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written 14.0 years ago by
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Answer: Correct the batch effect in methylation analysis in R
by
Gordon Smyth
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You should not apply batch correction functions like harman before a limma analysis. Instead, just add `batch` to the limma linear model.
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Answer: Agilent Microarray Gene Oncology analysis gone wrong
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Gordon Smyth
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Your use of `coef=1` is incorrect, for reasons explained in the limma User's Guide. If you had simply left `coef` at the default, then thin…
Comment: Normalised counts from RUVg in RUVSeq
by
Shaimaa Gamal
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0
Hello
I was wondering if I should use ruv-corrected counts and ruv lib sizes to create a DGE object that can be used later for DE analysis …
Comment: enrichGOgradient color change
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James W. MacDonald
64k
You could look at my answer and extrapolate from there.
Comment: enrichGOgradient color change
by
WU
•
0
I would like to ask how to make the counts legend black. Thank you.
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