint
★ 4.0k • written 5 months ago by
Assa Yeroslaviz
★ 1.5k
0
votes
4
replies
3.6k
views
error in (function (classes, fdef, mtable) unable to find an inherited method for function logNormCounts for signature Seurat
seurat
singleR
updated 10 months ago by
Marco
• 0 • written 4.4 years ago by
mathai.c
• 0
0
votes
5
replies
747
views
scRNA-seq as pseudobulk for DEseq2: does AggregateExpression() normalize counts?
pseudobulk
seurat
DESeq2
updated 4 months ago by
Kevin Blighe
★ 3.9k • written 4 months ago by
daiane.hemerichbrennan
• 0
5 results • Page
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Answer: rlog transformation
by
Michael Love
41k
It is dependent on the other samples in the dataset.
Answer: ONT long-read RNA sequencing - data analysis options
by
Michael Love
41k
*"This worked really well for brain but has not work as well for lung as I get #N/A for many padj"* See the vignette for the cause of th…
Answer: DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
by
Michael Love
41k
Starting from the very top, do you understand the error? You need to provide sample info that matches 1-to-1 the columns of the counts matr…
Answer: covariate with negative and positive values
by
Michael Love
41k
Not a problem. However, if the values are very large (eg 1e6) or very small (eg 1e-6), it's a good idea to center and scale the variable…
Comment: FilterByExpr low counts with small sample size
by
Jonathan
▴ 10
Usually I run `voomLmFit`, although in order to create the mean-variance trend plots for this particular post, I ran `voom`; I've checked -…
Votes
Comment: Error in champ.load(): The following specified files do not exist
Answer: Error in read.metharray(basenames = files, extended = extended, verbose = verbos
Using GRanges and IRanges to simply get all chromosome data
A: Using GRanges and IRanges to simply get all chromosome data
Comment: Reading huge bismark coverage files using bbseq::read.bismark
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